FY 2010 Contagious Equine Metritis (CEM) National Testing Project

Document Sample
FY 2010 Contagious Equine Metritis (CEM) National Testing Project Powered By Docstoc
					             FY 2010
    Contagious Equine Metritis
              (CEM)
      National Testing Project

Manual for Active Breeding Stallions

           January 2010
Contents


1. Testing Protocol for Active Breeding Stallions ............................................... 1-1
2. Laboratory Submission and Reporting Protocol for Active Breeding
    Stallions.......................................................................................................... 2-1
           Appendix 1 – Approved CEM Laboratories ......................................... A1-1
           Appendix 2 – Example of VS Form 10-4 Specimen Submission......... A2-2
3. Stallion Reproductive Anatomical Photographs ............................................. 3-1
    Courtesy of Dr. Michaela Kristula, University of Pennsylvania
                       USDA APHIS VETERINARY SERVICES

                                    FY 2010
                          CONTAGIOUS EQUINE METRITIS
                           NATIONAL TESTING PROJECT

       TESTING PROTOCOL FOR ACTIVE BREEDING STALLIONS

                                          January 2010

I.     General Information

The purpose of this protocol is to provide guidelines for testing active breeding stallions as part
of the FY 2010 Contagious Equine Metritis (CEM) National Testing Project. CEM is a venereal
disease of horses caused by Taylorella equigenitalis.

Beginning in February 2010, swab samples will be collected from 3,000 active breeding stallions
from around the United States and tested for T. equigenitalis. Active breeding stallions are
considered to be those that had semen collected, or were bred to a mare by live cover, in 2008 or
2009.

Specific sampling targets have been set for each State in order to produce the overall national
sampling total of 3,000 breeding stallions. The number of stallions to be sampled from each State
is based on APHIS National Animal Health Monitoring System estimates of breeding stallion
populations in each State and on the number of equine practitioners indicating a reproduction
focus to their practice in each State as compiled in the directory of the American Association of
Equine Practitioners (see Table 1 for State sampling targets). For purposes of this testing project,
the State of sampling is considered to be the State in which the stallion is standing at the time of
sampling, regardless of the usual State of residence of the stallion or its owner.

Enrollment of stallions in the national testing will be voluntary in all cases; for information on
the potential benefits of enrollment and how to volunteer, see section VI, Enrollment. Sample
collection will be performed by an accredited equine veterinarian. Stallion owners are required to
pay the accredited equine practitioner for sample collection costs.

No quarantine restrictions need to be imposed at the time of sample collection or while test
results are pending. If a stallion is found positive, the positive stallion must be placed under State
quarantine or hold order until he is treated, retested, and determined to no longer be infected with
T. equigenitalis. APHIS will pay all costs associated with the necessary treatment and retesting
procedures of stallions that test positive in this national testing project.

No Federal or State regulatory oversight of sample collection is required. However, any State
animal health authority that desires to have oversight can make it a requirement. Extra costs
incurred from that oversight will be at State expense.


                                                 1-1
                CEM National Testing Project – Protocol for Active Breeding Stallions
                                          January 2010

Sampling will consist of one set of swabs for culture. One set includes three swabs: one swab
collected from the fossa glandis, one from the urethral sinus, and one from the distal urethra. No
post-testing antibiotic treatment is required (unless the stallion is found positive).

Diagnostic testing will be performed only at APHIS-approved CEM laboratories or at the
National Veterinary Services Laboratories (NVSL). All suspect positive cultures must be sent to
the NVSL for confirmation.

APHIS will pay the laboratory diagnostic testing costs for one set of swabs, including shipment
of samples to an approved CEM laboratory or to the NVSL. Collection, shipping, and testing of
any additional swab sets are at the stallion owner’s discretion and expense.

T. equigenitalis is a fastidious gram-negative coccobacillus. Adherence to sampling and
submission protocols is necessary to reduce the risk of false negative results. Cultures must be
placed in Amies transport media with charcoal and shipped refrigerated (e.g., with ice packs).
Samples must be received and plated at an APHIS-approved CEM laboratory or at the NVSL
within 48 hours of collection.

Stallions should not be treated with antibiotics while undergoing T. equigenitalis testing. If a
stallion requires systemic antibiotic treatment, then a minimum of 7 days must elapse between
the last systemic treatment and any sample collection. If a stallion requires topical treatment of
the penis or prepuce with an antibiotic, antiseptic, or antibacterial of any kind, then a minimum
of 21 days must elapse between the last topical treatment and the collection of a sample for
T. equigenitalis culture.

No cleaning of the external genitalia should take place prior to testing except as described in
Section III of this document, since cleaning of the external genitalia prior to or during culturing
can lead to false negative results.

II.    Eligibility of Active Breeding Stallions for Testing

Active breeding stallions are considered to be those that had semen collected, or were bred to a
mare by live cover, in 2008 or 2009. However, some active breeding stallions are not eligible for
enrollment in the national testing project. Stallions that are not eligible for testing include:
• Stallions known to have been exposed to a positive horse in the current CEM outbreak.
• Stallions tested for T. equigenitalis in the previous 6 months.
• Stallions that received any systemic antibiotic treatment in the previous 7 days, or any topical
   treatment of the penis or prepuce with an antibiotic, antiseptic, or antibacterial of any kind in
   the previous 21 days. (These stallions could be eligible for testing, given they meet other
   eligibility requirements, if they are sampled after an appropriate waiting period following
   treatment (i.e., 7 to 21 days).)

In addition, the maximum number of stallions to be sampled at any one premises or facility will
be limited to 50 percent of the total number of active breeding stallions present, but not more
than 20 stallions in total. Sampling of more than 20 stallions at a given facility or premises may
be approved by APHIS in some situations.


                                                1-2
                CEM National Testing Project – Protocol for Active Breeding Stallions
                                          January 2010

Of the active breeding stallions otherwise eligible for testing, those of highest priority for
enrollment are:
• Stallions collected or bred at a facility where at least one other stallion was collected or bred
    on the same day in 2008 or in 2009;
• Stallions collected or bred on two or more days in 2008 or in 2009;
• Stallions collected or bred in both 2008 and 2009;
• Stallions potentially collected or bred outside the United States at any time.

After using the priority categories to target stallions for enrollment, some attention should be
given to their breed. To the extent possible, participating stallions in any given State should not
all be of the same breed, but should generally reflect the breeds found in that State’s overall
breeding stallion population.

III.   Collecting Samples from Active Breeding Stallions

Sample collection will be performed only by a USDA-accredited equine veterinarian. In addition
to accreditation, State animal health officials may require sample collection training or
experience for equine practitioners collecting samples in their jurisdiction.

Accredited equine veterinarians must have prior approval from the APHIS-VS Area Veterinarian
in Charge (AVIC), or from the State Animal Health Official, of the State in which sampling will
be performed. The stallion owner is responsible for the cost of sample collection.

APHIS will pay for the shipping and laboratory diagnostic testing of one set of swabs per eligible
breeding stallion. One set includes three swabs: fossa glandis, urethral sinus, and distal urethra.
Collection, shipping, and testing of any additional swab sets are at the stallion owner’s discretion
and expense.

Samples must only be collected on a Monday, Tuesday, Wednesday, or Thursday, in order to
allow samples to reach the diagnostic laboratory on a weekday via overnight delivery. Also,
samples should not be collected on any day immediately prior to a holiday. Samples must be
plated within 48 hours of collection and laboratories will not authorize overtime work (e.g.,
weekends, holidays) for this national testing project.

Culture the urethral sinus, the fossa glandis, and the distal urethra, using a separate swab for each
of the three sites. Swab sites may be cleaned with cotton and plain water to remove excess
organic debris, but no residual water should be present at the time of swabbing. Do not scrub or
use an antiseptic or antibacterial of any kind prior to swabbing. If an antiseptic or antibacterial
has been used, stallions must wait 21 days to have samples collected for T. equigenitalis culture.

The accredited equine veterinarian collecting the samples must fax a copy of the VS Form 10-4
for each stallion sampled to the AVIC for the State in which the samples were collected.




                                                1-3
                CEM National Testing Project – Protocol for Active Breeding Stallions
                                          January 2010

IV.    Stallions Found Positive for T. equigenitalis

A stallion is positive for T. equigenitalis if positive results are returned from the NVSL on any of
the testing performed. A positive stallion must be placed under a State quarantine or hold order
until the stallion is treated, retested, and determined to no longer be infected with
T. equigenitalis.

Stored (frozen) semen collected from a stallion found positive for T. equigenitalis should be
quarantined, or otherwise held without use, pending an epidemiological determination of the year
of infection of the donor horse. Semen collected during or after the year of initial T. equigenitalis
exposure may pose a risk for exposure to recipient horses.

Stallions confirmed as positive at the NVSL will be re-sampled and cultured at the NVSL, at
APHIS expense, to reconfirm the status of the stallion to be treated. Procedures for treating a
positive stallion are detailed in Section IV of the “USDA APHIS Veterinary Services FY 2009
CEM Incident Testing Protocol for Investigations.”

APHIS will pay all costs associated with the necessary treatment and retesting procedures of
stallions that test positive in this national testing project. However, cost sharing and procedures
for associated tracing and testing/treatment of horses exposed to a positive stallion will be the
same as in the current outbreak response (see “FY 2009 CEM Incident Manual”). APHIS and
State animal health authorities share the cost of tracing activities. APHIS pays for laboratory
diagnostics and shipping of samples, but not for sample collection, test mares, or treatment for
exposed horses. Owners of the exposed horses are responsible for the cost of sample collection,
test mares, or treatment. If any exposed horses are found positive, those horse owners are
responsible the cost of treatment and retesting of that horse until it is determined to be negative.

V.     Sample Collection and Submission

       1. Animal health officials and accredited veterinarians must refer to the “Laboratory
          Submission and Reporting Protocol for Active Breeding Stallions” for specific
          sample collection and submission guidance.

       2. General guidance:

           a. Active breeding stallion samples are to be sent to an approved CEM laboratory or
              to the NVSL. All suspect positive samples must be confirmed at the NVSL.

           b. Swabs must be placed in Amies transport medium with charcoal (each swab in a
              single tube) and refrigerated. (Amies media is available at no charge through VS
              Area Offices.)

           c. The swabs that come in the Amies tubes are adequate for collection of stallion
              samples.




                                                 1-4
                CEM National Testing Project – Protocol for Active Breeding Stallions
                                          January 2010

           d. VS Form 10-4 must be used for all submissions, whether to an approved CEM
              laboratory or to the NVSL. A separate 10-4 should be used for each stallion
              sampled. Required forms and specimens should be identified with the name,
              address, and phone/fax number of submitting veterinarian; location of stallion;
              complete animal identification; anatomical locations sampled; and date and time
              of sample collection. (See Appendix 2 of the “Laboratory Submission and
              Reporting Protocol for Active Breeding Stallions”.)

           e. Swabs must be shipped with ice packs, by an overnight service provider as
              identified by the VS Area Office or VS Regional Office, to an APHIS-approved
              CEM laboratory in the State of sample collection (see Appendix 1, Approved
              CEM Laboratories). If no CEM laboratory exists in the State of sample collection,
              samples may be shipped to an APHIS-approved CEM laboratory in another State
              or to the NVSL (shipping address: National Veterinary Services Laboratories,
              Diagnostic Bacteriology Laboratory (DBL), 1920 Dayton Ave., Ames, IA 50010).
              Prior to submission of culture samples to the NVSL, contact Alan Aalsburg (515-
              337-7957) or Dr. Matt Erdman (515-337-7565 or cell: 515-231-4147).

           f. All samples must reach the lab and be plated within 48 hours after collection. If
              swabs are delayed, the sample collection must be repeated.

           g. Incubation for T. equigenitalis takes a minimum of 7 days.

           h. For suspect positive samples being shipped from a CEM laboratory to the NVSL
              for confirmation, fax the original VS Form 10-4 from the submission to the
              approved CEM laboratory and the 10-4 submission for confirmation testing to
              515-663-7043. Include both 10-4 forms in with the samples when shipped.

           i. A swab of suspect colonies should be sent to the NVSL in Amies media with
              charcoal. If suspect colonies are noted on multiple anatomical locations from the
              same set of swabs, send a suspect colony from each location in individual Amies
              media. The original swabs do not need to be sent to NVSL.

           j. Web link to list of laboratories approved by APHIS to process CEM
              (T. equigenitalis) cultures:
              http://www.aphis.usda.gov/animal_health/lab_info_services/downloads/ApprovedLabs_CEM.pdf

VI.    Enrollment

Enrollment in the national testing project has at least three potential benefits to the owner of an
active breeding stallion. First, this is an opportunity to determine that a stallion likely does not
have the bacterium that causes CEM and that he is not spreading it to other horses. Second, a
breeding stallion owner may need a negative test result for a stallion in order to have him
collected or bred at a commercial facility this year. If so, enrollment offers a lower cost way (i.e.,
with no sample shipping costs or laboratory diagnostic costs) to get testing that may meet that
need. Third, if an owner has any reason to suspect that a stallion is positive, then this is a chance


                                                 1-5
                CEM National Testing Project – Protocol for Active Breeding Stallions
                                          January 2010

to find out with minimal financial impact. Any stallion found positive as part of this national
testing effort will be treated and retested at USDA’s expense. If they wait, however, and he is
found positive in the future, for example, as part of an investigation of another CEM-positive
horse, the positive stallion’s owner will be responsible for the cost of treatment and retesting of
the stallion, including the cost of test mares needed to confirm a negative status after treatment.

Stallion owners or accredited equine practitioners interested in participating should contact the
VS Area Office or the State Animal Health Official’s office to volunteer. Contact information for
your nearest office can be found on the following web pages:

               http://www.aphis.usda.gov/animal_health/area_offices/
               http://www.aphis.usda.gov/vs/sregs/official.html

         Table 1. Number of Active Breeding Stallions to be Sampled in Each State
           Eastern Region        Stallions to be         Western Region     Stallions to be
               States              Sampled                   States           Sampled
        Alabama                       48             Alaska                        0
        Connecticut                    10            Arizona                       55
        Delaware                        5            Arkansas                      32
        Florida                       133            California                   187
        Georgia                        35            Colorado                      72
        Illinois                      59             Hawaii                        0
        Indiana                        77            Idaho                        63
        Kentucky                      333            Iowa                          50
        Maine                          6             Kansas                       44
        Maryland                       32            Louisiana                     44
        Massachusetts                  16            Missouri                     82
        Michigan                      63             Montana                      53
        Minnesota                      62            Nebraska                      38
        Mississippi                    40            Nevada                        21
        New Hampshire                   2            New Mexico                   39
        New Jersey                     35            North Dakota                  17
        New York                      102            Oklahoma                     94
        North Carolina                 49            Oregon                        62
        Ohio                          107            South Dakota                  27
        Pennsylvania                  101            Texas                        327
        Rhode Island                    1            Utah                          28
        South Carolina                 36            Washington                    61
        Tennessee                      91            Wyoming                       22
        Vermont                         4                    Total               1,418
        Virginia                      65
        West Virginia                  17
        Wisconsin                      53
                 Total               1,582



                                                   1-6
                        USDA APHIS VETERINARY SERVICES

                                     FY 2010
                           CONTAGIOUS EQUINE METRITIS
                            NATIONAL TESTING PROJECT

                 LABORATORY SUBMISSION AND REPORTING
                PROTOCOL FOR ACTIVE BREEDING STALLIONS

                    APPROVED CEM LABORATORIES
                              AND THE
             NATIONAL VETERINARY SERVICES LABORATORIES

                                          January 2010

I.        General Information
The National Veterinary Services Laboratories (NVSL) VS Form 10-4 will be used for
submitting all samples collected from active breeding stallions and submitted to either an
APHIS-approved contagious equine metritis (CEM) laboratory or to the NVSL.

Culture Submissions.

     1. There are 15 APHIS-approved CEM laboratories for bacteriological cultures of
        Taylorella equigenitalis as trained by the NVSL and following NVSL standard operating
        procedures (i.e., SOPTA-BACT-0001.07). A list of approved CEM laboratories,
        including addresses for sample shipment, can be found in Appendix 1.

     2. All submissions for culture must go to an approved CEM laboratory or to the NVSL.

     3. Samples collected in a State with an approved CEM laboratory should be submitted to
        that laboratory, unless the submitter is instructed otherwise. If no approved CEM
        laboratory exists in the State of sample collection, samples may be shipped to an APHIS-
        approved CEM laboratory in another State or to the NVSL.

     4.   VS Form 10-4 must be used for all submissions, whether to an approved CEM laboratory
          or to the NVSL. A separate 10-4 should be used for each stallion sampled; see Appendix
          2 for an example of a specimen submission form for active breeding stallions. (A fillable
          version of VS Form 10-4, specifically for use with active breeding stallion submissions
          for the CEM national testing project, may be accessed at:
          http://www.aphis.usda.gov/animal_health/lab_info_services/downloads/VS_Form10-
          4_CEM-SURV.pdf.)




                                                 2-1
               Laboratory Submission and Reporting Protocol for Active Breeding Stallions
                             Approved CEM Laboratories and the NVSL
                                            January 2010

      5. The accredited equine veterinarian collecting the samples must fax a copy of the VS
         Form 10-4 for each stallion sampled to the AVIC for the State in which the samples were
         collected.

      6. The VS Form 10-4 for each stallion sample submission to an approved CEM laboratory
         must be faxed to the NVSL (515-663-7043) by the approved CEM laboratory within a
         day or two of receipt.

      7. All suspect positive colonies found at an approved CEM laboratory must be submitted
         immediately to the NVSL for confirmation.

      8. The correct address for submitting culture samples to the NVSL:

                 National Veterinary Services Laboratories (NVSL)
                 Diagnostic Bacteriology Laboratory (DBL)
                 1920 Dayton Ave., Ames, IA 50010

      9. Prior to submission of culture samples to the NVSL, contact Alan Aalsburg (515-337-
         7957 or 515-520-2114) or Dr. Matt Erdman (515-337-7565 or cell: 515-231-4147).

      10. In addition, fax the original 10-4 from the submission to the approved CEM laboratory
          and the new 10-4 for confirmation testing to 515-663-7043. Include both 10-4 forms in
          with the samples when shipped.

II.      Submission Requirements for Approved CEM Laboratories for Culture
      1. The State or Federal animal health official authorizing the submission of samples must
         contact the selected approved CEM laboratory prior to shipping the samples to ensure
         testing capacity is available and to confirm any specific submission requirements for the
         laboratory (e.g., notification required). Points of contact for approved CEM laboratories
         and their addresses are provided in Appendix 1.

      2. Samples for culture must be shipped to reach the lab and be plated within 48 hours after
         collection or they will not be tested. Overtime, weekend and holiday work is not expected
         at approved CEM laboratories or at the NVSL, so plan accordingly in the collection and
         shipment of samples.

      3. Sample collection:

         a. Culture swabs must be placed in Amies transport medium with charcoal (one swab
            per tube) and refrigerated. (This transport media is provided at no cost by the NVSL
            through APHIS-VS Area Offices; the contact for Area Offices to obtain this media is
            Alan Aalsburg (515-337-7957 or 515-337-7565).)

         b. The swabs that come in the Amies tubes are adequate for collection of stallion
            samples.



                                                  2-2
            Laboratory Submission and Reporting Protocol for Active Breeding Stallions
                          Approved CEM Laboratories and the NVSL
                                         January 2010

       c. Specimens should be identified with the name, address and phone/fax number of
          owner and submitting veterinarian (State or Federal animal health official); location
          of horse; complete animal identification; anatomical location of each swab; and date
          and time of collection.
          Note that the minimum information to be written on each tube is sample ID number,
          horse number or name, and anatomical location, since space on tube is limited.

       d. Swabs must be shipped with ice packs, by an overnight service provider as identified
          by the VS Area Office or VS Regional Office, to an approved CEM laboratory in the
          State of sample collection.

       e. Requests for shipping boxes should be directed to:

              NVSL Shipping
              Fax Number: 515-663-7378
              Email: NVSLShipping@aphis.usda.gov
              Phone Number: 515-337-7530 (prefer fax or email orders if possible)

       f. The accredited equine veterinarian collecting the samples must fax a copy of the VS
          Form 10-4 for each stallion sampled to the AVIC for the State in which the samples
          were collected.

       g. Incubation for T. equigenitalis takes a minimum of 7 days.

III.   VS Form 10-4 Requirements for Culture Submission
  Submissions from Active Breeding Stallions – Submit to an approved CEM laboratory
  or to the NVSL:
  A separate 10-4 should be used for each stallion sampled; see Appendix 2 for an example of a
  specimen submission form for active breeding stallions.
   Box 1    Name of Submitter – The State or Federal animal health official authorizing the
            sample submission; include email address of submitter in the mailing address
            section in addition to the mailing address, phone and fax.
   Box 4    Location of Animals – Enter location of stallion at time of sampling, including
            premises ID number, if applicable.
   Box 5    Payment Method – Leave blank.
   Box 9    Examinations Requested – Enter “CEM Culture” and record the time of sample
            collection.
   Box 10 Collected by – The accredited veterinarian who actually collected the sample.
   Box 12 Authorized by – AVIC of submitting State.
   Box 13 Purpose of Submission – Check “Surveillance.”




                                               2-3
              Laboratory Submission and Reporting Protocol for Active Breeding Stallions
                            Approved CEM Laboratories and the NVSL
                                           January 2010

     Box 15 Referral Number – Must contain the reference “CEM-SURV;” add any referral
            number or local reference number, as needed.
     Box 21 Identification – List each sample collection site separately under “Sample ID” –
            collection sites (one swab each) are: distal urethra, urethral sinus, and fossa
            glandis; under “Animal ID” give the name of the stallion, in addition to any other
            names or identification available; indicate breed, age, and sex in the appropriate
            columns.
     Box 22 Additional Data – Record the exact registered name, or other full name, of the
            breeding stallion, plus his breed registration number (if applicable). Indicate “CEM
            surveillance stallion.” Indicate priority category(ies) of stallion sampled:
            (1) collected/bred with other stallion(s) on same day in '08 or '09; (2) collected/bred
            on multiple days in '08 or '09; (3) collected/bred in both '08 and '09; (4) potentially
            collected/bred outside USA.

IV.     Submission of Suspect Positive Samples to the NVSL for Confirmation
        from Approved CEM Laboratories
A swab of suspect colonies should be sent to the NVSL in Amies media with charcoal. If suspect
colonies are noted on multiple anatomical locations from the same set of swabs, send a suspect
colony from each location in individual Amies media. The original swabs do not need to be sent
to the NVSL. Prior to submission of the samples to the NVSL, contact Alan Aalsburg (515-337-
7957 or 515-337-7565) or Dr. Matt Erdman (cell: 515-231-4147). In addition, fax the original
10-4 from the submission to the approved CEM laboratory and the 10-4 submission for
confirmation testing to 515-663-7043. Include both 10-4 forms in with the samples when
shipped.

V.      Reporting of CEM Results from Approved CEM Laboratories
     1. Approved CEM laboratories provide negative results to the submitter (who will be a State
        or Federal animal health official), State Veterinarian, and the AVIC of the submitting
        State by the route that they normally use in that State (email or fax). The submitter is
        responsible for notification of results to the sample collector (accredited veterinarian) and
        the stallion owner.

     2. Approved CEM laboratories promptly report out suspect positive results by email to the
        submitter, State Veterinarian, and AVIC of the submitting State. See above for immediate
        contact requirement with the NVSL for submission for confirmation.

     3. Normal reporting protocols are to be followed for notifications to their State veterinarians
        for samples originating from other States for both negative and suspect positive results.




                                                 2-4
             Laboratory Submission and Reporting Protocol for Active Breeding Stallions
                           Approved CEM Laboratories and the NVSL
                                          January 2010

VI.   Reporting of CEM Culture Results from the NVSL
  1. Negative and positive results are emailed to:
         •    State Veterinarian of submitting State (and designees)
         •    AVIC of submitting State (and designees)
         •    Submitter (State or Federal animal health official)
         •    Approved CEM laboratory that submitted suspect positive
         •    VS EM&D PIC
         •    VS ERMT (if Eastern)
         •    CEM POC for ER (if Eastern)
         •    VS WRMT (if Western)
         •    CEM POC for WR (if Western)
         •    Thomas Bunn
         •    Paul Hauer
         •    Beth Lautner

  2. The submitter is responsible for notification of results to the sample collector (accredited
     veterinarian) and the stallion owner.




                                                2-5
                                                                Appendix 1 – Approved CEM Laboratories
                                                                              January 2010

Laboratory                                Shipping Address                                            Contact 1                              Contact 2

USDA                                      National Veterinary Service Laboratories                    Alan Aalsburg                          Dr. Matt Erdman
                                          1920 Dayton Avenue                                          Office Phone 515-337-7957              Office Phone 515-337-7565
National Veterinary Services              Ames, IA 50010                                              Alt Phone 515-337-7565                 Cell Phone 515-231-4147
Laboratories                                                                                          Fax 515-663-7043                       Email matthew.m.erdman@aphis.usda.gov
                                          Diagnostic Bacteriology Laboratory: 515-663-7568            Email alan.m.aalsburg@aphis.usda.gov
CALIFORNIA                                William R. Pritchard Veterinary Medical Teaching Hospital   Spencer Jang                           JoAnn Yee
                                          Microbiology Lab, Room 1033                                 Phone 530-752-9445                     Phone 530-752-9445
William R. Pritchard Veterinary Medical   One Garrod Drive                                            Fax 530-754-9007                       Fax 530-754-9007
Teaching Hospital, Microbiology Lab       Davis, CA 95616                                             Email ssjang@ucdavis.edu               Email jjyee@vmth.ucdavis.edu

                                          530-752-9445
COLORADO                                  Colorado State University                                   Dr. Barb Powers/Director               Doreen Hyatt, Section Head
                                          Veterinary Diagnostic Laboratory                            Phone 970-297-1281                     Phone 970-297-1281
Colorado State University Diagnostic      300 West Drake Road                                         Fax 970-297-0320                       Fax 970-297-0320
Laboratory                                Fort Collins, CO 80523-1644                                 Email Barb.powers@colostate.edu        Email Drhyatt@colostate.edu

                                          970-297-1281
FLORIDA                                   Florida Dept of Ag and Consumer Sciences                    Dr. James Maxwell                      Dr. Victoria Ilagan Espelita
                                          Animal Disease Diagnostic Lab                               Laboratory Director                    Bacteriology Section
Kissimmee Animal Diagnostic               2700 N. John Young Parkway                                  Office Phone 321-697-1400              Office Phone 321-697-1400
Laboratory                                Kissimmee, FL 34741-1266                                    Cell Phone 386-590-0897                Direct Phone 321-697-1429
Florida Department of Agriculture and                                                                 Fax 321-697-1467                       State Cell 321-624-9141
Consumer Services                         321-697-1400                                                Email maxwelj@doacs.state.fl.us        Fax 321-697-1467
                                                                                                                                             Email espeliv@doacs.state.fl.us
KENTUCKY                                  Livestock Disease Diagnostic Center                         Dr. Mike Donahue                       Dr. Craig Carter
                                          1490 Bull Lea Road                                          Phone 859-253-0571                     Phone 859-253-0571
Livestock Disease Diagnostic Center       Lexington, KY 40511                                         Fax 859-255-1624                       Fax 859-255-1624
University of Kentucky, Lexington                                                                     Email jdonahue@uky.edu                 Email craig.carter@uky.edu
                                          859-253-0571
MARYLAND                                  Animal Health Diagnostic Laboratory                         Dr. Kathy Nepote                       Dr. Jimmy Huang
                                          Maryland Department of Agriculture                          Office Phone 301-600-1548              Phone 301-600-1548
Animal Health Diagnostic Laboratory       1840 Rosemont                                               Cell Phone 301-741-7396                Fax 301-600-6111
Maryland Department of Agriculture        Frederick, MD 21702                                         Fax 301-600-6111                       Email chiminhuang@hotmail.com
                                                                                                      Email knepote@gradschool.umd.edu
                                          301-600-1548
MASSACHUSETTS                             Veterinary-Resources                                        Jim Staruk                             Ron Loranger
                                          1200 Millbury Street, Suite 7K                              Cell Phone 508-450-2864                Phone 401-248-2979
Veterinary Resources                      Worcester, MA 01607                                         Fax 866-421-5298                       Fax 866-421-5298
                                                                                                      Email jim@masshistology.com            Email ron@veterinary-resources.com
                                          877-286-6004, 800-958-9598
MISSOURI                                  Attention Dr. Bill Fales                                    Dr. Bill Fales                         Dr. Tom Reilly
                                          Veterinary Medical Diagnostic Laboratory                    Phone 573-882-6811                     Phone 573-882-6811
Veterinary Medical Diagnostic             1600 East Rollins Street                                    Fax 573-882-1411                       Fax 573-882-1411
Laboratory                                Columbia, MO 65211                                          Email Falesw@missouri.edu              Email reillyt@missouri.edu
University of Missouri
                                          573-882-6811



                                                                                           A1-1
                                                            Appendix 1 – Approved CEM Laboratories
                                                                          January 2010


Laboratory                             Shipping Address                                        Contact 1                                   Contact 2
NEW JERSEY                             NJ Dept of Ag Div of Animal Health                      Denise DiCarlo                              Beatriz Miguel
                                       369 Warren Street                                       Phone 607-777-0139                          Phone 609-292-3965
New Jersey Dept of Ag                  John Fitch Plaza                                               607-777-8395                                 609-777-8395
                                       Trenton NJ 08625                                        Email denise.dicarlo-emery@ag.state.nj.us   Email beatriz.miguel@ag.state.nj.us

                                       609-984-2293
NEW YORK                               Cornell University Vet. School                          Lisa Bowen-Laue                             Dr. Patrick McDonough
                                       Animal Health Diagnostic Laboratory                     Phone 607-253-3938                          Phone 607-253-3927
Cornell University Animal Health       Upper Tower Road                                        Fax 607-253-3907                            Fax 607-253-3943
Diagnostic Laboratory                  Ithaca, NY 14853                                        Email ljb8@cornell.edu                      Email plm2@cornell.edu

                                       607-253-3900
OHIO                                   Animal Disease Diagnostic Laboratory                    Dr. Jing Cui                                Anne Parkinson
                                       Ohio Department of Agriculture                          Bacteriology Section Head                   Microbiology Coordinator
Animal Disease Diagnostic Laboratory   8995 East Main Street, Building 6                       Office Phone 614-728-6220                   Office Phone 614-728-6220
Ohio Department of Agriculture         Reynoldsburg, OH 43068                                  Cell Phone 614-284-7597                     Cell Phone 614-989-8036
                                                                                               Fax 614-728-6310                            Fax 614-728-6310
                                       614-728-6220                                            Email Jcui@agri.ohio.gov                    Email Parkinson@agri.ohio.gov
OKLAHOMA                               Oklahoma Animal Disease Diagnostic Laboratory           Brenda Love                                 Bill Johnson
                                       College of Veterinary Medicine                          Phone 405-744-6623                          Phone 405-744-6623
Oklahoma Animal Disease Diagnostic     Oklahoma State University                               Fax 405-744-8612                            Fax 405-744-8612
Laboratory                             Farm and Ridge Road                                     Email brenda.love@okstate.edu               Email billj.johnson@okstate.edu
                                       Stillwater, OK 74078

                                       405-744-6623
TEXAS                                  Texas Vet Med Diagnostic Laboratory                     Russell Raleigh                             Dr. Robert Sprowls
                                       6610 Amarillo Blvd West                                 Phone 806-353-7478                          Phone 806-353-7478
TVMDL Amarillo                         Amarillo, TX 79106                                      Fax 806-359-0636                            Fax 806-359-0636
                                                                                               Email r-raleigh@tvdml.tamu.edu              Email r-sprowls@tvdml.tamu.edu
                                       806-353-7478
TEXAS                                  Texas Vet Med Diagnostic Laboratory                     Dr. Amy Swinford, Head Bacteriology         Dr. Gayne Fearneyhough, Associate Director
                                       1 Sippel Road                                           Phone 979-845-9000                          Phone 979-845-3414
TVMDL College Station                  College Station, TX 77843                               Fax 979-845-1794                            Fax 979-845-1794
                                                                                               Email aswinford@tvmdl.tamu.edu              Email gfearneyhough@tvmdl.tamu.edu
                                       979-845-3414
VIRGINIA                               Harrisonburg Regional Animal Health Laboratory          Karen Burris                                Dr. David Brown
                                       261 Mount Clinton Pike                                  Phone 540-209-9130                          Phone 540-209-9130
VDACS Harrisonburg Regional Animal     Harrisonburg, VA 22802                                  Fax 540-432-1195                            Fax 540-432-1195
Health Laboratory                                                                              Email Karen.burris@vdacs.virginia.gov       Email david.brown@vdacs.virginia.gov
                                       540-209-9130
VIRGINIA                               Warrenton Regional Animal Health Laboratory             Susan L. Brown                              Richard Sexton/Micro Supervisor
                                       272 Academy Hill Road                                   Phone 540-347-6385                          Phone 540-347-6385
VDACS Warrenton Regional Animal        Warrenton, VA 20186                                     Fax 540-347-6404                            Fax 540-347-6404
Health Laboratory                                                                              Email Susan.brown@vdacs.virginia.gov        Email Richard.sexton@vdacs.virginia.gov
                                       540-347-6385



                                                                                        A1-2
Appendix 2 - Example of VS Form 10-4 Specimen Submission - Active Breeding Stallion
 $FFRUGLQJ WR WKH 3DSHUZRUN 5HGXFWLRQ $FW RI  DQ DJHQF\ PD\ QRW FRQGXFW RU VSRQVRU DQG D SHUVRQ LV QRW UHTXLUHG WR UHVSRQG WR D FROOHFWLRQ RI LQIRUPDWLRQ XQOHVV LW GLVSOD\V
                                                                                                                                                                                         20% $SSURYHG
 D YDOLG 20% FRQWURO QXPEHU 7KH YDOLG 20% FRQWURO QXPEHU IRU WKLV LQIRUPDWLRQ FROOHFWLRQ LV   DQG  7KH WLPH UHTXLUHG WR FRPSOHWH WKLV LQIRUPDWLRQ FROOHFWLRQ
                                                                                                                                                                                       
 LV HVWLPDWHG WR DYHUDJH  KRXUV SHU UHVSRQVH IRU   KRXU SHU UHVSRQVH IRU  DQG  KRXUV SHU UHVSRQVH IRU  LQFOXGLQJ WKH WLPH IRU UHYLHZLQJ
                                                                                                                                                                                         DQG 
 LQVWUXFWLRQV VHDUFKLQJ H[LVWLQJ GDWD VRXUFHV JDWKHULQJ DQG PDLQWDLQLQJ WKH GDWD QHHGHG DQG FRPSOHWLQJ DQG UHYLHZLQJ WKH FROOHFWLRQ RI LQIRUPDWLRQ

           81,7(' 67$7(6 '(3$570(17 2) $*5,&8/785(                                                                                                                                     3$*(
          $1,0$/ $1' 3/$17 +($/7+ ,163(&7,21 6(59,&(
         1$7,21$/ 9(7(5,1$5< 6(59,&(6 /$%25$725,(6                                                              63(&,0(1 68%0,66,21                                                           2)
        32 %2;   '$<721 $9(18( $0(6 ,$ 
                             
 ,16758&7,216 8VH D VHSDUDWH IRUP IRU HDFK VSHFLHV DQG HDFK RZQHUEURNHU 6HH ³,QVWUXFWLRQV IRU &RPSOHWLQJ 96 )RUP ´ IRU GHILQLWLRQV
  68%0,77(5 1$0( including Business Name                                 196/ 68%0,77(5 ,'               1$0( 2) 2:1(5                                                 &KHFN LI ZLOGOLIH no owner

  State or Federal animal health official                                                                                                  Stallion owner's name
 (0$,/ $''5(66                                                                                                                            2:1(5 &,7<                                   67$7(&28175<


 3+21( 12                                                 )$; 12                                                                         /2&$7,21 2) $1,0$/6
 0$,/,1* $''5(66 Street, City, State, ZIP Code                                                                                          35(0,6(6 ,'

                                                                                                                                          &2817<                                       67$7(&28175<

                                                                                                                                           Stallion's location
  3$<0(17 0(7+2'
      86(5 )(( $&&2817 12                                                &+(&.021(< 25'(5                                                             1XPEHU
                                                                        Enclosed, payable to USDA in US dollars                             &5(',7
LEAVE PAYMENT BLANK                                                                                                                           &$5'      ([S 'DWH




                                                                                                                                                   E
      +(5')/2&. 6,=(                  (;$0,1$7,216 5(48(67('                                                                                    &2//(&7(' %<

                                       CEM Culture (time collected: 9 a.m. EST)                                                                          Accredited Vet who collected




                                                                                                                                                 L
      12 ,1 +(5')/2&.                                                                                                                               '$7( &2//(&7('
     $))(&7('                                                                                                                                             mm/dd/2010
      12 ,1 +(5')/2&.                                                                                                                               $87+25,=(' %<




                                                                                                   P
     '($'                                                                                                                                                AVIC of submitting State
      385326( 2) 68%0,66,21 See instructions for definitions                                                                                       &28175< 2) 25,*,1'(67,1$7,21
       ,QWHUVWDWH 0RYHPHQW                       ,PSRUW                             7%                                    5HDJHQW (YDOXDWLRQ




                                                                                                 M
                                                                                                                                                        5()(55$/ 180%(5
       ([SRUW                                    )$'(3 'LDJQRVWLF                   *HQHUDO 'LDJQRVWLF                   196/ ,QWUDODE
       3UH,PSRUW                                6XUYHLOODQFH                        'HYHORSPHQWDO 5HVHDUFK                                            CEM-SURV - reference number




                                                   A
   35(6(59$7,21
     1RQH   ,FH 3DFN               'U\ ,FH         )RUPDOLQ        %RUD[         $OFRKRO         2WKHU Specify
      63(&,0(16 68%0,77(' “X” applicable item(s)                                                                                                                    727$/ 180%(5 2)




                                                 X
                                                                                                                                                                        63(&,0(16 68%0,77('
       %ORRG           )HFHV            3DUDVLWH            6HUXP                        7LVVXH Vpecify             :KROH $QLPDO               2WKHU specify
       &XOWXUH         )HHG             3ODQW               6RLO                         8ULQH                        )HWXV
                                                                                                                                                                        3




                           E
       ([WUDFW         0LON             6HPHQ               6ZDE specify               :DWHU                        '1$51$

      63(&,(6 25 6285&( “X” ONLY one                                                                                                                                180%(5 2) $1,0$/6
                                                                                                                                                                        6$03/('
       &DWWOH                    *RDW                &KLFNHQ                             %LVRQ                     )LVK                       2WKHU specify
       6ZLQH                     +RUVH               7XUNH\                              'HHU specify            (QYLURQPHQW
                                                                                                                                                                       1
       6KHHS                     'RQNH\              2WKHU ELUG specify                (ON                       5HDJHQW

                  ,'(17,),&$7,21 See instructions  VDPSOHV SHU IRUP                                                                          ,'(17,),&$7,21
     6DPSOH ,'                    $QLPDO ,'                    %UHHG         $JH                            6H[      6DPSOH ,'                   $QLPDO ,'           %UHHG                    $JH      6H[


A- dis urethra      "George" / tattoo 9876A                              QH                      12         M       XXXXXXX XXXXXXXXXXXXXXXXX                               XXXXXXX XXX XXX
B- ureth sinus       "George" / tattoo 9876A                             QH                      12         M       XXXXXXX XXXXXXXXXXXXXXXXX                              XXXXXXX XXX XXX
C- fos glandis       "George" / tattoo 9876A                             QH                      12         M       XXXXXXX XXXXXXXXXXXXXXXXX                               XXXXXXX XXX XXX
XXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXX                                                  XXX XXX            XXXXXXX XXXXXXXXXXXXXXXXX                              XXXXXXX XXX XXX
XXXXXXXXXX XXXXXXXXXXXXXXXXXXXXXXXXX XXXXXXXXXX                                                  XXX XXX            XXXXXXX XXXXXXXXXXXXXXXXX                              XXXXXXX XXX XXX
  $'',7,21$/ '$7$ History, clinical signs, post mortem findings, remarks, tentative diagnosis, special instructions. Use additional sheets if necessary
- Registered/Full stallion name: "Gorgeous George"             ** CEM surveillance stallion **
- Registration number: 3456789
- Indicate priority category(ies) of stallion: [1] collected/bred with other stallion(s) on same day in '08 or '09; [2] collected/bred on multiple days in '08 or '09;
                                               [3] collected/bred in both '08 & '09; [4] potentially collected/bred outside USA.
  6,*1$785( 2) 68%0,77(5 $1' '$7(                                                                                                                                196/ 86( 21/<

 ;
                                                           196/ 86( 21/<
 &21',7,21                    35,25,7<                    ',675,%87,21                     5(&(,9(' %<

 96 )250 
 $8* 
                                                                                                 A2-1
Appendix 2 - Example of VS Form 10-4 Specimen Submission - Active Breeding Stallion
 96 )250  ,16758&7,216
                                                                                               ,QWHUVWDWH 0RYHPHQW ± 7HVWV FRQGXFWHG IRU WKH SXUSRVH RI TXDOLI\LQJ OLYH DQLPDOV
 $// LQIRUPDWLRQ PXVW EH SULQWHG OHJLEO\ RU W\SHG 8VH D VHSDUDWH IRUP IRU HDFK                RU SRXOWU\ IRU LQWHUVWDWH PRYHPHQW
 VSHFLHV DQG RZQHU $W WKH PLQLPXP FRPSOHWH DOO ILHOGV GHVLJQDWHG LQ WKHVH                    ([SRUW ± 7HVWV FRQGXFWHG IRU WKH SXUSRVH RI TXDOLI\LQJ DQLPDOV RU SRXOWU\
 LQVWUXFWLRQV DV UHTXLUHG &RQWDFW WKH 5HFHLYLQJ 'HSDUWPHQW RI WKH ODERUDWRU\ WR               LQFOXGLQJ ZLOG DQLPDOV DQG ELUGV RU DQLPDO RU SRXOWU\ SURGXFWV IRU H[SRUW IURP WKH
 ZKLFK \RX DUH VHQGLQJ VSHFLPHQV ZLWK VSHFLILF GRFXPHQWDWLRQ RU VKLSSLQJ                       86 WR D IRUHLJQ FRXQWU\
 TXHVWLRQV                                                                                    3UH,PSRUW ± 7HVWV FRQGXFWHG IRU WKH SXUSRVH RI TXDOLI\LQJ DQLPDOV RU SRXOWU\
                                                                                               LQFOXGLQJ ZLOG DQLPDOV DQG ELUGV RU DQLPDO RU SRXOWU\ SURGXFWV IRU LPSRUW LQWR WKH
 ,I LQFOXGLQJ PRUH WKDQ RQH SDJH LQFOXGH WKH SDJH QXPEHU RI WRWDO SDJHV                       86 6HOHFW WKLV SXUSRVH ZKHQ WKH DQLPDOV RU SURGXFWV KDYH QRW \HW EHHQ PRYHG
 VXEPLWWHG e.g., 1 of 3                                                                     LQWR WKH 86
                                                                                               ,PSRUW ± 7HVWV FRQGXFWHG IRU WKH VDPH SXUSRVH DV SUHLPSRUW H[FHSW WKDW WKH
  68%0,77(5 &217$&7 ,1)250$7,21                                      ³5(48,5('´              DQLPDOV RU SURGXFWV DUH FXUUHQWO\ ORFDWHG DW D 86 LPSRUW FHQWHU
 (QWHU WKH VXEPLWWHU¶V EXVLQHVV QDPHDIILOLDWLRQ WKH QDPH RI WKH LQGLYLGXDO                   )$'(3 'LDJQRVWLF ± 7HVWV FRQGXFWHG IRU WKH SXUSRVH RI GLDJQRVLQJ RU
 VXEPLWWHU LV RSWLRQDO LI WHVW UHVXOWV DUH UHWXUQHG WR D JHQHUDO EXVLQHVV ID[ HPDLO          FRQILUPLQJ D IRUHLJQ GLVHDVH RU IRU WKH HUDGLFDWLRQ RI D IRUHLJQ GLVHDVH WKDW KDV
 RU PDLOLQJ DGGUHVV (QWHU D ID[ QXPEHU RU HPDLO DGGUHVV WR ZKLFK ZH FDQ UHWXUQ                JDLQHG HQWUDQFH LQWR WKH 86 ,I D IRUHLJQ DQLPDO GLVHDVH LV VXVSHFWHG IROORZ
 WHVW UHVXOWV 0XOWLSOH HPDLO DGGUHVVHV DUH SHUPLVVLEOH 6SHFLI\ LI WKHUH LV D                 LQVWUXFWLRQV LQ %ORFN  IRU DXWKRUL]DWLRQ WR VXEPLW D )$' VSHFLPHQ
 SUHIHUUHG PHWKRG RI UHSRUW GHOLYHU\ HPDLO ZLOO EH XVHG LI QR SUHIHUHQFH LV VWDWHG           6XUYHLOODQFH ± 7HVWV FRQGXFWHG IRU PRQLWRULQJ IRU D VSHFLILF GLVHDVH IRU D
 3URYLGH D FRPSOHWH PDLOLQJ DGGUHVV ,I ID[ RU HPDLO LV QRW DYDLODEOH WHVW UHSRUWV            VSHFLILF LQVHFW RU LQVHFW YHFWRU RU IRU DQDO\]LQJ VSHFLILF SURGXFWV WKDW DUH XVHG LQ
 FDQ EH PDLOHG EXW WKLV ZLOO GHOD\ GHOLYHU\ RI \RXU UHVXOWV 5HSHDW VXEPLWWHUV DUH            WUHDWLQJ DQLPDOV RU SRXOWU\ RU IRU GHFRQWDPLQDWLRQ RI DQLPDO SRXOWU\ IDFLOLWLHV
 HQFRXUDJHG WR EH FRQVLVWHQW ZLWK WKH VXEPLWWHU FRQWDFW LQIRUPDWLRQ WKDW WKH\                  7% ± 7HVWV FRQGXFWHG IRU GLDJQRVLQJ 7XEHUFXORVLV
 SURYLGH DV WKH 196/ NHHSV D PDVWHU UHFRUG ,I WKH WHVW UHSRUW IRU DQ LQGLYLGXDO              *HQHUDO 'LDJQRVWLF &DVH ± 7HVWV FRQGXFWHG IRU WKH SXUSRVH RI GLDJQRVLQJ RU
 VXEPLVVLRQ QHHGV WR EH URXWHG WR D QRQVWDQGDUG GHVWLQDWLRQ LQFOXGH VSHFLDO                  FRQILUPLQJ D GRPHVWLF GLVHDVH DQGRU WKH DQDO\VLV RI HQYLURQPHQWDO SURGXFWV
 LQVWUXFWLRQV LQ %ORFN  $GGLWLRQDO 'DWD                                                    WKDW PD\ EH FRQWULEXWLQJ WR DQ H[LVWLQJ GLVHDVH FRQGLWLRQ 8VH WKLV SXUSRVH ZKHQ
                                                                                               WKH SXUSRVHV OLVWHG DERYH GR QRW DSSO\
  196/ 68%0,77(5 ,'                                                                          'HYHORSPHQWDO5HVHDUFK ± 7HVWV FRQGXFWHG IRU WKH SXUSRVH RI VXSSRUWLQJ D
 )RU PRUH HIILFLHQW VHUYLFH UHSHDW VXEPLWWHUV DUH HQFRXUDJHG WR LQFOXGH WKHLU                 GHYHORSPHQWDO RU UHVHDUFK SURMHFW FRQGXFWHG E\ VWDII RU ILHOG SHUVRQQHO RI 96 RU
 196/ 6XEPLWWHU ,' ,I \RX GR QRW NQRZ \RXU ,' FRQWDFW WKH 196/ DW                            E\ RWKHU ODERUDWRULHV LQVWLWXWLRQV RU DJHQFLHV
                                                                                 5HDJHQW (YDOXDWLRQ ± 7HVWV FRQGXFWHG IRU WKH SXUSRVH RI HYDOXDWLQJ D UHDJHQW
                                                                                               SURGXFHG E\ RWKHU ODERUDWRULHV LQVWLWXWLRQV RU DJHQFLHV
  2:1(5 ,1)250$7,21                                            ³5(48,5('´                    196/ ,QWUDODE ± 7HVWV FRQGXFWHG IRU DQRWKHU ODERUDWRU\ RI WKH 196/
 (QWHU WKH FRPSOHWH QDPH RI WKH DQLPDO RZQHU WKH FLW\ DQG WKH WZROHWWHU




                                                                                                                                 E
 DEEUHYLDWLRQ RI WKH 6WDWH LQ ZKLFK WKH RZQHU UHVLGHV (QVXUH WKH DQLPDO RZQHU LV               &28175< 2) 25,*,1'(67,1$7,21
 LGHQWLILHG KHUH DQG QRW WKH SURSHUW\ PDQDJHU RU YHWHULQDULDQ )RU ZLOGOLIH FKHFN             )RU LPSRUW RU SUHLPSRUW FDVHV HQWHU WKH FRXQWU\ LQ ZKLFK WKH DQLPDOV ODVW




                                                                                                                               L
 WKH ER[ WR LQGLFDWH WKHUH LV QR RZQHU                                                        UHVLGHG )RU H[SRUW FDVHV HQWHU WKH FRXQWU\ WR ZKLFK WKH DQLPDOV ZLOO EH
                                                                                               VKLSSHG
  /2&$7,21 2) 7+( $1,0$/6                                       ³5(48,5('´
 ,QFOXGH 1DWLRQDO $QLPDO ,GHQWLILFDWLRQ 6\VWHP 1$,6 SUHPLVHV ,' LI DYDLODEOH                 5()(55$/ 180%(5




                                                                                      P
 $OVR VSHFLI\ WKH FRXQW\ SDULVK RU RWKHU GHVLJQDWHG ORFDWLRQ RI WKH DQLPDOV DQG              7KLV QXPEHU LV W\SLFDOO\ DVVLJQHG E\ WKH VXEPLWWHU DQG LV XVHG IRU WKH VXEPLWWHU¶V
 WKH WZROHWWHU 6WDWH DEEUHYLDWLRQ                                                            RZQ UHIHUHQFH ,Q )$' FDVHV HQWHU WKH LQYHVWLJDWLRQ FRQWURO QXPEHU GHVFULEHG
                                                                                               LQ WKH LQVWUXFWLRQV IRU %ORFN 
  3$<0(17 0(7+2'                           ³5(48,5(' )25 %,//$%/( &$6(6´




                                                                                    M
 &KHFN WKH DSSURSULDWH SD\PHQW PHWKRG ,I SD\PHQW LV E\ XVHU DFFRXQW RU FUHGLW                  35(6(59$7,21
 FDUG HQWHU WKH DFFRXQW QXPEHU (QWHU WKH H[SLUDWLRQ PRQWK DQG \HDU ZKHQ XVLQJ                &KHFN DOO EORFNV WKDW DSSO\
 D FUHGLW FDUG 5HIHU WR WKH 8VHU )HHV3D\PHQW 2SWLRQV DQG WKH &DWDORJ RI
 6HUYLFHV)HHV ERWK ORFDWHG DW                                                                 63(&,0(16 68%0,77('                                              ³5(48,5('´




                                              A
 ZZZDSKLVXVGDJRYDQLPDOBKHDOWKODEBLQIRBVHUYLFHVGLDJQRVBWHVWVVKWPO IRU                   &KHFN DOO EORFNV WKDW DSSO\
 VSHFLILF WHVW IHHV DQG D OLVW RI DFFHSWHG FUHGLW FDUGV '2 127 6(1' &$6+
                                                                                                727$/ 180%(5 2) 63(&,0(16 68%0,77('




                                            X
  +(5')/2&. 6,=(                                                                            (QWHU WKH WRWDO QXPEHU RI VSHFLPHQV VXEPLWWHG 6SHFLPHQV LQ RQH FRQWDLQHU DUH
 (QWHU WKH WRWDO QXPEHU RI DQLPDOV LQ WKH KHUGIORFN                                          FRXQWHG DV RQH VDPSOH 3OHDVH OLPLW WR  VDPSOHV SHU VXEPLVVLRQ

  12 ,1 +(5')/2&. $))(&7('                                                                  63(&,(6 25 6285&(                                             ³5(48,5('´




                          E
 (QWHU WKH WRWDO QXPEHU RI DQLPDOV LQ GLUHFW FRQWDFW ZLWK VXVSHFW DQLPDO RU                    &KHFN RQO\ RQH EORFN ,I VSHFLPHQV DUH IURP GLIIHUHQW VSHFLHV RU VRXUFHV XVH D
 VKRZLQJ FOLQLFDO VLJQV                                                                       VHSDUDWH 96 )RUP  IRU HDFK VRXUFH 5HPLQGHU (QWHU WKH DQLPDO %5((' LQ
                                                                                               %ORFN 
  12 ,1 +(5')/2&. '($'
 (QWHU WKH WRWDO QXPEHU RI DQLPDOV IURP WKLV KHUGIORFN WKDW DUH GHDG                          180%(5 2) $1,0$/6 6$03/('
                                                                                               (QWHU WKH WRWDO QXPEHU RI DQLPDOV VDPSOHG
  (;$0,1$7,216 5(48(67('                                          ³5(48,5('´
 )RU GLVHDVH SURJUDPV LW LV QHFHVVDU\ RQO\ WR HQWHU WKH SURJUDP QDPH e.g.,                    ,'(17,),&$7,21                                                 ³5(48,5('´
 CWD, Scrapie, or BSE ,I WKH WHVW LV QRW IRU D GLVHDVH SURJUDP VSHFLI\ WKH                  6DPSOH ,' ± ,GHQWLI\ VDPSOHV ZLWK FRQVHFXWLYH QXPEHUV (QVXUH WKH VDPSOH
 GLVHDVH DQG WKH GHVLUHG WHVW                                                                 LGHQWLILFDWLRQ QXPEHU RQ WKLV IRUP PDWFKHV WKH VDPSOH LGHQWLILFDWLRQ
                                                                                               QXPEHU SODFHG RQ WKH VSHFLPHQ FRQWDLQHU
  &2//(&7(' %<                                                                              $QLPDO ,' ± 5HFRUG WKH DQLPDO¶V QDWLRQDO LGHQWLILFDWLRQ WDJ QXPEHU DGMDFHQW WR
 (QWHU WKH FRPSOHWH QDPH RI WKH SHUVRQ FROOHFWLQJ WKH VSHFLPHQs                             WKH DSSURSULDWH VDPSOH QXPEHU ,I WKHUH LV QR QDWLRQDO DQLPDO ,' UHFRUG WKH
                                                                                               PRVW DSSURSULDWH LGHQWLILFDWLRQ QXPEHU or name 127( /DERUDWRU\ UHVXOWV ZLOO
  '$7( &2//(&7('                                                                            EH UHSRUWHG E\ DQLPDO LGHQWLILFDWLRQ QXPEHU
 (QWHU WKH GDWH RQ ZKLFK VSHFLPHQV ZHUH FROOHFWHG 8VH WKH IRUPDW                              %UHHG ± (QWHU WKH DQLPDO EUHHG e.g., Holstein, Angus
 ''00<<<<                                                                                   $JH ± ,QGLFDWH WKH DSSUR[LPDWH DJH LQ \HDUV y PRQWKV m ZHHNV w RU GD\V
                                                                                               d
  $87+25,=(' %<                                                                             6H[ ± ,QGLFDWH WKH VH[ PDOH M RU IHPDOH F IRU HDFK DQLPDO
 (QWHU WKH QDPH RI WKH SHUVRQ DXWKRUL]LQJ WKH VXEPLVVLRQ RI WKLV VDPSOH
 1RUPDOO\ WKLV LV WKH $UHD 9HWHULQDULDQ LQ &KDUJH AVIC LQ \RXU 6WDWH                        $'',7,21$/ '$7$
 $XWKRUL]DWLRQ LV DVVXPHG IRU UHJXODWRU\ YHWHULQDULDQV PDNLQJ URXWLQH SURJUDP                  (QWHU DOO SHUWLQHQW LQIRUPDWLRQ DERXW WKH DQLPDOV DQG SUHPLVHV WKDW FDQ DVVLVW WKH
 VSHFLPHQ VXEPLVVLRQV 6HH                                                                     ODE LQ PDNLQJ D GLDJQRVLV
 KWWSZZZDSKLVXVGDJRYDQLPDOBKHDOWKDUHDBRIILFHV WR ORFDWH WKH $9,& LQ \RXU              x      3URYLGH GHWDLO RQ WLVVXH VSHFLPHQV \RX DUH LQFOXGLQJ e.g., lymph nodes,
 ORFDO DUHD                                                                                          obex, brain
 ,I DQ H[RWLF foreign GLVHDVH LV VXVSHFWHG FRQWDFW WKH $9,& DQG WKH (PHUJHQF\               x      6SHFLI\ FOLQLFDO VLJQV e.g., weight loss, hair missing
 3URJUDPV VWDII WR REWDLQ DXWKRUL]DWLRQ WR VXEPLW VDPSOHV IRU )$' WHVWLQJ DQG DQ               x      ,I PHDW LV EHLQJ UHWDLQHG SHQGLQJ VSHFLPHQ UHVXOWV HQWHU 5(7$,1('
 LQYHVWLJDWLRQ FRQWURO QXPEHU WKDW PXVW EH LQFOXGHG ZLWK WKH VXEPLVVLRQ '2                    x      $GG UHODWHG FDVH VXEPLVVLRQ QXPEHUV WR DVVLVW LQ WUDFH DFWLYLWLHV
 127 VKLS DQ\ VXFK VSHFLPHQV XQWLO DSSURYDO LV UHFHLYHG DQG D FRQWURO QXPEHU LV                x      ,QFOXGH DQ\ LQIRUPDWLRQ WKDW GLG QRW ILW LQWR LWV GHVLJQDWHG VSDFH
 DVVLJQHG 7KH UHFHLSW RI DQ XQDXWKRUL]HG VKLSPHQW RI VSHFLPHQV FRQWDLQLQJ                            HOVHZKHUH RQ WKH IRUP
 H[RWLF GLVHDVH DJHQWV FDQ FDXVH VXEVWDQWLDO GLVUXSWLRQ RI ZRUN DW WKH ODERUDWRU\              x      ,QFOXGH DQ\ VSHFLDO non-standard LQVWUXFWLRQV IRU WHVW UHSRUW GHOLYHU\
 DQG UHVXOW LQ SRVVLEOH ILQHV IRU WKH VXEPLWWHU
                                                                                                6,*1$785( 2) 68%0,77(5 $1' '$7(
  385326( 2) 68%0,66,21                                            ³5(48,5('´               7KH LQGLYLGXDO VXEPLWWLQJ WKH VSHFLPHQs PXVW VLJQ DQG GDWH WKH IRUP
 'HILQLWLRQV RI 'LDJQRVWLF &DVH &DWHJRULHV DUH DV IROORZV
 96 )RUP  Reverse)


                                                                                        A2-2
Stallion Reproductive Anatomical Photographs

 Stallion – Urethral Sinus




                     3-1
Stallion Reproductive Anatomical Photographs

 Stallion – Urethral Sinus




                     3-2
Stallion Reproductive Anatomical Photographs

   Stallion – Fossa Glandis




                     3-3
Stallion Reproductive Anatomical Photographs

   Stallion - Distal Urethra




                     3-4
Stallion Reproductive Anatomical Photographs

   Stallion - Distal Urethra




                     3-5