ANALYTICAL TOOLS FOR CONFOCAL MICROSCOPY
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ANALYTICAL TOOLS FOR CONFOCAL MICROSCOPY Martin Hoppe, Lioba Kuschel Leica Microsystems CMS GmbH Am Friedensplatz 3 D 68165 Mannheim, Germany E-mail: firstname.lastname@example.org email@example.com Key words: Spectral Confocal Microscopy, FLIM, FCS, live cell imaging, two photon microscopy Leica Microsystems beam scanning laser confocal microscopes have recently been extended beyond imaging to serve as analytical tools in Molecular and Cell Biology as well. The concept of integrating and optimizing FLIM (Fluorescence Lifetime Imaging) and FCS (Fluorescence Correlation Spectroscopy) in the Leica TCS SP2 AOBS Spectral Confocal Microscope is discussed. The precision and flexibility of integration allows coping with a wide range of experimental conditions, which is demonstrated by a variety of application examples. Additionally, recent enhancements of sensitivity of the confocal optics and detection system for low-level live cell and molecular imaging and for maximizing penetration depth in two photon microscopy are discussed.