VenaEC Biochip_ by jlhd32

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									                                               VenaECTM Biochip:
For cell-cell interaction studies under conditions mimicking
                       physiological flow
          TM
VenaEC Biochips contain tissue culture treated substrates which
enable the seeding and culturing of endothelial cells with 2 parallel
microcapillaries which are sealed on top via a customised frame. Cells
derived from different endothelial tissues can be grown easily on the
VenaEC Biochips. Cell suspensions may then be injected using the
MirusTM Nanopump which supports a range of shear stresses for
dynamic flow based assays.

VenaECTM Biochips are supplied with 4 substrates and in packs of 10,
facilitating 80 experiments per pack.



    Features
•    Suitable for a wide range of cell suspensions.
•    Easy to seed substrates with a range of different endothelial cells.
•    Biochip plastic is optically clear permitting detailed microscopy studies.
•    0.05 – 20 dyne/cm2 shear stresses easily obtained and controlled by the MirusTM Nanopump and FlowAssay
     software.
•    Shear stress may be preset to be incrementally increased during an assay.
•    Real time imaging under flow conditions.
•    Suitable for use with whole blood.

Performance Specifications
Endothelial Cell Seeding of Substrate:
Range of endothelial cells                                   Range of endothelial cells lines; Human Coronary Artery Edothelial
                                                             Cells (HCAEC); Human Microvascular Endothelial Cells (HMVEC).

Cell Suspension Assay
Cell types                                                   • T-cells: primary & cell lines e.g. Hut-78
                                                             • Monocytes: primary and cell lines; e.g. THP1
                                                             • Eosinophil: primary
                                                             • Neutrophil: primary
                                                             • PBMCs, whole blood
Minimum Sample Volume                                        12µl
Maximum Sample Volume                                        100µl
Shear Stress Range*                                          0.05 – 20 dyne/cm2; steps of 0.05 dyne/cm2 (100 µL syringe)
 Shear Stress Range for whole blood*                         2.25 - 200 dyne/cm2 (1 mL syringe)
Volumetric Flow Rates                                        100nL/minute - 20 µL/minute (100 µL syringe)
Sample Volume Aspiration Accuracy                            ±1%
Shear Stress Accuracy                                        ±0.5%
Sample Volume Aspiration Precision                           <1% CV
Shear Stress Precision                                       <0.5% CV
                                                 2
*1mL syringe for high shear stress > 10 dyne/cm .
**Given for the flow of distilled water in a microcapillary with dimensions: 600 µm (W) x 120 µm (D) x 20mm (L).

Technical Specifications
Number of channels per biochip                               2
Number of substrates supplied with biochip                   4
Volume of each channel                                       1.44 µl
Dimensions of each channel                                   600 µm (W) x 120 µm (D) x 20mm (L)
Dead volume at input port                                    0.1 µl
Thickness of bottom substrate                                1.0mm

Cellix Ltd., Unit 3.81 Institute of Molecular Medicine, Trinity Centre for Health Sciences, James Street, Dublin 8, Ireland
Ph: 00 353 1 896 27 99 Fax: 00 353 1 896 27 71 URL: www.cellixltd.com e-mail: r.ashe@cellixltd.com

								
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