SELF CONTAINED

                                200 to 100,000 GPD

       Aquapoint Inc. (USA)                    Aquapoint International
241 Duchaine Blvd. New Bedford, MA, 02745             Bioclere Systems Limited
    Phone: (508) 998-7577 (Sales ex: 3)                Phone: 0125-279-2688
           Fax: (508) 998-7177                          Fax: 0125-279-4068
       Email:                    Email:
                                                     Self contained
                                               Wastewater treatment systems

                                          THE BIOCLERE ADVANTAGE:
                                          Bioclere is a modified trickling filter over a clarifier. It
                                          is designed to treat wastewater with varying organic
                                          and nutrient concentrations as well as intermittent
                                          flows. Bioclere’s natural fixed film treatment process
                                          is stable, simple to maintain and inexpensive to

                                          Bioclere reduces biochemical oxygen demand
                                          (BOD5) and total suspended solids (TSS) to levels
                                          that meet or exceed NSF and EPA standards. As
    FEATURES & BENEFITS                   water trickles through the biofilter, organic material is
                                          consumed by a population of microorganisms that
Treats flows from 200 to 100,000 gpd      form on the surface of the media. Sloughed solids
                                          from the biofilter filter are returned to the primary
Cost effective treatment with efficient   tank as secondary sludge and treated water is
installation and operation                displaced to the next treatment component or the
                                          disposal area.
Treats high strength wastewater
                                          Bioclere is a modular technology. Units can be
Internal flow stabilization treats        installed in parallel to accommodate large flows or in
intermittent flows                        series to achieve high levels of treatment. The
                                          systems are sealed and insulated to minimize the
Fully automated pump system               impact of seasonal temperature variations on the
                                          treatment process.
Self adjusting process control

Small footprint / compact design          NITROGEN REDUCTION:
                                          Bioclere systems can be designed to consistently
Gravity flow system                       convert and reduce nitrogen. Total nitrogen is
                                          reduced substantially and cost effectively by
Quiet operation                           recirculation nitrified water from the Bioclere back to
                                          the primary settling tank. Large Bioclere systems
Sealed and insulated for seasonal         may incorporate a second stage nitrifying Bioclere
conditions                                and a tertiary anoxic reactor to achieve < 10 mg/l
                                          total nitrogen.
Durable UV resistant fiberglass
construction                              Applications include: residential, commercial,
                                          institutional, light industrial and municipal
Minimal energy usage                      wastewater treatment
Remote monitoring capabilities       

                                                                                               Typical Septic Tank Influent
                                                                                               Septic Tank Effluent
                                                                                               Bioclere Effluent

                                                                                              * Septic tank effluent is
SHOPPING CENTERS                                                                              diluted by the Bioclere's
                                                                                              recirculation system.

                                             Bioclere 16/12-350 is ANSI/NSF Standard 40 certified
                                             by the National Sanitation Foundation (NSF). The
                                             above performance results are based on a six month
COMMERCIAL CLUSTERS                          accumulative average from NSF’s certification testing

                                                                      U.S. Environmental Protection Agency’s (EPA)
                                                                      technology verification program. Results can be
                                                                      viewed at

                                             Standard Single Bioclere Installation:


 Represented by:

241 Duchaine Blvd. ● New Bedford, MA 02745
   Tel: 508-998-7577 ● Fax: 508-998-7177 ●          Technical Representation throughout the United States


      Bioclere is a modified trickling filter over a clarifier that was developed in Finland in the early
1960s and is being used throughout the United States and Europe for the secondary treatment of
wastewater. A biological fixed film treatment technology, Bioclere reduces biochemical oxygen
demand (BOD5) and total suspended solids (TSS) concentrations to levels that meet or exceed
National Sanitation Foundation (NSF) and Environmental Protection Agency (EPA) standards.
Bioclere’s treatment capabilities also include the conversion and reduction of nitrogen making it
ideal for nutrient sensitive sites. Aquapoint tertiary treatment products can be added to the process
chain to enhance nitrogen, phosphorus and turbidity reduction and to disinfect the waste stream.

           A 21,000 gpd Bioclere treatment system serving a housing development.
           Effluent requirements are <30 mg/l BOD, <30 mg/l TSS and <10 mg/l Total
           Nitrogen. A tertiary denitrifying ANOX Biological Reactor and UV Disinfection
           provide the final stages of treatment before effluent is distributed to a soil
           absorption field.


Treatment capacities from 200 to 100,000 gpd: A wide range of Bioclere filter sizes are available. The
modular units can be placed in parallel to accommodate large flows or in series to achieve high levels of

Broad range of applications: Bioclere installations include single family residences, residential clusters,
subdivisions, shopping malls, nursing homes, schools, restaurants, gas stations, golf courses, hotels,
medical facilities and municipalities.

Performance based engineered systems: Each system is sized by Aquapoint Engineers based on the
influent hydraulic and organic characteristics of your site. Specification of these variables is essential in
designing a Bioclere treatment system that will meet your permitted effluent requirements. A Design Criteria
form is included in this package for this purpose.

Fixed film treatment process: Naturally occurring
microorganisms attach themselves to the highly
permeable, randomly packed media and create a
biological filter. The wastewater is trickled through the
filter allowing organic matter to be absorbed by the
microorganisms. The filter is self-purging, self-regulating
and requires minimal maintenance. Bioclere utilizes
several different types of media varying in size, shape,
aperture size and surface area. Large media is generally
used for roughing applications and small media used for
more extensive treatment and polishing.
                                                                     Hufo Media
                                                                                              Flocor Media

Organic and hydraulic fluctuation: Generally Bioclere installations do not require flow equalization prior to
treatment. The ability of the biological fixed film to self-regulate over daily and seasonal variations in
hydraulic and organic loading, variations in temperature, pH and process inhibitors is widely acknowledged.

Bioclere 16/12-350 has been tested and proven by EPA’s Environmental Technology Verification (ETV)
program and is NSF standard 40 certified. NSF test results are included in this package on page (7) and
ETV results can be viewed at

Fully enclosed sturdy construction: Bioclere is constructed of insulated UV resistant fiberglass, PVC and
stainless steel. The systems are sealed and insulated making Bioclere ideal for extreme climate

Unaffected by short term power failures: Because the treatment process occurs above the gravity flow
line, electrical outages do not interrupt flow. Re-circulation of treated water within the system dilutes septic
tank effluent and minimizes the impact of short-term power failures on effluent quality.

Easy Retrofit: Bioclere can be easily installed between the septic tank and the distribution/disposal system
to upgrade treatment for existing facilities.

Low operating costs are characteristic of the trickling filter heritage. The simplicity of the design and the
stability of the fixed film process minimizes energy consumption and operation & maintenance costs
associated with wastewater treatment.

                                                            Minimal operation & maintenance requirements:
                                                            Routine maintenance procedures consist of
                                                            cleaning the spray distribution nozzles, and
                                                            ensuring proper functionality of the axial fan and
                                                            pumps. Aquapoint provides complete technical
                                                            manuals with all of our treatment systems. The
                                                            manuals include troubleshooting information,
                                                            maintenance checklists and other tools designed to
                                                            make operations & maintenance easy and effective.

                                                            Easy installation: Because Bioclere comes as a
                                                            pre-assembled modular system, units can be easily
                                                            installed with minimal field work. Aquapoint
                                                            provides onsite consultation for every Bioclere
       Bioclere dosing array spray nozzle maintenance       installation.

State of the art control panels: Bioclere control
panels are built in NEMA 4X watertight enclosures
allowing for indoor or outdoor installation. All control
panels utilize audio and visual alarms and can
incorporate remote dialers designed to call the
operator in the event of an alarm condition. Analog or
programmable logic control (PLC) panels are
available on all systems.

Remote monitoring capability: Bioclere controls
can be designed with Aquapoint’s Aqua Alert remote
wireless telemetry package. Aqua Alert provides the
operator with the ability to monitor system conditions
and manipulate system functions directly from an
internet accessible computer.                                   Bioclere PLC control panel with Aqua Alert telemetry package


    Wastewater flows from the septic or primary settling tank into a baffled chamber inside the Bioclere’s
clarifier. The baffled chamber prevents short circuiting of wastewater through the system. Alternating dosing
pumps are automatically activated by timers and periodically dose the media filter with wastewater from the
clarifier. Above the media filter a dosing array ensures uniform distribution of the wastewater over the media
surface. Oxygen is distributed throughout the filter by a fan that draws external air into the Bioclere.

   In the trickling filter the organic material in the wastewater is reduced by a population of microorganisms
that attach themselves to the media and form a biological slime layer known as biomass. Treatment is
accomplished in the outer layer of the biomass by aerobic microorganisms. As the microorganisms multiply
and the biological film thickens, diffused oxygen and organic substrate are consumed before the wastewater
penetrates the full depth of the film. Consequently, the biomass develops aerobic, anoxic and anaerobic

   As the microorganisms that are in contact with the plastic media become starved for oxygen and organic
carbon that is consumed by the surface layer, they lose their ability to cling to the media. The trickling
wastewater washes the biomass off of the media and a new biological film begins to form. This process of
losing the biomass is called “sloughing”. The rate of sloughing is primarily a function of organic and
hydraulic loading on the filter. This natural process does not compromise treatment and allows the media
bed to be self-purging, self-regulating and maintenance free.

  The sloughing biomass is washed through the trickling filter and settles to the bottom of the clarifier as
secondary sludge. Bioclere’s re-circulation system periodically pumps the secondary sludge back to the
septic tank where it is stored and eventually removed.

  This physical process is essentially the same for the reduction of BOD5 and nitrification (conversion of
ammonia nitrogen to nitrate nitrogen).

  Standard single Bioclere installation:


    Removing ammonia from wastewater is a well-established and quantifiable biological process. Nitrogen
exists in the influent waste stream primarily in the form of organic nitrogen and ammonia nitrogen (Total
Kejldahl Nitrogen or TKN). The principle part of the organic nitrogen is mineralized to ammonia nitrogen by
bacterial activity in the septic tank. Therefore, ammonia nitrogen is commonly regarded as the starting point
in the nitrogen reduction process.

Nitrification is the conversion of ammonia (NH3) nitrogen to nitrate (NO3) nitrogen. This biological process
is accomplished by Aerobic Autotrophs, Nitrosomonas and Nitrobacter bacteria in the presence of dissolved
oxygen. Bioclere effluent ammonia concentrations of 1 mg/l to 3 mg/l are reliably achieved.

   Successful nitrification is accomplished with a healthy microorganism population and an environment
where pH, alkalinity, temperature, organic loading and oxygen supply are relatively stable. In a Bioclere
system; the pH is buffered by the carbonate system associated with wastewater; the temperature remains
relatively constant because Bioclere provides an enclosed and insulated environment and the biomass
generates heat; the organic loading is relatively consistent because treated wastewater is recycled through
the septic tank and the Bioclere; and the fan provides an adequate supply of oxygen.

Denitrification is the conversion of nitrate (NO3) nitrogen, to nitrite (NO2) nitrogen and then to nitrogen gas
which is released into the atmosphere. This is a biological process performed by Facultative Heterotrophic
bacteria in the presence of a soluble carbon source and anoxic conditions (dissolved oxygen = < 0.3 mg/l).

   Denitrification occurs by several different means and through process control adjustments. In the
Bioclere trickling filter, diffused oxygen is used up by the aerobic outer portion of the biomass and anoxic
conditions are created within the biological film. This allows for significant nitrogen removal in the Bioclere
via simultaneous nitrification and denitrification. Denitrification is also achieved by re-circulating nitrified
wastewater from the Bioclere back to the septic tank where there is an anoxic zone and sufficient carbon in
the influent waste stream to denitrify. This process has achieved total nitrogen reductions up to 80 percent.


   Nitrogen removal can be enhanced further with an Aquapoint ANOX Biological Reactor or a denitrifying
sand filter following Bioclere treatment. Aquapoint employs tertiary denitrification when effluent total nitrogen
concentrations of <10 mg/l are required for sites with elevated influent TKN concentrations. These
denitrification methods have proven effective and are recognized as viable processes by the EPA.


    Bioclere treatment systems achieve phosphorus reduction by dosing the waste stream with coagulants
such as metal salts, typically aluminum or iron. This type of phosphorus removal is simple and is the most
commonly used method in the United States. It consists of adding metal salts that react with phosphates in
the wastewater to form insoluble precipitates. The precipitates settle to the bottom of the treatment chamber
reducing the phosphorus concentrations in the wastewater. Coagulant dosing rates are based on the
stoichiometric metal salt to phosphorous ratio dictated by the concentration of phosphorus in the daily
wastewater flow. The efficiency of phosphorus removal is simply related to the coagulant dose provided that
alkalinity is present in sufficient quantities. The precipitated sludge can be processed in the same manner
as typical settled sludge. This type of phosphorus reduction can effectively achieve 80-95 percent total
phosphorus removal and effluent concentrations of <1 mg/l. Greater reduction in total phosphorus can be
achieved by incorporating a physical barrier filter such as a sand filter, disc filter or a membrane.


NOTES: The median is the point where half of the values are greater and half are less

        The interquartile range is the range of values about the median between the upper and lower 25% of all

   Testing of the Aquapoint Bioclere model 16/12-350 was conducted under the provisions of ANSI/NSF
Standard 40 for Residential Wastewater Treatment Systems (January 1999 revisions). ANSI/NSF
Standard 40 was developed by the NSF Joint Committee on Wastewater Technology.

   The performance evaluation was conducted at the Buzzard’s Bay Project Test Center located on the
Otis Air National Guard Base in Massachusetts using residential wastewater diverted from the base. The
evaluation started with two weeks of dosing without sampling to allow for plant start-up. Once a treatment
biomass was established, NSF sampled for sixteen weeks at design flow, performed seven weeks of
stress testing and then sampled for three more weeks at designed flow. Sampling began in late summer
and continued into the winter, covering a range of operating temperatures.

   Over the course of the evaluation, the average effluent CBOD5 was 11 mg/l, ranging between 4 and 26
mg/l. The average effluent TSS was 5 mg/l, ranging between <2 and 15 mg/l. Effluent pH ranged from 6.1
to 8.1 within the required range of 6.0 to 9.0. The plant also met the requirements for noise levels (less
than 60 dbA at a distance of 20 feet), color, threshold odor, oil film and foam.


The septic tank or primary settling tank performs the primary treatment stage of the Bioclere process. Sizing
of the primary tank should take into consideration the effect of re-circulation on detention time.

Bioclere units are constructed with the effluent pipe 180 degrees opposite the influent pipe.

The only routine service procedures required are pumping of the primary tank, Bioclere pump and fan
maintenance, and infrequent cleaning of the dosing array nozzles. Tertiary treatment equipment added to
the process chain will require additional service. In most states a licensed wastewater treatment plant
operator is required to perform this maintenance.

The pre-assembled, self-contained Bioclere units are easily installed into new or existing facilities.
Aquapoint’ s operations team will supply a Bioclere lifting harness and will provide onsite consultation for
every Bioclere installation. Concrete pads with mounting eyes are supplied by the contractor and set at the
appropriate elevations before the Bioclere is set on the pad and chained to the mounting eyes. The Bioclere
is leveled by adjusting turnbuckles on the chains. Interconnecting piping between the septic tank, Biocleres
and additional treatment or disposal equipment is the responsibility of the contractor. Multiple units can be
installed in a few hours with adequate site preparation.

The Bioclere is commissioned by filling the clarifier with fresh water so that the dosing and re-circulation
systems can be tested. Once the Bioclere is commissioned it is ready to receive wastewater flow and will
take four to eight weeks to establish a healthy biomass for treatment.

            A 7,250 gpd Bioclere system serving a twenty four home residential cluster. Effluent
            requirements are <30 mg/l BOD, <30 mg/l TSS, <5 mg/l Ammonia and <19 mg/l Total
            Nitrogen. Bioclere effluent is pressure dosed to a soil absorption field for disposal.

                                                                                                               Design Criteria 1/16/06
                                                                                                               Tel: (508) 998-7577
                                                                                                               Fax: (508) 998-7177
Date:                                           SYSTEM DESIGN CRITERIA                         Requested Response Time:

Engineer:                                                                          Customer/Contact:
Address:                                                                           Project Name:
Tel:                                     Fax:

Application / Description:                Residential       Commercial          Industrial    Institutional      School       Other
Provide relevant details:

Type of Collection System:                Gravity           Grinder Pumps       S.T.E.P.      Pump Stations      S.T.E.G      Other

Type of Disposal System:                  Stream            Conventional Leach Field          Drip Disposal      Spray        Other

Wastewater Flow Calculations:                                                      Comments: (topography, permits, flow calculations,
(Please show calculations that are basis of the design).                           technology requests, containerization requests, etc.
                                                                                   Please append information as needed.)
Design Flow:
Actual Daily Flow:
Maximum Daily Flow:
Peak Hourly Flow or peaking factor (please specify):

Residential, Municipal, Commercial or Industrial Applications*
* Please obtain composite samples at the septic tank effluent tee for the parameters listed under INFLUENT DATA
* Please provide Aquapoint with all applicable Material Safety Data Sheets (MSDS)
                          INFLUENT DATA                                                             EFFLUENT REQUIREMENTS
Specify sample type & location:     Grab Sample           Composite Sample         Please specify location of effluent requirements:
  Septic tank influent                     Septic tank effluent                    e.g. System effluent pipe; groundwater interface; property line
Seasonal Flows:       Y       N when?
Will low flow devices be utilized?:    Y     N                                     Please specify CBOD5 for organic load when allowed
Will garbage disposal/grinders be utilized?:   Y N                                 pH:
Wastewater Temperature (10o C assumed unless specified):                           CBOD5:
Site Elevation (feet above sea level):                                             BOD5:
Parameter                                  Raw           Settled                   COD:
pH:                                                                                TSS:
BOD5:                                                                              Ammonia-N:
COD:                                                                   Nitrate-N:
TSS:                                                                   Total Nitrogen:
TKN:                                                                   Oil & Grease:
Alkalinity:                                                            Phosphorus:
Ammonia-N:                                                             Fecal Coliform:
Oil & Grease (are traps included?):                                    Dissolved Oxygen:
Phosphorus:                                                            Turbidity:
Other:                                                                 Other:
NOTES: * Commercial installations require baffled septic tanks and a gas baffle under the effluent tee

Aquapoint Representative:                               Date:                      Engineer Signature:                                  Date:

IMPORTANT: The information provided by you on this form will be relied upon by Aquapoint Inc. in determining project
specifications and pricing. Accordingly, all such information must be complete and accurate and any fact or circumstance
that comes to your attention after the date hereof that may in any way render any information contained herein incomplete or
inacurate must be brought to the attention of Aquapoint Inc. immediately.

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