MPL PROPOSAL RISK ASSESSMENT by lzv41816

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									                                                                                                   Doc No: MPL Proposal Risk
                                                                                                         Assessment V2
              MPL Proposal
                                                                                                       Date: 2nd June 2008
             Risk Assessment
                                                                                                           Page: 1 of 4



                                   MPL PROPOSAL RISK ASSESSMENT


PLEASE COMPLETE THIS FORM TO ENABLE AN INITIAL ASSESSMENT OF THE SAFETY ISSUES
ASSOCIATED WITH YOUR EXPERIMENT. YOU MAY BE ASKED TO A COMPLETE FURTHER
ASSESSMENT IF YOU ARE AWARDED TIME AT THE MPL.

Note 1: Please be aware that the Membrane Protein Laboratory and Diamond Light Source are only licensed for
Containment Level 1, this means we cannot currently accept any projects involving human (ACDP List) or animal
(SAPO List) pathogens of higher than Hazard Group 1 including most human tissues, genetically modified organisms
assessed as a class higher than 1 (GM Risk Assessment) or any toxins or micro-organisms covered by Schedule 5 of
the Anti-Terrorism Crime and Security Act 2001 (ATCSA 2001). To assess whether your project involves any of
these categories please refer to the following websites:

             Schedule 5 to ATCSA 2001: http://security.homeoffice.gov.uk/news-
              publications/publication-search/anti-terrorism-crime-
              securityact/ATCSA_2001_Schedule_5_Order.pdf?view=Binary
             ACDP List Biological Agents: http://www.hse.gov.uk/pubns/misc208.pdf
             Specified Animal Pathogens Order 1998 (SAPO 1998):
              http://www.defra.gov.uk/animalh/diseases/pathogens/classification.htm



Note 2: Users are responsible for HSE / DEFRA / Home Office license cover for transport of samples to and from
Diamond Light Source Ltd.




 Principal investigator’s name:


 Name of scientist proposing to visit the MPL:


 Title of proposal:




1) Do any of the samples you will bring to Diamond include any of the following :

Pathogen from Hazard Group >1 (according to current lists of both ACDP and Specified Animal Pathogens Order
1998)*
 Yes                                                         No
Genetically Modified Organisms Category >GM1 (as assessed by recognised GMSC according to ACGM guidance)*
 Yes                                                     No
 Micro-organisms or toxins covered by Schedule 5 (current version) of Anti-Terrorism, Crime and Security Act 2001*
 Yes                                                        No
 Human Tissue samples other than hair samples or nail clippings*
 Yes                                                         No

*) Please note that if you have replied ‘Yes’ to any of the above questions we will not at this moment be able to accommodate
your project at MPL / Diamond since we currently do not have the facilities to accommodate projects higher than Containment
Level 1. Although in the future these arrangements may change, this would require significant modifications. It is therefore
unlikely that a project of this type would be given time at the MPL in the first application rounds.
                                                                                           Doc No: MPL Proposal Risk
                                                                                                 Assessment V2
           MPL Proposal
                                                                                               Date: 2nd June 2008
          Risk Assessment
                                                                                                   Page: 2 of 4



2) Please provide details of the samples you propose to bring to the MPL:

 Plasmid or expression vector, as purified DNA                                           Yes              No
 If yes, please provide details, including the intended cell lines you will transform with this vector:

 Genetically modified organisms for expression of protein in the MPL                     Yes               No
 If yes, please provide details of the cell line (including phenotype) and vector (including antibiotic resistance markers
 and other genes on the plasmid):

 Wild type organism for purification from the native source                          Yes            No
 If yes, please provide details, including the name of the organism and any associated hazard categories:

 Cell pellets                                                                            Yes             No
 If yes, please provide details, including cell line, phenotype, vector and antibiotic markers. Please also include
 information on whether you have confirmed that there are no live cells present. If live cells could be present you will
 need to complete a GMO assessment:

 Pure protein                                                                          Yes              No
 If yes, please provide full details, including protein name, function, source organism and its hazard group, expression
 system, ligands, substrates, buffers or other reagents in the sample:

 Protein crystals                                                                         Yes             No
 If yes, please provide details:

 Other                                                                              Yes            No
 If yes, please provide details including the name of the organism and any associated hazard categories:



3) Sample storage:

 Do you have any specialist storage needs for your samples?                              Yes              No
 If yes, please provide details:



4) Which other specific hazards are associated with your sample?

 Pathogenic to plants or environment                            Yes                                       No
 Non-human tissue sample                                        Yes                                       No
 Viable organism                                                Yes                                       No
 Virulence factor / toxin                                       Yes                                       No
 Prion or part of prion                                         Yes                                       No
 Hazardous ligand                                               Yes                                       No
 Radioactivity*                                                 Yes                                       No
 Flammable / Explosive                                          Yes                                       No
 Irritant / Harmful                                             Yes                                       No
 Dust                                                           Yes                                       No
 Corrosive                                                      Yes                                       No
 Reactive with air/oxygen/water/acid                            Yes                                       No
 Toxic / very toxic                                             Yes                                       No
 Mutagenic                                                      Yes                                       No
 Carcinogenic                                                   Yes                                       No
 Teratogenic                                                    Yes                                       No
 Sensitising / Allergenic                                       Yes                                       No
 Is designated a Work Exposure Limit (WEL)**                    Yes                                       No
 Potential contamination                                        Yes                                       No
                                                                                                             Doc No: MPL Proposal Risk
                                                                                                                   Assessment V2
             MPL Proposal
                                                                                                                 Date: 2nd June 2008
            Risk Assessment
                                                                                                                     Page: 3 of 4



 Other hazard                                                                Yes                                                No
 If you have answered ‘YES’ to any of the above, please provide details for each relevant sample/substance including
 quantities:


 *Please provide details of isotopes, number of samples, total activity (Bq), sealed/unsealed form (solid, powder or liquid) and specify if samples
 contain uranium, thorium or plutonium.
 ** Any substance/material which has a WEL given in the current HSE Guidance Notes EH40 is a substance hazardous to health - please explain
 whether limit likely to be reached under normal/emergency conditions.




5) Any other hazards associated with experiment, equipment use or sample preparation at Diamond:

 Lasers                                                                      Yes       (specify class below)                    No
 Gases                                                                      Yes                                                 No
 Robots (*)                                                                 Yes                                                 No
 High Voltage / Electricity                                                 Yes                                                 No
 Pressurised vessels                                                        Yes                                                 No
 No BS / EN standards (e.g. Made-In-House)                                  Yes                                                 No
 High Temperature                                                           Yes                                                 No
 New Personnel (please inform us of training needs)                         Yes                                                 No
 Other                                                                      Yes                                                 No
 (*) The MPL has a crystallisation robot, which may be used for crystallisation experiments. This robot has an interlock system which prevents users
 from entering the robot enclosure during operation. The liquid handler robot has a door which prevents users accessing the system during
 experimental runs. User training is in place to ensure that all users are informed of the risks and trained to use the robot in a safe way.
 Please describe any additional hazards:




6) Which controls will you require to be in place:

 Special Transport (to / from Diamond, to be provided by User) Yes                                                             No
 Special Transport (inside Diamond)                            Yes                                                             No
 Secure Storage                                                Yes                                                             No
 Restricted Access                                             Yes                                                             No
 General Ventilation                                           Yes                                                             No
 Local Exhaust Ventilation                                     Yes                                                             No
 Biological Containment L2 / 3                                 Yes                                                             No
 Microbiological Safety Cabinet Class I / II / III             Yes                                                             No
 Personal Protective Equipment (eg gloves, face mask, lab coat Yes                                                             No
 Training                                                      Yes                                                             No
 None of the above
 If you have answered ‘YES’ to any of the above, please provide details:




7) After your visit to the MPL

 Will you require your samples to remain at MPL after your visit? Yes                           No, samples will be removed
 If yes, please give details if you require any of your samples to be left stored in the crystallisation hotels or in the
 fridges or freezers in a clearly labelled box with the name contact details and MPL contact information:
                                                               Doc No: MPL Proposal Risk
                                                                     Assessment V2
           MPL Proposal
                                                                   Date: 2nd June 2008
          Risk Assessment
                                                                       Page: 4 of 4




8) Approval

 Signature of Principal Investigator:

 Print Name:

 Date:

 Contact email and tel. number in case of queries:



 Signature of Principal Beamline Scientist/MPL group leader:

 Print Name:

 Date:

								
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