Tissue-Specific Defects Are Caused by Loss of the Drosophila MAN1 LEM Domain Protein

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Tissue-Specific Defects Are Caused by Loss of the Drosophila MAN1 LEM Domain Protein Powered By Docstoc
					Copyright Ó 2008 by the Genetics Society of America
DOI: 10.1534/genetics.108.091371



       Tissue-Specific Defects Are Caused by Loss of the Drosophila MAN1
                              LEM Domain Protein

      Belinda S. Pinto,* Shameika R. Wilmington,† Emma E. L. Hornick,† Lori L. Wallrath*,†
                                    and Pamela K. Geyer*,†,1
*Molecular and Cellular Biology Program and †Department of Biochemistry, University of Iowa, College of Medicine, Iowa City, Iowa 52242
                                                       Manuscript received May 14, 2008
                                                      Accepted for publication July 6, 2008


                                                              ABSTRACT
                The nuclear lamina represents a protein network required for nuclear structure and function. One family
             of lamina pro
				
DOCUMENT INFO
Description: The nuclear lamina represents a protein network required for nuclear structure and function. One family of lamina proteins is defined by an ~40-aa LAP2, Emerin, and MAN1 (LEM) domain (LEM-D) that binds the nonspecific DNA-binding protein, barrier-to-autointegration factor (BAF). Through interactions with BAF, LEM-D proteins serve as a bridge between chromosomes and the nuclear envelope. Mutations in genes encoding LEM-D proteins cause human laminopathies that are associated with tissue-restricted pathologies. Drosophila has five genes that encode proteins with LEM homology. Using yeast two-hybrid analyses, we demonstrate that four encode proteins that bind Drosophila (d)BAF. In addition to dBAF,dMAN1 associates with lamins, the LEM-D protein Bocksbeutel, and the receptor-regulated Smads, demonstrating parallel protein interactions with vertebrate homologs. P-element mobilization was used to generate null dMAN1 alleles. These mutants showed decreased viability, with surviving adults displaying male sterility, decreased female fertility, wing patterning and positioning defects, flightlessness, and locomotion difficulties that became more severe with age. Increased phospho-Smad staining in dMAN1 mutant wing discs is consistent with a role in transforming growth factor (TGF)-β/bone morphogenic protein (BMP) signaling. The tissue-specific, age-enhanced dMAN1 mutant phenotypes are reminiscent of human laminopathies, suggesting that studies in Drosophila will provide insights into lamina dysfunction associated with disease. [PUBLICATION ABSTRACT]
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