Direct Digital Counting of Radioisotopes for Thin Layer Chromatography

							 Direct Digital Counting of Radioisotopes for
   Thin Layer Chromatography Applications

 I No cutting or scraping—no sample preparation hazards
I Fast, automated 1D and 2D image acquisition and analysis
 I Accurate, reliable gas proportional detection technology
          Direct Digital Quantitation
Windowless Gas Proportional
Detection Technology                                            No Sample
                                                                Prep Hazards!
• Windowless—allows sensitive tritium detection and dual
label measurements                                              • No cutting, scraping
                                                                or scintillation
• Resolution—selectable collimators allow optimal
                                                                cocktails
resolution and speeds for each application, with positional
                                                                to add
accuracy of less than 1 mm in the Y-axis and mechanical
                                                                • Minimize
precision of 0.1 mm in the X-axis                               technician
• Linearity—4-5 decades of linear detection for up to           exposure and
1000 times the range of X-ray film without exposure             get data in the
guesswork and potential saturation                              time usually
                                                                spent at the fume
• High Sensitivity—detect and quantify less than 100 dpm
                                                                hood scraping plates
of 14C per lane for metabolite analysis in less than 10
                                                                • High sensitivity
minutes                                                         counting for all
• Flexibility—measures beta (3H, 14C, 35S), gamma (99mTc) and   isotopes, including
positron (18F, 11C) emitters with no hardware                   tritium
modifications or additional detectors                           • Automated acquisition of TLC
                                                                lanes or 2D images, from a
                                                                single lane, to a model that
                                                                holds three 20 x 20 cm TLC
                                                                plates
                                                                • No contaminated screens and no liquid waste
                       Self-adjusting        Detector anode     disposal
                       detector arm          wire, surrounded
                                             by counting gas                      Ionization of
                                             (not to scale)
     Electronics                                                                  counting gas by
     circuit board                                                                interaction of
                                                                                  radioactivity with
                                                                                  electrons in gas

                                                                                                       Planar sample
                                                                                                       (TLC plate or
                                                                                                       strips)
                                                                                                       containing
                                                                                                       radioactivity




                                                                                                            Sliding tabs for
                                                                                                            automatic
                                                                                                            lane scanning
     Easy to use
     front access
     instrument
     control
Easy-to-Use Software

     WinScan Software
     Provides easy-to-use interface for AR-2000 operation and
     radio-chromatographic analysis, including instrument control,
     method definition and storage, data acquisition, quantitative
     analysis and reporting functions.
     Features include :
     I Real time data display and on-screen analysis tools, including
       ROI definition, background subtraction, % total chromatogram
       activity and Rf values
     I Automatic raw data storage for GLP
     I Automatic peak finding with user adjustable selection criteria
     I User defined printed or exported reports with associated
        acquisition parameters for quality control
     I Plot to scale (1:1) printing option for plate overlays

     WinScan 2D Imaging Software
     Provides graphical image display and analysis for two-
     dimensional radio-chromatography samples, including:
     I Quantitative ROI analysis using on-screen drawing tools
       with smoothing, background subtraction and 1D
       chromatogram overlays for selected lane areas
     I Programmable stepping and flexible collimator selection for
       optimal sensitivity, resolution and scanning time
     I Printing of images, associated data and acquisition
       parameters, or exporting of Windows metafiles or tab-
       delimited Excel compatible data tables
     I Includes additional 6mm high efficiency collimator

     Bio-Chrom Software for
     21 CFR Part 11 Compliance
     Comprehensive radio-chromatography acquisition and analysis
     software designed for Bioscan instruments, which may be used
     in place of WinScan.
     Bio-Chrom Plus features include:
     I 21 CFR Part 11 compliance features, including access control
        and electronic signature tracking
     I Audit trails for GLP compliance
     I Real time data display and analysis
     I Automatic peak detection and auto-scaling display of
        chromatograms
     I Intuitive method set-up, storage and editing functions
     I Extensive evaluation, data export and reporting options
     I Half-life correction for short-lived isotope quantitation
     Bio-Chrom Lite is the lower cost version available for non-
     regulated laboratories and does not include 21 CFR part 11
     compliance features. Bio-Chrom Lite and Bio-Chrom Plus are
     compatible with all Bioscan HPLC and TLC detection
     instruments for a consistent user interface and reduced
     training requirements.
AR-2000 Imaging Scanner for radio-TLC

Applications:                                                             Specifications:
PET / Nuclear Medicine:                                                   Sensitivity (10 min. analysis):
Fast, accurate results for in vivo SPECT or PET                                    1,000 DPM for 3H, 125I
radiopharmaceutical compounds labeled with 18F, 11C,                               100 DPM for 14C, 32P and most other
99m
   Tc, 111In, etc.                                                                 isotopes
• Radiopharmaceutical quality control
                                                                          Resolution:
• Synthesis process control
                                                                                   0.5—1mm for 3H
Pharmaceutical metabolite analysis:                                                1 – 2mm for 14C
Radioisotope flexibility and manual or automatic peak                              3mm for 32P and most gamma emitters
analysis for low or high activity products, using beta (3H,               Linearity:
14
  C), gamma or positron-labeled compounds.                                         Calibrated to within 1% (± 2mm)
                                                                          Background:
Radiotracer toxicology studies:                                                    Less than 0.15 CPM per mm
High sensitivity for quantitative measurement of                          Dimensions (in / cm):
14
  C-labeled organic compounds and agrichemicals.                                   1-Plate: 16 x 9 x 19 / 40 x 23 x 48
                                                                                   2-Plate: 24 x 9 x 19 / 61 x 23 x 48
Lipid biosynthesis / Lipid analysis:                                               3-Plate: 32 x 9 x 19 / 81 x 23 x 48
1D and 2D analysis of complex lipids, phospholipids and
                                                                          Weight (lbs / kg):
glycolipids by TLC with no cutting, scraping or transfer for
fast quantitative results.                                                         1-Plate: 33 / 15
                                                                                   2-Plate: 39 / 18
Radiochemical purity:                                                              3-Plate: 45 / 20
Rapid quality assurance results for batch validation of                   Power:
radiolabeled compounds for tracer or metabolism                                    110/220 V, 50/60 Hz
studies.                                                                  Operating system:
                                                                                   Pentium computer, Windows 98/2000/XP,
Radiolabeled reporter gene or enzyme assays:                                       one 9-pin serial port, at least 64 Mb RAM
Simplifies the analysis and improves accuracy for                                  (128 Mb preferred)
traditional radioisotopic CAT or enzymatic conversion                     Gas Supply / Flow Rates:
assays using TLC.                                                                  P10 counting gas (90% Argon/10%
                                                                                   Methane) regulated to 10—20 psi
Quantitative biochemical separations and planar
samples using radiolabeled compounds:                                     Environmental:
Programmable scanning and quick-change magnetic                                    Temperature range: 10—40°C
collimators for resolution and sensitivity optimization.                           Humidity: less than 75% (non-condensing)

Three models provide 1, 2 or 3 TLC plate capacity




Bioscan Inc.                                          Your local representative                               Bioscan Europe Ltd.
                                                                                                                                       D•ACom Neuves-Maisons France - RCS Nancy B 443 901 715 - 2004/06




4590 MacArthur Blvd., N.W.                                                                                 58, Avenue de Wagram
Washington, DC 20007 USA                                                                                      75017 Paris - France
Toll-free (U.S. and Canada)                                                                       Phone/Fax : + 33 3 83 63 62 67
1-800-255-7226                                                                                       Email : europe@bioscan.com
Phone : 1-202-338-0974
Fax : 1-202-333-8514                                                                    Please visit our website for information on
Email : sales@bioscan.com                                                                                  your local representative
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