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Selectivity and Action of White-Rot Fungus Ceriporiopsis


									                                         Poster Presentation 3B-61

  Selectivity and Action of White-Rot Fungus Ceriporiopsis subvermispora on
                   Sugarcane Bagasse: 20-L Fermentation.

                       Sirlene M. Costa1, Adilson R. Gonçalves*1 and Elisa Esposito2
        Depto. Biotecnologia-FAENQUIL, Cx. Postal 116, CEP 12.606-000, Lorena - SP, Brazil.
                      Phone: 12 3159-5007 Email:
               Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Brazil.

In this work the action of the white-rot fungus Ceriporiopsis subvermispora in the fibers of the sugarcane
was monitored by periods of time from 7 to 60 days using varied inoculum charge (100, 250, 500 and 750
mg of fungi per kg of bagasse). The solid state fermentation of 800 g bagasse was carried out in 20-L
bioreactors. The action fungal of Ceriporiopsis subvermispora was evaluated by chemical analysis and
also by enzymatic activities measured as lignin peroxidase (LiP), manganese peroxidase (MnP), laccase
(Lac) and xylanase (Xyl). Table 1 shows the results of losses of mass and components of bagasse samples
treated with 250 mg/kg and also that the C. subvermispora was selective for the degradation of lignin.
The results of enzymatic activities showed that after 30 day of C. subvermispora incubation in the fibers
the values of Xyl activities were in the range of 4500 UI/kg independently of the inoculum charge. The
MnP has a maximum of production until 30 days and Lac and LiP were not detected.

                                                        Biodegradation time (days)
                  Losses (%) / selectivity      7         15      30         45            60
                  Mass                         10         12     13.3       17.1          19.1
                  Xylan                       11.2        7.9     6.9       12.1          13.4
                  Glucan                      9.7        12.9    17.7       25.4          24.7
                  Lignin                       19        22.1    25.6       21.6          33.1
                  Selectivity                 1.7         2.8     3.8        1.8          2.5

           “ Selectivity is the loss of lignin / loss of glucan; values are related to control

[Financial support from Fapesp, CAPES and CNPq - Brazil]

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