; Functional Characterization of the dRYBP Gene in Drosophila
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Functional Characterization of the dRYBP Gene in Drosophila

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The Drosophila dRYBP gene has been described to function as a Polycomb-dependent transcriptional repressor. To determine the in vivo function of the dRYBP gene, we have generated mutations and analyzed the associated phenotypes. Homozygous null mutants die progressively throughout development and present phenotypes variable both in their penetrance and in their expressivity, including disrupted oogenesis, a disorganized pattern of the syncytial nuclear divisions, defects in pattern formation, and decreased wing size. Although dRYBP mutations do not show the homeotic-like phenotypes typical of mutations in the PcG and trxG genes, they enhance the phenotypes of mutations of either the Sex comb extra gene (PcG) or the trithorax gene (trxG). Finally, the dRYBP protein interacts physically with the Sex comb extra and the Pleiohomeotic proteins, and the homeotic-like phenotypes produced by the high levels of the dRYBP protein are mediated through its C-terminal domain. Our results indicate that the dRYBP gene functions in the control of cell identity together with the PcG/trxG proteins. Furthermore, they also indicate that dRYBP participates in the control of cell proliferation and cell differentiation and we propose that its functional requirement may well depend on the robustness of the animal. [PUBLICATION ABSTRACT]

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									Copyright Ó 2008 by the Genetics Society of America
DOI: 10.1534/genetics.107.082966



              Functional Characterization of the dRYBP Gene in Drosophila

                                   Inma Gonzalez, Ricardo Aparicio and Ana Busturia1
                                            ´
              Centro de Biologıa Molecular CSIC-UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
                              ´                                     ´
                                                      Manuscript received October 9, 2007
                                                      Accepted for publication May 6, 2008


                                                             ABSTRACT
                 The Drosophila dRYBP gene has been described to function as a Polycomb-dependent transcriptional
              repressor. To determine the in vivo function of the dRYBP gene, we have generated mutations and
              analyzed the associated phenotypes. Homozygous null mutants die progressively throughout development

								
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