University of Lethbridge Laboratory Safety Audit Checklist
Lab Supervisor: Date:
A. Documentation Yes No N/A Comments
1. Emergency contacts and procedures posted?
2. Laboratory Chemical Safety Manual available?
3. Chemical Spill Response Guideline available?
4. Chemical Inventory available and up to date?
5. MSDSs available for all controlled products?
6. Lab personnel aware of the location of the items
7. Lab personnel aware of the Working Alone Policy?
8. Lab personnel aware of the on-line Incident/Accident
9. WHMIS and Lab/Chemical Safety training records
10. Hazard assessments completed?
B. Housekeeping Yes No N/A Comments
11. Benches and sinks clean and tidy? Spills cleaned up
as soon as possible?
12. Exit doors unobstructed?
13. Aisles unobstructed?
14. Excessive combustible material (i.e. empty
packaging, boxes) present?
15. Tripping hazards (e.g. cords, hoses, equipment)?
16. Food or drink present in lab?
C. Emergency & Safety Equipment Yes No N/A Comments
17. Appropriate fire extinguisher(s) available?
18. Appropriate First Aid Kit available and fully stocked?
19. Safety glasses available and in use?
20. Lab coats and gloves worn?
21. Lab personnel wearing appropriate apparel?
22. Eyewash available and accessible?
23. Emergency shower available and accessible?
24. Maintenance log for testing eyewash and safety
shower available and up-to-date?
25. Appropriate Spill Kit available and fully stocked?
26. Appropriate safety carriers, carts, etc. available for
safe transport of chemicals?
27. Lab personnel aware of locations of PPE, First Aid
Kit, Spill Kit, fire extinguisher, safety showers,
D. Chemical Storage and Labelling Yes No N/A Comments
28. Chemicals segregated by compatibility
classification? For example:
Oxidizers separated from flammables/combustibles
Acids are separated from bases
Organic acids separated from inorganic acids
Nitric and /or perchloric acid separated from all other
Separate secondary containment available for
perchloric acid, hydrofluoric acid, and mercury.
Reactive substances are stored in an appropriate
location and manner. (i.e. water reactive substances .
stored in sealed secondary containers; air reactive
substances stored in an inert liquid, inert
atmosphere, or in a sealed secondary container).
D. Chemical Storage and Labelling (continued) Yes No N/A Comments
29. Storage locations of segregated chemical identified?
Flammable Liquids Spontaneously
Corrosive – Acids Combustible
Corrosive – Bases Water Reactive
Flammable Solids Highly Toxic*
30. Chemical containers have WHMIS compliant labels?
31. Chemicals are stored below eye level?
32. Glass vessels containing toxic, flammable,
combustible, or corrosive chemicals that pose an
inhalation hazard, stored in vented cabinets below
33. Chemicals dated upon receipt?
34. Peroxide forming chemicals labelled with date
container was opened?
35. Maximum allowable quantity of flammable liquids
stored in a flammable storage cabinet:
250L of Flammable or Class I Liquids; • up to 500L of
Combustible or Class II / III; for a combined total of no
more than 500L.
36. Volume of flammable liquids in open storage < 10L?
37. Flammables stored in intrinsically safe/explosion
proof refrigerator only (or fridge labelled “No
38. Flammable liquids not stored within 1.5m from the
exits in the lab?
39. All narcotics and ethanol (absolute and 95%) secured
and usage recorded in an inventory?
40. Chemical waste properly stored and labelled?
* Highly toxic is defined as substances that have a median lethal dose (LD50) of less than or equal to 50 mg/kg body weight by the oral route of
entry; 40 mg/kg body weight by the dermal route of entry,; or a median lethal concentration (LC50) less than or equal to 500 mg/m3 or 0.5 mg/l. It
is highly recommended that all “Highly Toxic” materials be kept in a locked cupboard, preferably a metal cabinet.
E. Fume Hoods Yes No N/A Comments
41. Fume hood flow monitor functioning correctly (green
light/indicator visible when sash is at 30-45cm)?
Proper face velocity is between 100-120 fpm at 30-
45cm sash height.
Exhaust fan #:
42. Fume hood information cards are up-to-date? Contact info:
Contact information, annual recalibration date, on 24 24 hour hood:
hours, local control, etc. Local control:
Annual recalibration date:
43. Fume hood clean and uncluttered?
Fume hoods shall not be used for long-term storage
44. Fume hood sash closed when not in use?
45. Fume hood sash kept between 30 – 45cm when in
46. Air baffles at back of hood blocked (apparatus not
closer than 3cm to the back of hood)?
47. Apparatus at least 15cm from front of hood?
48. Perchloric acid hood:
Maintenance log up-to-date?
Open containers of perchloric acid present?
49. Localized hoods/arms present and operational?
50. Vented storage cabinets available and functioning
F. Instrumentation Yes No N/A Comments
51. Instrumentation present in lab:
AA, GC, MS, IR, HPLC, low-temp freezers,
52. Does instrumentation require an exhaust hood or
any other specific engineering or PPE controls in
order to use it safely?
Yes No N/A Comments
G. Compressed Gas Cylinders
53. Gas cylinders upright and secured with non-
combustible restraint (chain)?
54. Compressed gas cylinders are not stored within 1m
from exits in the lab?
55. Empty cylinders clearing identified and stored
separately from full cylinder?
56. Cylinders not in use have caps in place?
57. Minimum number of gas cylinders stored in the lab?
58. Gas cylinders not in use segregated by class?
Toxic and corrosive gases must be separated from
flammable gases by 2m.
59. Appropriate types of gas regulators used?
An adapter must not be present between the
compressed gas cylinder and the regulator.
60. Appropriate handcart available for transporting
H. Other Yes No N/A Comments
61. Purge system:
Personnel know location of purge switch?
Signage is in place?
Switch locations are clearly marked?
62. Is the electrical panel accessible?
63. Are breakers clearly identified?