DNA chips or biochips, also known as gene chips, which are DNA hybridization probe and the semiconductor industry is combined with crystallization. The technology refers to a large number of probe molecules fixed on supports with a fluorescent marker after the DNA samples were hybridized molecules, molecules by detecting hybridization of each probe to obtain samples of molecular signal strength and thus the number and sequence information.
VenaECTM Biochip: For cell-cell interaction studies under conditions mimicking physiological flow TM VenaEC Biochips contain tissue culture treated substrates which enable the seeding and culturing of endothelial cells with 2 parallel microcapillaries which are sealed on top via a customised frame. Cells derived from different endothelial tissues can be grown easily on the VenaEC Biochips. Cell suspensions may then be injected using the MirusTM Nanopump which supports a range of shear stresses for dynamic flow based assays. VenaECTM Biochips are supplied with 4 substrates and in packs of 10, facilitating 80 experiments per pack. Features • Suitable for a wide range of cell suspensions. • Easy to seed substrates with a range of different endothelial cells. • Biochip plastic is optically clear permitting detailed microscopy studies. • 0.05 – 20 dyne/cm2 shear stresses easily obtained and controlled by the MirusTM Nanopump and FlowAssay software. • Shear stress may be preset to be incrementally increased during an assay. • Real time imaging under flow conditions. • Suitable for use with whole blood. Performance Specifications Endothelial Cell Seeding of Substrate: Range of endothelial cells Range of endothelial cells lines; Human Coronary Artery Edothelial Cells (HCAEC); Human Microvascular Endothelial Cells (HMVEC). Cell Suspension Assay Cell types • T-cells: primary & cell lines e.g. Hut-78 • Monocytes: primary and cell lines; e.g. THP1 • Eosinophil: primary • Neutrophil: primary • PBMCs, whole blood Minimum Sample Volume 12µl Maximum Sample Volume 100µl Shear Stress Range* 0.05 – 20 dyne/cm2; steps of 0.05 dyne/cm2 (100 µL syringe) Shear Stress Range for whole blood* 2.25 - 200 dyne/cm2 (1 mL syringe) Volumetric Flow Rates 100nL/minute - 20 µL/minute (100 µL syringe) Sample Volume Aspiration Accuracy ±1% Shear Stress Accuracy ±0.5% Sample Volume Aspiration Precision <1% CV Shear Stress Precision <0.5% CV 2 *1mL syringe for high shear stress > 10 dyne/cm . **Given for the flow of distilled water in a microcapillary with dimensions: 600 µm (W) x 120 µm (D) x 20mm (L). Technical Specifications Number of channels per biochip 2 Number of substrates supplied with biochip 4 Volume of each channel 1.44 µl Dimensions of each channel 600 µm (W) x 120 µm (D) x 20mm (L) Dead volume at input port 0.1 µl Thickness of bottom substrate 1.0mm Cellix Ltd., Unit 3.81 Institute of Molecular Medicine, Trinity Centre for Health Sciences, James Street, Dublin 8, Ireland Ph: 00 353 1 896 27 99 Fax: 00 353 1 896 27 71 URL: www.cellixltd.com e-mail: firstname.lastname@example.org
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