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The Saccharomyces cerevisiae Actin Cytoskeletal Component Bsp1p Has an Auxiliary Role in Actomyosin Ring Function and in the Maintenance of Bud-Neck Structure by ProQuest

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Iqg1p is a component of the actomyosin contractile ring that is required for actin recruitment and septum deposition. Cells lacking Iqg1p function have an altered bud-neck structure and fail to form a functional actomyosin contractile ring resulting in a block to cytokinesis and septation. Here it is demonstrated that increased expression of the actin cytoskeleton associated protein Bsp1p bypasses the requirement for contractile ring function. This also correlates with reduced bud-neck width and remedial septum formation. Increased expression of this protein in a temperature-sensitive iqg1-1 background causes remedial septum formation at the bud neck that is reliant upon chitin synthase III activity and restores cell separation. The observed suppression correlates with a restoration of normal bud-neck structure. While Bsp1p is a component of the contractile ring, its recruitment to the bud neck is not required for the observed suppression. Loss of Bsp1p causes a brief delay in the redistribution of the actin cytoskeleton normally observed at the end of actin ring contraction. Compromise of Iqg1p function, in the absence of Bsp1p function, leads to a profound change in the distribution of actin and the pattern of cell growth accompanied by a failure to complete cytokinesis and cell separation. [PUBLICATION ABSTRACT]

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									Copyright Ó 2008 by the Genetics Society of America
DOI: 10.1534/genetics.107.082685



       The Saccharomyces cerevisiae Actin Cytoskeletal Component Bsp1p Has
            an Auxiliary Role in Actomyosin Ring Function and in the
                       Maintenance of Bud-Neck Structure

   Daniel J. Wright,*,1 Ewen Munro,*,1 Mark Corbett,*,1 Adam J. Bentley,* Nigel J. Fullwood,*
                              Stephen Murray† and Clive Price*,2
      *Biomedical Sciences Unit, Biological Sciences, Lancaster University, Lancaster LA1 4YQ, United Kingdom and †Patterson Institute
                      for Cancer Research, University of Manchester, Withington, Manchester M20 4BX, United Kingdom
                                                         Manuscript rece
								
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