POSSIBLE PARTNERSHIP PROPOSAL
Dr. Cemil Göçmen, Çukurova University, Medical Faculty, Adana-TURKEY
RESEARCH INTERESTS : Basic concepts of environmental FP6 FOOD, 2nd Call
(with deadline February 2004)
Thematic Priority: Food Quality and Safety
Topic title: Mediterranean olives and grapes secondary metabolites and their impact on
Type of the instrument in this case:
Specific Targeted Research Projects (STREP)
Topic area: T6
Potential Impact: The protection of health by providing biological active foods to
humans. The project is concentrated on biologically active compounds (secondary
metabolites of olives and grapes; phenolic compounds) in olives and grapes. The
innovation is to extract phenolic compounds from olives and grapes, and to investigate
the pharmacological effects of the secondary metabolites of tese biological actice foods.
This project will include epidemiological studies on the influence of Mediterranean diet
and lifestyle on healty aging nor effects on humans.
Strategic objectives addressed
1. Phytochemical screening of Mediterranean olives and grapes samples involved in
2. Extraction of the secondary metabolites from the selected materials.
3. Fractionate and characterise antioxidant extract from olives and grapes.
4. Quantification of the active principles with a value for medicine.
5. Isolation and identification of new active compounds.
6. Characterisation of antitumoral effects
7. To investigate (in vitro and in vivo experimental condition) the impact of
Mediterranean olives and grapes secondary metabolites on diabetic, arteriosclerotic
and ageing animal models
8. Epidemiological studies on the influence of Mediterranean diet and lifestyle on healty
aging nor effects on humans
The consortium and project resources
Coordinator of the Turkish Group: Cukurova University, Faculty of Medicine,
Department of Pharmacology
General duty of the coordinator: Planning and organization of the project, following
the working of other participants, collecting, transferring and analysing data of
Name of the Coordinator of the Turkish Group:
Assoc. Prof. Dr. Cemil Gocmen, MD ( Male)
Address: Cukurova University, Medical Faculty, Department of Pharmacology 01330-
Tel: 00-90-322-3386060/ 3239-3240
Research Group Size:
4 professors (3 woman scientist); 2 associate professors; 1 assistant professors; 8 research
assistants (7 of whom are women); altogether 15 researchers.
In the department of pharmacology of University of Cukurova, there are two main
groups coordinated by Cemil Gocmen. The main interest of the first group is the smooth
muscle pharmacology in cardiovascular system, urogenital system and gastrointestinal
system. Their works are focused on nitrergic system, nitric oxide and S-nitrosothiols. In
the last years the attention has also been paid to the design of oxidative stress due to
diabetes and some divalent cations, and of new antioxidant therapy strategies on these
oxidative stress models. The group has the enough equipment to study in vitro and in
vivo pharmacological experiments. These includes isolated organ baths, isotonic,
isometric and pressure transducers, bronchospasm transducer, polygraphs, data
acquisition systems, perfusion and infusion pumps, cardio tacometer and the other
necessary equipment. In this department, it can be also measured the blood, bladder and
cavernosal pressure in anesthesized and conscious rats and mice. In conscious rats, it is
investigated the modulation of the micturiton reflex. And also, it is investigated the
relation between photorelaxation and nitric oxide with using ultraviolet lamps. In some
experiments, it is studied the effects of some drugs on nephrotoxicity induced by some
agents in rats. Also we have a calcium imaging system to measure the intracellular
calcium amount in cell or tissue cultures. We will be able to determine the intarcellular
calcium mobility in apopitotic cells. The main interest of the second group is
neuropharmacology. This group has focused their studies on to relationship of various
neurotransmitter system or ion-channels to behaviour and physiological functions. The
role of certain neurotransmitter systems on the analgesic, antidepressant, anticonvulsive
effects of drugs; the mechanism of alcohol dependence involving glutamatergic and
opiatergic systems; the contribution of potassium channels and nitric oxide on the
analgesic and antidepressant effects of drugs; the drugs affecting learning and memory
are ongoing research area of this group.
Experimental methods and research tools used by neuropharmacology group: Hot-plate,
tail-flick, abdominal constrictor tests for measuring analgesia, procedures for learning
and memory, electroconvulsive shock, foot shock, and cold stress methods, stereotaxic
applications, forced swimming test.
Laboratory resources available:
1. Behavioural studies: Analgasimeters, instruments for depression studies, Hamilton
syringes, activity meter, electroconvulsive shock apparatus, labirents for learning-
memory studies, stereotaxic apparatus.
2. In vitro pharmacological studies : _solated organ baths, student stimulator, electrodes,
isotonic or isometric transducers, polygraphs, data acquisition systems, UV lambs.
3. In vivo pharmacological studies: Perfusion pumps, infusion pumps, metabolic cages,
urinary bladder pressure transducer, intracavernosal pressure transducer, cardio
tacometer, blood pressure transducers, data acquisition systems
4. Additional resources available: Some chemicals, experimental animals (rat, mice and
5. Intracellular calcium imaging system (FURA-2)
Present project partners from Turkey:
From Cukurova University
A. Medical Faculty: 7 groups
Department of Medical Biology and Genetic
Department of Biochemistry
Department of Pathology
Department of Biophysics
Department of Urology
Department of Bioistatic
Department of Public Health
B. Food Engineering Faculty: 1 group
Present project partners from the other countries
Spain: 2 groups
Italy: 1 group
Possible Project Partners from the other countries: We are in contact.
Greece: 1 group
CZECH REPUBLIC: 1 group
Total: 5 countries 14 groups
Possible topics suggested to the Coordinator of the Consortium for the success of the
My team may contribute to the project by investigating the pharmacological effects of
polyphenolic compunds obtained from grape and olive on some pathological models in
some experimental animals.
We need some new partners who will be able to study on the Strategic objectives
Publications of the Turkish coordinator
1. Kansu Büyükaf_ar, Cemil Göçmen, Atilla Dikmen, Ergin _ingirik, Serpil Önder, Firuz
Baysal. Possible Nature of Mediator Responsible for Electrically-Induced relaxations in
the Longitudinal Muscle Preparation of Mouse Gastric Fundus. Annals of Medical
Sciences (1994) 3:39-44
2. Atilla Dikmen, Cemil Göçmen, Firuz Baysal, B.Faruk Erden, Sühendan Mavruk,
Serpil Önder, Ergin _ingirik. Effects of Lidocaine on Chemically and Electrically
Induced Seizures in the Mouse. Annals of Medical Sciences (1994) 3:64-70
3. C. Göçmen, P. Uçar, E. _ingirik, A. Dikmen, F. Baysal. An in vitro study of
nonadrenergic-noncholinergic activity on the cavernous tissue of mouse. Urol Res (1997)
4. A. Seçilmi_, _. Atcı, N. Yaktubay, Y. Karata_, C. Göçmen, E. _ingirik, A. Dikmen, F.
Baysal. The role of Na+ channels in twitch generation during exposure of the frog rectus
abdominis to Ca-free Ringer solution with Na2EDTA. Acta Med Okayama (1997) 51(3):
5. Cemil Göçmen, Ata Seçilmi_, Peyman Uçar, Yusuf Karata_, Serpil Önder, Atilla
Dikmen, Firuz Baysal. A possible role of S-nitrosothiols at the nitrergic relaxations in the
mouse corpus cavernosum. European Journal of Pharmacology (1998) 361;85-92
6. Kansu Büyükaf_ar, Emine Demirel-Yılmaz, Cemil Göçmen, Atilla Dikmen. Depletion
and restoration of the putative photosensitive materials store yielding nitric oxide in the
isolated mouse gastric fundus. The Journal of Pharmacology and Experimental
Therapeutics (JPET) (1999) 290;768-773.
7. K. Büyükaf_ar C. Göçmen, A. Seçilmi_, Y. Karata_, S. Göktürk, N._. Kalyoncu.
Evidence that the nitrergic neurotransmitter and endothelium-derived factor (EDRF)
might be S-nitrosothiols in the mouse corpus cavernosum. Acta Med Okayama (1999)
8. Ata Seçilmi_, I_ıl Öcal, Cemil Göçmen, Atilla Dikmen, Ergin _ingirik, Serpil Önder
and Firuz Baysal. Papaverine-induced and endothelium-dependent relaxation in the
isolated rat aortic strip. Acta Med Okayama (1999) 53; 171-177.
9. Y. Karata_, Y. Ergün, C. Göçmen, A. seçilmi_, E. _ingirik, A. Dikmen, F. Baysal.
Possible postsynaptic action of aminoglycosides in the frog rectus abdominis. Acta Med
Okayama (2000) 54:49-56.
10. C. Göçmen, E.K.Kumcu, A. Seçilmi_, P. Uçar, A. Dikmen, F. Baysal. Restoratif
effects of zinc and selenium on nitrergic relaxations impaired by cadmium in the mouse
corpus cavernosum. Toxicology Letters (2000) 111(3);229-234
11. C. Göçmen, A.Seçilmi_, E.K. Kumcu, P. Uçar, S. Önder, A. Dikmen, F. Baysal.
Effects of vitamin E and selenium on neurogenic and endothelial relaxation of corpus
cavernosum in the diabetic mouse. European Journal of Pharmacology (2000) 398:93-98.
12. Peyman Uçar, Cemil Göçmen, Ata Seçilmi_, Yusuf Karata_, Ergin _ingirik, Serpil
Önder, Atilla Dikmen and Firuz Baysal. Inhibitory Actions of Hydroxocobalamin,
Cyanocobalamin, and Folic Acid on the Ultraviolet Light-Induced Relaxation of Upper
Oesophageal Strips of Frog. Acta Med Okayama (2000) 54:147-52.
13. Göçmen C, Göktürk HS, Ertu_ PU, Önder S, Dikmen A, Baysal F. Effect of
neocuproine, a selective Cu(I) chelator, on nitrergic relaxations in the mouse corpus
cavernosum. Eur J Pharmacol. 2000 Oct 13;406 (2):293-300.
14. Peyman Uçar Ertu_, Kansu Büyükaf_ar, Eda Karabal Kumcu, Cemil Göçmen, ata
Seçilmi_, Ergin _ingirik, Atilla Dikmen, Firuz Baysal. Effects of some divalent cations
on nitrergic relaxations in the mouse corpus cavernosum. Fundamental & Clinical
pharmacology (2001) 343-348.
15. Y.Bayazıt, P.U. Ertu_, M. Ürünsak, C. Göçmen, I.A. Arıdo_an, T. Turunç, E.
_ingirik. Effects of chronic cadmium exposure on contractility of the rat detrusor muscle.
Urol Res (2002) In press.
16. Sefa Resim, H. Sinem Göktürk Büyüknacar, Cemil Göçmen, Serpil Önder, Atilla
Dikmen. A possible role of sulphydryl reagents on the tonic activity of the rat detrusor
muscle. Eur J Pharmacol, (2002) in press.