Microscope Tutorial Everything you probably didn‟t know you wanted to know, but now you will know, „ya know? Student Task • Please remove your microscope from the rear microscope cabinets. • Please be sure to get the correct „scopes for your group. – Ex: Group 4 from Cabinet Space #4. Key Microscope Parts: • Please have your microscope packet available for reference. Cornell Notes: Lesson Goals • Develop a complete understanding of microscope parts, their functions, and special details. • Become prepared to pass the Microscope Skills Test. Skills Test: What to Know • All microscope parts • All functions of each part • Proper movement of each part based on given directions. • Calculation of individual and total magnifications. • Understand the different effects produced by the iris diaphragm. • Proper procedure for mounting, viewing slides Please study the microscope picture and its parts in this slide as well as the next two slides. “Testing Your Understanding”: #1 • Is the microscope, seen in the previous three slides, properly prepared in order for storage in the Microscope Cabinet? • I look for four details to check if it is ready for storage. Microscope Storage Rules 1. Cord is loosely wrapped around the eyepiece. • “Lasso style” 2. No slide is found on the slide stage. 3. The (red) low power objective is pointing down. 4. The stage clip is centered so that the “elbow” does not “stick out” 5. Lastly, the microscopes are put into the proper cabinet space for their group. Stage Clip in the closed position Stage Clip in the open position Objective Lenses “The 411” “EW10XD” means the eyepiece lens magnifies by 10 X. “DIN 4 0.10” means the lens magnifies by 4 X. “DIN 10 0.25” means the lens magnifies by 10 X. “DIN 40 0.65” means the lens magnifies by 40 X. “DIN 100 1.25” means “Don’t Use!” “Testing Your Understanding”: 2 • You are looking at a slide at 4X (Low power with the red labeled objective lens). • What is the total magnification of the specimen on the slide? “And the answer is…” • 4X (obj. lens) X 10 X (eyepiece)= 40X total magnification Iris Diaphragm: Controlling Light and Detail Levels Iris Diaphragm Lever is nearly closed. Iris Diaphragm Lever Is fully open. Key Concept: Iris Diaphragm Iris Diaphragm Position Effect Mostly Closed Decreased Light (~90%) through the slide, greater detail in specimen. Mostly Open (~90%) Increased light through the slide, lesser detail in specimen. Knobs “What moves what…” Stage Control Knob: Moves the slide from back to front. Stage Control Knob: •Moves the slide from left to right. “Testing Your Understanding”: 3 • What two directions does the upper stage control knob move the stage clips? • What two directions does the lower stage control knob move the stage clips? “And the answer is…” • Upper stage control knobs: – Moves stage back and forth • Lower stage control knobs: – Moves stage left and right Coarse Adjustment Knob: Fine Adjustmen Used only for Low Knob: Power Objective •Used only for Medium & High Power Objective. •Used for small correcti to image at low powe Both sets of Fine/Coarse Adjustment knobs Work in the same way. Iris Diaphragm Control Knob: Not necessary to use How to Use Slides Procedure for viewing slides and actually seeing the correct thing! Slide Mount Technique 1. Obtain a prepared slide 1. These will usually be stored in a slide box. 2. Place the slide flat against the stage. 1. Be sure that the label is facing up. 3. Use the stage clip to “clamp” the slide between the silver “elbow” and the stage clip itself. 4. Using the stage control knobs, position the center of the slide beneath the low power objective. Slide Mount Technique (cont.) 5. Adjust the coarse adjustment knob in order to produce a clear image. 6. Move to next higher objective: Medium Power (10X). 7. Repeat steps 4-6. • Note: the only adjustment knob to be used at Medium and High Power is the FINE adjustment knob. Need I say anything about this part? “Testing Your Understanding”: 4 • While looking at a very transparent specimen, you find it difficult to see any details. • Please demonstrate how you can solve this problem using the microscope at your station/table. “And the answer is…” • Close down the iris diaphragm and increase the brightness of the lamp. Student Exit Task • Please copy the functions of each microscope part (from the white answer key) onto page one of your (student) Microscope packet.