Posters 1. AfCS Antibody Laboratory's Database. Tabulation of Our Experience with Commercially Available Antibodies Heping Han, Becky Fulin, Lonnie Sorrells, Ruth Levitz, and Susanne Mumby 2. Cultured Bcl-2 Transgenic B-Cells as a Signaling Model for Primary B Cells Tamara Roach, Sangdun Choi, and Heping Han 3. Identification of Immuno-Affinity Isolated Phosphotyrosine Proteins from WEHI 231 cells Shu H, Chen S, Lyons K, Hsueh R, and Brekken D 4. Identification of the In Vivo Phosphorylation Sites in Murine Leukocyte-Specific Protein 1 Shu H, Chen S, DeCamp D, Hsueh R, and Brekken D 5. Enrichment and Identification of WEHI 231 Phosphoproteins using IMAC and LC-MS/MS Shu H, Chen S, Hsueh R, and Brekken D 6. Multiplexed Lipid Arrays of Ligand-Induced Changes in Glycerophospholipid Composition of WEHI-231 Cells (Part 1). Jeffrey S. Forrester, Stephen B. Milne, Pavlina T. Ivanova, Michelle D. Armstrong, and H. Alex Brown 7. Multiplexed Lipid Arrays of Ligand-Induced Changes in Glycerophospholipid Composition of WEHI-231 Cells (Part 2). Stephen B. Milne, Jeffrey S. Forrester, Pavlina T. Ivanova, Michelle D. Armstrong, and H. Alex Brown 8. Multiplexed Lipid Arrays of Ligand-Induced Changes in Glycerophospholipid Composition of WEHI-231 Cells (Part 2 continued). Stephen B. Milne, Jeffrey S. Forrester, Pavlina T. Ivanova, Michelle D Armstrong, and H. Alex Brown 9. Preparation and Characterization of WEHI-231 for the Ligand Screens Robert Hsueh, Keng-Mean Lin, and Heping Han 10. Isolation and Culture of Adult Mouse Cardiac Myocytes for Signaling Studies Timothy D. O'Connell, Yan G. Ni, Keng-Mean Lin, Heping Han, and Zhen Yan 11. Ligand Screen in Mouse Adult Cardiac Myocytes Yan G. Ni, Keng-Mean Lin, Heping Han, and Timothy D. O'Connell
12. Building an Atlas of Subcellular Localization Markers in WEHI 231 Cells T Mukai, Q Mi, J Zavzavadjian, E Gehrig, M Verghese, E Fung, S Couture, WS Park, N O'Rourke, I Fraser, and G Chandy 13. Development of a Protein Kinase Localization Map M. Verghese, J. Zavzavadjian, T. Mukai, E. Gehrig, S. Couture, W. Park, A. Maer, S. Lim, B. Saunders, N. O'Rourke, I. Fraser, and G. Chandy 14. Localization and Translocation of PH Domains in the WEHI 231 B Cell Line Takako Mukai, Wei Sun Park, Mary Verghese, Liz Gehrig, Grischa Chandy, and Nancy O'Rourke 15. ESCAT Bridging Project Tobias Meyer 16. Development of the Protein Trap Assay for WEHI-231 Dianne DeCamp, Iain Fraser, Hongjun Shu, and Yan G. Ni 17. Phosphorylation Indicators Jin Zhang, Christopher Hupfeld, Jerrold Olefsky, and Roger Tsien 18. Monomeric Coral-Derived Fluorescent Proteins Robert Campbell, Nathan Shaner, and Roger Tsien 19. Microfabrication-Based Dynamic Array Cytometer (µDAC) Rebecca Braff Maxwell, Antimony L. Gerhardt, Mehmet Toner, Martha L. Gray, and Martin A. Schmidt 20. Visualization of Biochemical Networks in Living Cells Ingrid Remy and Stephen Michnick 21. Dual Ligand Screen in Splenic B Cells Keng-Mean Lin, Madhusudan Natarajan, Robert Hsueh, and Heping Han 22. Dual Ligand Screen in Splenic B Cells (continued) Keng-Mean Lin, Madhusudan Natarajan, Robert Hsueh, and Heping Han 23. RNA Interference Studies in B Lymphocyte and Monocyte Cell Lines Iain Fraser, Joelle Zavzavadjian, Jong-Ik Hwang, Pamela Eversole-Cire, Jamie Liu, Dan Allen, Mei Wang, and Melvin Simon 24. Yeast Two-Hybrid Interactions in B Lymphocytes and Cardiac Myocytes Iain Fraser, Terrece Pearman, Brandi Williams, Bob Sinkovits, Max Dufford, Keng-Mean Lin, Robert Hsueh, Zhen Yan, Joella Grossoehme, Read Pierce, Jason Polasek, Jody Girouard, Karen Heichman, Paul Bartel, and Gil Sambrano
25. The AfCS Plasmid Database: Theory and Practice Joelle R. Zavzavadjian, Stephen Lyon, Jamie Liu, Kavitha Dhandapani, Andreia Maer, Yuhong Ning, Grischa Chandy, and Iain D.C. Fraser 26. A Navigation and Visualization System for Signaling Pathways Gilberto R. Sambrano 27. AfCS Research Reports and Brief Communications Gilberto R. Sambrano 28. Analysis of the Primary B Cell Microarray Data Madhusudan Natarajan and Rama Ranganathan 29. Analysis of B Cell Single Ligand Data Robert Sinkovits and Dennis Mock 30. Generation of Cell Specific Gene Parts List from Array Data Eugene Ke, Chris Benner, Xiaocui Zhu, Becky Hart, Dennis Mock, Mel Simon, and Shankar Subramaniam 31. CLASSIFI: A Microarray Gene Cluster Classification Algorithm Based on Statistical Analysis of Gene-Ontology Annotation Jamie Lee, Robert Sinkovits, Dennis Mock, Eva Rab, Peng Yang, Brian Saunders, Robert Hsueh, Shankar Subramaniam, and Richard H. Scheuermann 32. Cross Comparison of DNA Microarray Platforms Yun Anna Cao, Sun Young Lee, Jong-Woo Kim, Mi Sook Chang, and Sangdun Choi 33. Gene Expression Profiles of Mouse B Cells Stimulated with 32 Ligands Xiaocui Zhu, Rebecca Hart, Jong-Woo Kim, Sun Young Lee, Mi Sook Chang, Yun Anna Cao, Angela Alexander, Joella Grossoehme, Zhen Yan, Robert Hsueh, Paul Sternweis, Sangdun Choi, and Melvin I. Simon 34. Ligand Screen Microarray Data Explorations Gentian Buzi, Christopher Hart, Eric Mjolsness, and Barbara Wold 35. A Framework for Quantitative Comparison and Interactive Exploration of Large Scale Gene Expression Analyses Christopher E. Hart, Eric D. Mjolsness, and Barbara J. Wold
36. Molecule Pages - a Comprehensive Signaling Database Joshua Q. Li, Yuhong Ning, Warren Hedley, Brian Saunders, Timo Hannay, Yongsheng Chen, T.B.K. Reddy, Andreia Maer, Nicole Tindill, Peter Morrison, and Shankar Subramaniam 37. Molecule Pages Database Architecture Joshua Q. Li, Yuhong Ning, Warren Hedley, Yongsheng Chen, Timo Hannay, Nicole Tindill, T.B.K. Reddy, Andreia Maer, Jingwei Meng, and Shankar Subramaniam 38. Laboratory Information Management System for the AfCS Madhusudan, Ilango Vadivelu, Lothar Krause, Stephen Lyon, Peter Morrison, and Shankar Subramaniam 39. Searching the AfCS Database Using Datascope Roger Unwin, Dawn Cotter, Brian Saunders, and Shankar Subramaniam 40. Models of Experiments on Immune Cell Chemotaxis Keith Erichson, Matt Onsum, and Shilpa Shraff 41. Software for Pathway and Functional Genomic Data Alex Gilman, Pat Flaharty, and Adam Arkin 42. Overview of the Systems Biology Markup Language (SBML) and the Systems Biology Workbench (SBW) Michael Hucka, Andrew Finney, Herbert Sauro, Hamid Bolouri, John Doyle, and Hiroaki Kitano 43. SOSTOOLS for Analysis of Biological Models and Experiments Stephen Prajna, Antonis Papachristodoulou, Tau-Mu Yi, and John Doyle