Quantitative PCR
REAL-TIME PCR SYSTEM
Real-Time PCR System
The first personal high-performance, low-cost real-time PCR system
With the Mx3000P™ system, we bring real-time PCR instrumentation to the personal market by offering a single system that provides high performance and full-feature design at a low cost. The instrument combines precision thermal control, a broad range optical system, and sophisticated software that is easy to learn and use. Most important, the Mx3000P system delivers outstanding results with all QPCR chemistries.
Optimized for Use with Stratagene’s Brilliant® QPCR and QRT-PCR Reagents
The Mx3000P system was designed for optimal performance with the use of our Brilliant® reagents. Brilliant QPCR and QRT-PCR reagents provide maximum versatility with high-quality, reproducible results. All of our reagent kits contain SureStart® Taq DNA polymerase, a hot-start version of Taq that minimizes amplification of non-specific PCR products. Whether you choose to optimize your reactions with a core reagent kit or prefer the convenience of a master mix, there is a Brilliant QPCR or QRT-PCR reagent kit for you.
Advanced Optical System Design
Four channels with customizable filters Extended excitation range supports all QPCR chemistries Unparalleled multiplex results using optimized illumination selection
Unsurpassed Real-Time PCR Results
Excellent uniformity and reproducibility Superior sensitivity with seven orders of magnitude Exceptional multiplex results with up to four targets
Most Powerful Software Available
Real-time data monitoring and evaluation Enhanced algorithms for precise data analysis Melting curve protocols for accurate product identification
Advanced Optical System for Ultimate Performance
Excitation filter wheel Halogen lamp Emission filter wheel
Coaxial fiber optic cable
1 blank filter position for calibration
Read head scans over each well, filter wheels rotate between scans
96-well plate
Unsurpassed Real-Time PCR Results
Excellent Uniformity
The thermal cycler in the Mx3000P system has outstanding thermal uniformity with only +/-0.25 ˚C variation in sample temperature across the 96-well plate. The temperature range of the thermal block is 25-95 ˚C. The thermal ramp rate is dynamically controlled by the software and can ramp up to 2.5 ˚C per second.
Outstanding Uniformity
SYBR® Green I uniformity run using a plasmid target and detection with target-specific primers and Stratagene’s Brilliant® SYBR Green Master Mix.
Exceptional Multiplexing
The Mx3000P system is specifically designed to ensure optimal results with multiplex assays using up to four targets in the same sample. The broad excitation range enables the use of dyes with maximal spectral separation. Emission from reporter dyes in the sample are collected by fiber optics, spectrally filtered, and detected by a photomultiplier tube (PMT). Narrow bandpass filters ensure finely delineated fluorescent signals and minimal noise. All of these components work together to provide extraordinary linear response for targets in singleplex and in multiplex.
A
Superior Sensitivity
The Mx3000P system achieves linearity of amplification over seven orders of magnitude, demonstrating the quality of fluorescence detection in the instrument. The Mx3000P system offers impressive discrimination with the ability to detect two-fold differences in initial template quantity with 99.7% confidence.
Enhanced Sensitivity
B Serial dilution of human genomic DNA. 5x107 to 50 genomic equivalents in 10-fold steps (n=3). Detection of ß-Actin using specific primers, a Cy5-labeled Molecular Beacon, and Stratagene’s Brilliant® QPCR Master Mix.
Unparalleled Multiplex Performance
Standard curves of four-fold serial dilutions of cDNA prepared from Stratagene’s QPCR Human Reference Total RNA, performed in singleplex (Panel A) and in multiplex (Panel B). The targets amplified were eNOS (FAM), HFE (HEX), CFTR (Texas Red®), and Cyclophilin (Cy5) as the endogenous reference.
Wide Linear Dynamic Range
The Mx3000P™ system demonstrates excellent resolution over seven orders of magnitude. The regression of correlation (r2) was 1.00 and the slope of the curve was –3.39.
Most Powerful Software Available
Easy Setup and Flexibility to Make Changes
The Mx3000P system software is so easy to use that you can learn the software and begin running experiments within minutes of installing your system. Plate setup and data analysis are fast and easy because your experiments are displayed in a 96-well format that matches the sample plate. The Quick Setup option saves you time by collecting data for all wells and all dyes—you can assign well information, reassign standards or change well destinations at your convenience—even while the experiment is running or after the run has finished.
Real-Time Results and Data Analysis
In addition to viewing the progress of your run in real time, you can also analyze your results as they are being generated. You have full access to raw data plots so you can assess PCR progress and elect to stop the experiment, saving valuable research time, or add cycles for further data collection and analysis.
Many Choices for Data Display and Analysis
You decide what data analysis format best suits your needs—amplification plots, scatter plots, melting curve profiles, fluorescent intensity values, and more. You can also decide how data will be analyzed: replicates may be treated individually or collectively, and data points for a given cycle can be used based on an average, minimum, maximum or last endpoint value.
Application Specific Analysis
Real-time allelic discrimination—calls by threshold cycle
or by endpoint fluorescence values
Molecular beacon melting curve—run and analyze
melting curves with automatic calculation of melting temperature or recommended annealing temperature range
Comparative quantitation—determine relative quantities
of target sample with respect to a calibrator using corrections for PCR efficiency of the gene of interest and the normalizer gene
SYBR ® Green I — verify
homogeneity of PCR products using dissociation curves
Fluorescence plate reads—use scatter plots,
fluorescence intensity values or final call results to identify presence or absence of product
Enhanced Algorithms for Standardized and Reproducible Results
The Mx3000P system uses enhanced algorithms such as replicate averaging, moving average, adaptive baseline, and amplification-based threshold to provide the most reliable and consistent data. You will always find outstanding reproducibility since these analysis algorithms are unchanging and applied to each data set.
Time-Saving Data Analysis
You can view all dyes on a single amplification plot without the need to export data. You can also run multiple experiments on the same plate and analyze single wells or groups of wells independently of others. In addition, you can easily export any graph or data file directly to PowerPoint, Excel, image, or text files, enabling you to create presentations and reports quickly and easily.
Mx3000P ™ Real-Time PCR System System Specifications
Excitation • Quartz Tungsten Halogen lamp • 350-750nm Detection • One scanning photomultiplier tube (PMT) • 350-700nm User Selected Filters • FAM™/SYBR® Green I • Texas Red®/ROX™ • TET • Cy5™ • HEX™/JOE™/VIC™ • Cy3™ • TAMRA™ • ALEXA Fluor® 350 Multiplexing capabilities • Up to four targets per sample Dynamic Range • 7 orders of magnitude Temperature Uniformity • +/- 0.25 ˚C Temperature Range • 25-95 ˚C Temperature Ramp Rate • up to 2.5 ˚C/second Sample Format • 200 µl, 96-well format optimized for 25 µl sample size Dimensions • 13” (33 cm) W x 18” (46 cm) D x 17” (43 cm) H Weight • 42 lbs (19 kg) Mx3000P Real-Time PCR Systems • 110 volt with notebook computer • 110 volt with desktop computer • 220 volt with notebook computer • 220 volt with desktop computer
Catalog#
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Practice of the patented polymerase chain reaction (PCR) process requires a license. The Mx3000P™ real-time PCR system is an Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. ALEXA Fluor® and Texas Red® are registered trademarks of Molecular Probes, Inc. CY™ is a trademark of Amersham Biosciences Limited
REAL-TIME PCR SYSTEM
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