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					Microscope Tutorial

Everything you probably didn‟t know
you wanted to know, but now you will
         know, „ya know?
Student Task

• Please remove your microscope
  from the rear microscope cabinets.
• Please be sure to get the correct
  „scopes for your group.
  – Ex: Group 4 from Cabinet Space #4.
Key Microscope Parts:

• Please have your microscope
  packet available for reference.
Cornell Notes: Lesson
Goals

• Develop a complete
  understanding of microscope
  parts, their functions, and special
  details.
• Become prepared to pass the
  Microscope Skills Test.
Skills Test: What to Know

• All microscope parts
• All functions of each part
• Proper movement of each part based on
  given directions.
• Calculation of individual and total
  magnifications.
• Understand the different effects produced by
  the iris diaphragm.
• Proper procedure for mounting, viewing slides
   Please study the
microscope picture and
 its parts in this slide
     as well as the
    next two slides.
“Testing Your Understanding”: #1


• Is the microscope, seen in the
  previous three slides, properly
  prepared in order for storage in
  the Microscope Cabinet?
• I look for four details to check if it
  is ready for storage.
Microscope Storage Rules

1. Cord is loosely wrapped around the
   eyepiece.
  •   “Lasso style”
2. No slide is found on the slide stage.
3. The (red) low power objective is pointing
   down.
4. The stage clip is centered so that the
   “elbow” does not “stick out”
5. Lastly, the microscopes are put into the
   proper cabinet space for their group.
Stage Clip in the
 closed position
Stage Clip in the
  open position
Objective Lenses


     “The 411”
 “EW10XD”
   means
the eyepiece
    lens
  magnifies
   by 10 X.
 “DIN 4
  0.10”
 means
the lens
magnifies
 by 4 X.
 “DIN 10
  0.25”
 means
the lens
magnifies
 by 10 X.
 “DIN 40
  0.65”
 means
the lens
magnifies
 by 40 X.
  “DIN 100
    1.25”
   means
“Don’t Use!”
“Testing Your Understanding”: 2


• You are looking at a slide at 4X
  (Low power with the red labeled
  objective lens).
• What is the total magnification of
  the specimen on the slide?
“And the answer is…”

• 4X (obj. lens) X 10 X (eyepiece)=

      40X total magnification
Iris Diaphragm:


Controlling Light and Detail Levels
Iris Diaphragm
      Lever
is nearly closed.
Iris Diaphragm
       Lever
   Is fully open.
Key Concept: Iris Diaphragm

  Iris Diaphragm Position         Effect

     Mostly Closed          Decreased Light
        (~90%)              through the slide,
                             greater detail in
                                specimen.
  Mostly Open (~90%)         Increased light
                            through the slide,
                             lesser detail in
                                specimen.
    Knobs


“What moves what…”
 Stage Control
     Knob:
Moves the slide from
   back to front.
                       Stage Control
                           Knob:
                       •Moves the slide
                              from
                          left to right.
“Testing Your Understanding”: 3


• What two directions does the
  upper stage control knob move the
  stage clips?
• What two directions does the
  lower stage control knob move the
  stage clips?
“And the answer is…”

• Upper stage control knobs:
  – Moves stage back and forth
• Lower stage control knobs:
  – Moves stage left and right
Coarse Adjustment
      Knob:           Fine Adjustmen
 Used only for Low         Knob:
  Power Objective      •Used only for Medium
                               & High
                           Power Objective.
                     •Used for small correcti
                        to image at low powe
Both sets of Fine/Coarse
   Adjustment knobs
 Work in the same way.
Iris Diaphragm
 Control Knob:
 Not necessary
      to use
How to Use Slides


Procedure for viewing slides and
actually seeing the correct thing!
Slide Mount Technique

1. Obtain a prepared slide
  1. These will usually be stored in a slide box.
2. Place the slide flat against the stage.
  1. Be sure that the label is facing up.
3. Use the stage clip to “clamp” the slide
   between the silver “elbow” and the
   stage clip itself.
4. Using the stage control knobs, position
   the center of the slide beneath the low
   power objective.
Slide Mount Technique (cont.)

  5. Adjust the coarse adjustment knob in
      order to produce a clear image.
  6. Move to next higher objective: Medium
      Power (10X).
  7. Repeat steps 4-6.
     • Note: the only adjustment knob to
        be used at Medium and High
        Power is the FINE adjustment
        knob.
Need I say anything
  about this part?
“Testing Your Understanding”: 4


• While looking at a very transparent
  specimen, you find it difficult to
  see any details.
• Please demonstrate how you can
  solve this problem using the
  microscope at your station/table.
“And the answer is…”

• Close down the iris diaphragm and
  increase the brightness of the
  lamp.
Student Exit Task

• Please copy the functions of each
  microscope part (from the white
  answer key) onto page one of your
  (student) Microscope packet.

				
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posted:5/8/2010
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