NAME Taq DNA Polymerase by alj19178

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									                                                                                                                           NAME: Taq DNA Polymerase




 Taq DNA Polymerase                                                                                                                                                          lele    Biotechnology
                                                                                      9924 Mesa Rim Rd. ~San Diego, CA 92121
                                                                                           (O) 858.587.6645 ~ (F) 858.587.6692
                                                                                                         www.allelebiotech.com


Catalog Numbers:

Taq DNA Polymerase, 5 units/µL
ABP-PP-ERTQ100          100 Units                                         Storage Condition:                                          -20°C
ABP-PP-ERTQ100          200 Units
ABP-PP-ERTQ500          500 Units
ABP-PP-ERTQ001         1000 Units
                                                                          Unit Definition:
Description:
                                                                          One unit is defined as the amount of enzyme necessary to
Taq DNA polymerase is a thermostable DNA polymerase that                  catalyze the incorporation of 0.01 µmol dNTP into acid-insolu-
possesses a 5’ > 3’ polymerase activity and a double strand               ble material in 30 min at 72°C in 1X reaction buffer.
specific 5’ > 3’ exonuclease activity. It is the most commonly
used enzyme for PCR.                                                      Storage Conditions:

Source:                                                                   100mM KCl, 10 mM Tris-HCl (ph 7.4), 0.1 mM EDTA. 1 mM
                                                                          dithiothreitol, 0.5% Tween 20, 0.5% NP-40 and 50% glycerol
Purified from an E. coli strain that carries the recombinant Taq
DNA Polymerase gene.                                                      Typical Use:
                                                                          Add 1-5 units of Taq Polymerase per 50uL PCR reaction (i.e.
Applications:                                                             0.2-1uL of enzyme).
- PCR Amplification
- Primer extension
                                                                             Activity Comparison of Taq DNA Polymerases
Reaction Buffer:                                                                       from Different Sources

1X Reaction Buffer [20mM Tris-HCL (pH 8.8 @ 25 °C), 10 mM
(NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1% Triton x-100].

Quality Control:

Each lot of the enzyme has been tested for purity and activity.
Free of contaminants such as
                                                                                                                                      Competitor E




                                                                                                                                                                      Competitor E
                                                                                                                                                     Allele Enzymes
                                                                                       Allele Enzymes



                                                                                                                       Competitor P
                                                                                                        Competitor I




                                                                                                                                                                                     Competitor P

                                                                                                                                                                                                    Competitor E

endonucleases or exonucleases.



NOTES:

______________________________________________
______________________________________________
_______________________________________________________________________       The Taq DNA polymerase produced by Allele
_______________________________________________________________________       Enzymes, a division of Allele Biotech, consistently
___________________________________________________                           outperforms Taq DNA polymerase from well known
___________________________________________________                           suppliers.
___________________________________________________
___________________________________________________                           Polymerase Chain Reactions were performed with polymer-
___________________________________________________                           ase from different companies under the same conditions.
___________________________________________________                           After only 16 cycles of reaction, 3 ml of each reaction was
___________________________________________________                           run on 1% agarose gel.
___________________________________________________
___________________________________________________
___________________________________________________

								
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