1
13
2
12
3 4 5
11 10 6 7
9 base scanning objective lens revolving nosepiece low power objective lens
8 diaphragm body tube stage stage clips coarse adjustment knob high power objective lens Light source ocular (eyepiece) fine adjustment knob
Function/Purpose: 1)_____________________________________________________________________________ 2)_____________________________________________________________________________ 3)_____________________________________________________________________________ 4)_____________________________________________________________________________ 5)_____________________________________________________________________________ 6)_____________________________________________________________________________ 7)_____________________________________________________________________________ 8)_____________________________________________________________________________ 9)_____________________________________________________________________________ 10)____________________________________________________________________________ 11)____________________________________________________________________________ 12)____________________________________________________________________________ 13)____________________________________________________________________________
Rules for Storing Microscopes:
The microscopes used in this course are expensive and should be cared for properly. Please obey the following rules when using microscopes:
1. Always carry your scope with ______ hands: One hand on the __________ and the other under the _______________. 2. Place your scope on the table ____________ from the edge. 3. When finished with the scope make sure you do the following before storing it: a. Wipe the objectives with ____________________________ (Other cloth/tissue may scratch the lens) b. Rotate the nosepiece so that the ____________________________lens is pointing down. c. Carefully ___________ the cord around the arm and above the _____________ ___________________. d. Place the ________________ over the scope. h. Store the scope in the proper compartment in the cabinet.
Activity Questions: Choose a slide from the table, following the proper viewing techniques presented by Ms. Z, observe the slide under the low power objective: 1) What is the # on the slide you are viewing? ______________________________ 2) What do you think this specimen is? ____________________________________ ____________________________________________________________________ 3) Diagram what you see in the field of view: 4) Calculate the total magnification: Ocular magnification (A) = _____________
Low power objective magnification (B) = _____________ Total Magnification (A x B) = ________________________
5) Now observe your slide under the high power objective (remember the rules!) Diagram what you see in the field of view: 6) Calculate the total magnification: Ocular magnification (A) = ____________
High power objective magnification (B) = ____________ Total Magnification (A x B) = ____________________
7) Adjust the diaphragm, slowly, both ways while looking at your specimen. a) How does the image of your specimen change? _______________________________________ _______________________________________________________________________________ b) What does the diaphragm do? (What is its function?)___________________________________ _______________________________________________________________________________ 8) Put your hand over the lamp, (BUT DON’T TOUCH IT!!) a) Can you feel a temperature difference? _________ What is the difference (Warmer or Cooler?) b) Can you hypothesize why it wouldn’t be a good idea to leave a live specimen on the microscope for too long with the light on? What might happen to it? _________________________ _______________________________________________________________________________