Development of Custom Flow Cytometry Instrumentation James Freyer1
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Development of Custom Flow Cytometry Instrumentation James Freyer1, Steven Graves2 1 Biosciences Division, Los Alamos National Laboratory, MS M888, Los Alamos, NM, 87545, 2Center for Biomedical Engineering, University of New Mexico, Centennial Engineering Center, MSC01 1141, 1 University of New Mexico, Albuquerque, NM, 87131 Commercial flow cytometers and cell sorters have been available for the better part of three decades. Increased applications in the past five years have driven a marked increase in new instruments/companies. Focus areas in flow cytometry instrumentation improvement include low cost and/or smaller instruments, microfluidic designs, higher throughput analysis and sorting, increased analysis parameters, non-optical analysis methods, field-based particle manipulation methods and extension of flow cytometry to both smaller (e.g. molecules) and larger (e.g. cell aggregates) particles. Many of the innovations eventually incorporated in commercial instruments have their genesis in basic R&D pursued by several groups focused on pushing the boundaries of flow cytometry technologies. This workshop will highlight several of the recent technical developments being pursued by non-commercial research groups. Areas to be highlighted include: low cost portable flow cytometry; field-based manipulation (acoustic, dielectrophoretic, inertial); microfluidic systems; full spectral resolution; fluorescence lifetime analysis; Raman scattering analysis; compact digital data systems; and in-line sample preparation methods. The first part of this workshop will consist of several 15-minute presentations describing cutting edge R&D in several of these instrumentation development areas. The second part will be a hands-on demonstration of several new instrument designs, with a group of flow cytometry instrumentation development experts available for demonstrations and to answer questions. This workshop should be of interest to researchers interested in flow cytometry instrumentation development, as well as biomedical researchers and flow core facility operators who want a more in-depth view of the future of flow cytometers.