ATPmetry a tool in water quality management by mpm74462

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									ATPmetry: a tool in water
  quality management
        Michel Hignette
   Aquarium de la Porte Dorée
             Paris
                   ATPmetry
 A rapid method to method to measure
  levels of microorganisms in water samples
 Adenosine Tri-Phosphate (ATP)
     Is present in all animal and vegetable matter
      (including microorganisms)
     Can be measured by using an enzyme reaction
      (naturally occurring in the tails of fireflies)
    Luciferin + Luciferase + ATP -> light


                 1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
             Light production


                ATP        AMP + PPi

Luciferin

                 Catalysis with Mg2+                       Light
Luciferase


                O2              CO2




               1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
         Light measurement
 Light emitted is in proportion to the amount
  of ATP
 The intensity of light is given in Relative
  Light Units (RLU)
 Total ATP in water can be used to monitor
  the efficiency of biocide treatment
  programs (UV, ozone, oxidising agents...)



              1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
         Measurement method
   Remove the sampling stick from the
    device and immerse the sampling rings
    into the liquid under test




               1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
        Measurement method (2)
   Push down on the top of the handle
    Releases the firefly reagent
    => Reaction with the ATP collected on the
     sampling rings




                1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
       Measurement method (3)
   Insert the device in the lightmeter




                1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
    Measurement method (4)
 The light emitted by the sample is
  measured and the result in RLU appears
  on the display
 The level of ATP is proportional to the
  amount of light produced by the test




             1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
     Measurement comparisons
   2 brands of ATPmeters were tested




               1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
                       Results
                Uni-Lite Xcel®                        Hy-Lite 2®
                  (en RLU)                             (en URL)
 Carangues   426-439-681-435-411 1200-22-1000-810-43


  Discus     207-210-197-149-198               98-106-12-140-131


Hippocampus 312-325-501-317-335                 150-110-8-130-86


 Piranhas    674-280-304-306-298               127-110-97-199-90




                 1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
          Filtration monitoring
 Decantation tank: 120 RLU
 Before filtration: 31 RLU
 After filtration: 17 RLU
 After UV sterilisation: 6 RLU
 Recontamination in the distribution pipes:
     Plug 1: 43 RLU
     Plug 2: 56 RLU
     Plug 3: 62 RLU


                1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
                Water disposal
   European Union Zoo Directives:
       No animal escape from zoos
   French legislation:
     no escape (including microorganisms)
     ATPmetry may be a tool for water disposal
      autocontrol monitoring
     Disinfection methods: UV, ozone, chlorine,
      peroxides…


                  1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
Experiments with peracetic acid
                      100000


                       80000
                                                                                témoin
Taux d'ATP (en URL)




                                                                                bac traité à 5 mg/l
                       60000
                                                                                bac traité à 10 mg/l

                       40000


                       20000


                           0
                               0      10                 20                30                    40
                                             Temps (en heures)

                                   1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
Experiments with peracetic acid
6000

5000

4000

3000                                                               Before Hydrogent
                                                                   After Hydrogent
2000

1000

  0
         2.5ml    5ml        10ml       15ml       20ml
       - 51 %    - 66%     - 73 %     - 74 %        - 86 %

                         1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
Touchpool monitoring



                                             149 LRU




 87 LRU


          1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
            Surface disinfection




4 700 LRU       23 500 LRU




               1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
            Floor cleaning
25000

20000

15000
                                                          before cleaning
10000
                                                          after cleaning

5000

   0
        A     B             C     D (higner
                                concentration)



             1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
      Room disinfection (aerosol)
700

600

500

400                                                     before disinfection

300                                                     after peracetic acid
                                                        aerosol
200

100

 0
        A   B              C             D



                1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal
                    Conclusion

   Tool for microorganism level monitoring

     Rapid
     Easy to use
     Very useful




                1st AQUALITY Symposium, April 2 - 7, 2004, Oceanario de Lisboa, Portugal

								
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