; Micropropagation of Alocasia amazonica using semisolid and liquid cultures
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Micropropagation of Alocasia amazonica using semisolid and liquid cultures

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An efficient, simple micropropagation method was developed for Alocasia amazonica using corms in semisolid and liquid cultures. Explants were cultured onto Murashige and Skoog (MS) medium (Murashige and Skoog, Physiol. Plant. 15:473-497, 1962) supplemented with different cytokinins (Benzyladenine [BA, 2.22-13.32 M], kinetin [2.32-13.95 M], Thidiazuron [TDZ, 0.45-4.54 M]) and cytokinin in combination with auxins [naphthalene acetic acid (NAA, 0.54-5.37 M)/indole acetic acid (IAA, 0.57-5.71 M)//indole butyric acid (IBA, 0.49-4.9 M)]. All supplementary-induced shoot proliferation and the optimal results was on the medium supplemented with 2.27 M TDZ, which induced 5.1 shoots per explant. Among the different concentrations of sucrose (0-120 g 1^sup -1^) tested for shoot proliferation, 30 g 1^sup -1^ was found suitable for corm cultures of Alocasia amazonica. The optimal shoot proliferation and biomass values were with the plantlets grown at 30 mol m^sup -2^ s^sup -1^ photosynthetic photon flux (PPF) and 25C. Liquid cultures found suitable for shoot proliferation and biomass accumulation was compared to semisolid cultures. Comparative studies of bioreactor systems [continuous immersion (with or without net) and temporary immersion in liquid media using ebb and flood] revealed that shoot multiplication and growth were greatest with the raft bioreactor system. Plantlets (cormlets) from the bioreactor were hydroponically cultured for 30 days, and 100% of plants were acclimatized successfully. The simple efficient method of production of plantlets (cormlets) is useful for large-scale multiplication of this important ornamental plant. [PUBLICATION ABSTRACT]

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