SYSMEX CDA FOR COUNTING AND SIZING OF CELLS AND

SYSMEX CDA-500 FOR COUNTING AND SIZING OF CELLS AND PARTICLES THEORY Designed for ease of use The CDA-500 has been specifically designed for ease of use. By turning the power on, the CDA-500 automatically performs a self-check and cleaning cycle.To make a measurement simply put your sample in the measurement unit, recall a pre-defined measurement profile then press the COUNT key. The measurement is then completed in a matter of seconds. The CDA-500 can recall a range of pre-set measurement profiles. Different profiles can be set depending on the user's application, saving set-up time when making repeat sample measurements. System diagnostics – during operation the CDA-500 is constantly monitored for measurement errors or faulty operation. For example, if the aperture blocks, an error message informs the operator.. Measurement Principle The CDA-500 uses the electro-zone sensing method for sizing and counting particles.The detector assembly consists of an aperture (opening) through which suspended particles pass. Internal and external electrodes are on either side of the aperture.The detector assembly is immersed in an electrolyte that allows a constant current to flow between the electrodes. Non-conductive particles are suspended in an electrolyte.When a particle passes through the aperture during the measurement process, the particle decreases the current flow, increasing the resistance between the electrodes.The change in resistance causes a change in voltage between the electrodes proportional to the volume of the particle. The quantity of suspension drawn through the aperture is precisely controlled to allow the system to count and size the particles for an exact, reproducible volume. Several thousand particles can be counted and sized per second.This technique offers high sensitivity and excellent resolution because all particles are counted and sized.This method is independent of particle shape, colour, refractive index and density. Data Presentation The user interface provides an easy and convenient way of operating the instrument.The data analysis software enhances the flexibility of the instrument. The CDA-500 offers a variety of reports, from simple short form reports detailing the analysis conditions and key measured parameters, to full particle size distributions displayed in either graphic or tabular format. Analyzing results is simple with the range of data processing features available in the CDA-500 software. Measurements can be over-plotted, as either differential or cumulative data.A zoom facility enables the user to focus on a particular size range.The CDA-500 software allows the averaging of results and the automatic calculation of a variety of statistics. In addition a full range of variance graphs are available enabling the operator to quickly recognize any changes in particle size. The data can be easily exported for use in independent software packages. A key feature is the ability to integrate particle size data into reports by "cutting and pasting". Flexible The CDA-500 has a range of measurement modes that can be tailored to the analysis requirements. s Manometer Mode for measuring the number of particles per unit volume. Time Mode for taking measurements for a specified time. Recount Mode for automatically repeating measurements. s s s A Peak Count Mode for counting the number of particles to a pre-set limit. Particle size distribution by volume. Overlaid particle size distribution curves. APPLICATIONS Applications The CDA-500 can be used in a variety of counting and sizing applications, such as tissue culture cells, blood cells, algae, yeast, plankton, suspensions and spermatozoa. Different aperture diameters are available, to enable the user to select the most suitable one for the size of particles to be analysed. In addition, each of the detectors has multiple selectable analysis ranges to increase resolution. s 50µm aperture for measuring particle diameters between 1.25µm - 20µm s 100µm aperture for measuring s 150µm aperture for measuring particle diameters between 3.00µm - 40µm particle diameters between 4.50µm - 60µm Graphical display of differential data. Particle size distribution by particle diameter. Specifications Measurement principle: Electro-zone sensing for particle counting & sizing Measurable particle size range: ~1µm – 60µm Time for analysis: 10 – 60 seconds per sample Aperture diameters: 50µm, 100µm and 150µm Repeatability – count precision: < 1.0% Repeatability – mean particle diameter: < 0.3% Manometer: Mercury free Measured parameters: Volume (fL) mode Diameter (µm) mode Concentration Concentration Mean volume Mean diameter Peak volume Standard deviation Median volume 90% diameter Maximum volume 50% diameter Middle volume 10% diameter Minimum volume Modal diameter Distribution width Half width Electrolyte – "Cell pack" available in 20litre Cubitainer Cleaner – "Manoresh" available in 5litre Cubitainer Disposable Beakers available in packs of 500 and 3000 Weight Dimensions W x H x Depth (mm) Main unit 252 x 325 x 397 12.5Kg AD Converter 200 x 80 x 280 5.0Kg Power Requirements: 117VAC±10% / 220VAC±10% / 240VAC±10% 50/60Hz Requires stand alone PC, with Windows 95 or 98 operating systems, for data analysis software. Reagents and Accessories Malvern Instruments Limited Enigma Business Park Grovewood Road Malvern Worcs WR14 1XZ U.K. Tel: +44 (0)1684 892456 Fax: +44 (0)1684 892789 Max Power Cons. 105VA 30VA Malvern Instruments S.A. Parc Club de L’Université 30, Rue Jean Rostand 91893 Orsay Cedex France Tél: +33 (1) 69 35 18 00 Fax: +33 (1) 60 19 13 26 Malvern Instruments Inc. 10 Southville Road Southborough MA 01772 U.S.A. Tel: +1 (508) 480-0200 Fax: +1 (508) 460-9692 Malvern Instruments GmbH Rigipsstraße 19 71083 Herrenberg Germany Tel: +49 (0) 7032 97770 Fax: +49 (0) 7032 77854 Malvern Instruments (Asia Pacific) Penthouse 21-11 The Boulevard Mid Valley City Lingkaren Syed Putra 59200 Kuala Lumpur Malaysia Tel/Fax: 603 2283 6139 Malvern Instruments Nordic AB Box 15045, Vallongatan 1 750 15 UPPSALA Sweden Tel: +46 (0) 18 55 24 55 Fax: +46 (0) 18 55 11 14 www.malvern.co.uk Ref: MRK381 Malvern Instruments, a leading developer and manufacturer of particle size analysers, has entered into a strategic alliance with Sysmex Corporation, who are involved with the development, manufacture and sales of integrated in vitro diagnostic (IVD) systems, reagents and software. Sysmex has built an excellent reputation in Japan and around the world for its capabilities in the fields of haematology, coagulation, immuno-chemistry and urinalysis. Sysmex is committed to growth in the IVD market as well as in new market areas such as particle sizing in the industrial market. In order to achieve these goals Sysmex has a number of Global Alliances with industry leading companies, to obtain product and technology synergy. In 1998 an agreement was signed with Malvern Instruments to expand sales of particle size analyzers.This alliance encompasses reciprocal distribution agreements and allows for future collaboration in the field of particle size analysis.