Reduced Mismatch Repair of Heteroduplexes Reveals "Non"-interfering Crossing Over in Wild-Type Saccharomyces cerevisiae by ProQuest

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									Copyright Ó 2008 by the Genetics Society of America
DOI: 10.1534/genetics.106.067603



   Reduced Mismatch Repair of Heteroduplexes Reveals ‘‘Non’’-interfering
            Crossing Over in Wild-Type Saccharomyces cerevisiae

       Tony J. Getz,1 Stephen A. Banse,2 Lisa S. Young, Allison V. Banse,2 Johanna Swanson,3
          Grace M. Wang,4 Barclay L. Browne, Henriette M. Foss and Franklin W. Stahl5
                 Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, Oregon 97403-1229
                                                        Manuscript received October 31, 2006
                                                      Accepted for publication January 26, 2008


                                                               ABSTRACT
               Using small palindromes to monitor meiotic double-strand-break-repair (DSBr) events, we demonstrate
             that two distinct classes of crossovers occur during meiosis in wild-type yeast. We found that crossovers
             accompanying 5:3 segregation of a palindrome show no conventional (i.e., positive) interference, while cross-
             overs with 6:2 or normal 4:4 segregation for the same palindrome, in the same cross, do manifest interference.
             Our observations support the concept of a ‘‘non’’-interference class and an inter
								
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