INTRODUCING PLANT TISSUE CULTURE IN THE CLASSROOM

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					INTRODUCTION TO PLANT TISSUE CULTURE
        Preliminary Workshop Program

                   Dr. Mike Kane
     Environmental Horticulture Department
     University of Florida, Gainesville, Florida
                 July 12 - 15, 2010
                INTRODUCTION TO PLANT TISSUE CULTURE

                           A FOUR-DAY WORKSHOP

                                 July 12 - 15, 2010

                               Dr. Mike Kane
                    Environmental Horticulture Department
                            University of Florida
                              Gainesville, FL


WORKSHOP OVERVIEW

The overall objective of the workshop is to provide instructional resources,
conceptual background information and hands-on laboratory experiences to
facilitate the incorporation of plant tissue culture (micropropagation) into
classroom curricula in the most cost efficient manner.

PARTICIPANTS

Primary focus is on grade school science, vocational agriculture and college
faculty. However, depending on availability of space, non-teachers wanting a
hands-on experience will be allowed to register.

INSTRUCTIONAL OBJECTIVES

Upon completion participants will:

   •   Understand the principles and concepts of plant tissue culture, specifically
       micropropagation
   •   Be familiar with the laboratory and greenhouse procedures and equipment
       used to propagate plants using micropropagation
   •   Have instructional materials including PowerPoint lectures, laboratory
       exercises and other informational resources that can be used in the
       classroom
   •   Know where to obtain supplies to economically and successfully teach
       plant tissue culture in the classroom
   •   Receive specialized, hands-on training completing several reliable
       laboratory exercises
   •   Observe the commercial application of the technology by visiting a
       commercial plant tissue culture laboratory
                                 PRELIMINARY PROGRAM

Monday, July 12, 2010

8:00-8:30 a.m.           Workshop Registration (Lobby Fifield Hall)

LECTURE SESSIONS (2316 Fifield Hall)

8:30 -8:40 a.m.          Welcome & Introductions

8:40 – 8:55 a.m.         Opportunities in Environmental Horticulture

8:55 – 9:00 a.m.         Workshop Goals & Objectives

9:00 – 9:30 am.          Plant Tissue Culture: A Historical Perspective

9:30 – 10:00 a.m.        Micropropagation Methods: Shoot Culture

10:00 – 10:20 a.m.       Break refreshments provided

10:20 – 11:00 a.m.       Micropropagation Methods: Shoot Culture (continued)

11:00 – 12:00 p.m.       Plant Tissue Culture: Instructional Resources

12:00-1:00 p.m.          Lunch provided


LAB SESSIONS (Building 68, In Vitro Plant Culture Lab)

1:00 – 2:30 p.m.         Laboratory Equipment and Sterile Technique

2:30 – 2:50 p.m.         Break Refreshments provided

2:50 – 5:30 p.m.         Starting Plant Tissue Cultures (Syngonium)

Dinner (on your own)


Tuesday, July 13, 2010

LECTURE SESSIONS (2316 Fifield Hall)

8:30 – 10:00 a.m.        Micropropagation Methods: Shoot Organogenesis and Non-zygotic
                         Embryogenesis

10:00 – 10:20 a.m.       Break refreshments provided

10:20 – 12:00 pm.        The Nuts & Bolts of Plant Tissue Culture Media

12:00 – 1:00 p.m.        Lunch Provided


LAB SESSIONS (Building 68, In Vitro Plant Culture Lab)

1:00 – 2:15 p.m.         Shoot Organogenesis – Parrot-feather

2:15 - 2:35 p.m.         Break refreshments provided in Building 68)

2:35 – 3:30 p.m.         In Vitro Flowering of Miniature Rose
3:30 - 5:00 p.m.           How to Prepare Plant Tissue Culture Media

6:15 – 8:00 p.m.           BBQ Dinner provided (Greenhouse Complex)


Wednesday, July 14, 2010

LAB SESSIONS               (Building 68, Plant in Vitro Culture Lab)

8:00 – 10:00 a.m.          Micropropagation Using Floral Tissues (Cauliflower)

10:00 – 10:20 p.m.         Break refreshments provided

10:20 – 12:00 p.m.         Using Commercially Available Plant Tissue Culture Kits

12:00 – 1:00 p.m.          Lunch provided

1:00 – 3:30 p.m.           Orchid Seed Culture

3:30 – 3:45 p.m.           Break refreshments provided

3:45 – 5:00 p.m.           Synthetic Seed Technology

Dinner (on your own)


Thursday, July 15, 2010

COMMERCIAL MICROPROPAGATION LABORATORY TOUR

8:00 a.m.                  Depart from in front of Fifield Hall (van provided)

10:15 – 12:00 p.m.         Commercial Tissue Culture Lab Tour, Apopka, Florida

12:00 – 1:30 p.m.          Lunch (on your own)

1:30 – 2:30 p.m.           Tour of UF Mid-Florida Research & Education Center (Tentative)

3:30 – 3:45 p.m.           Break

3:45 – 6:00 p.m.           Return to Gainesville, evaluation and adjourn



1
 If it is more convenient, some participants may want to drive their own vehicles to Apopka and depart for
home after the tour.
REGISTRATION INFORMATION

Enrollment in this workshop is limited to the first 16 participants, and registrations will be
accepted on a first-come, first-served basis (see registration form below). The $650
registration fee includes a CD-ROM containing Power Point lecture notes, images,
videotapes and laboratory exercises. Refreshment breaks and lunch (3 days) will be
provided for participants. Participants are responsible for making their own hotel
reservations (see below).

HOTEL ACCOMMODATIONS

Numerous hotels are available in the Gainesville area to provide guest room
accommodations during the workshop. Arrangements have been made for a
reduce rate at the Homewood Suites by Hilton (352-335-3133) which is located
within 2 miles of the University of Florida at 3333 SW 42rd Street, Gainesville, FL
32608 (see map). The rate of $89/night (not including local sales and occupancy
taxes) includes a hot continental breakfast and a welcome to the manager’s
reception light dinner (Monday – Thursday 5:30-7:30PM). Reservations must be
received June 25, 2010; thereafter reservations will be taken on a space
available basis only. When making your reservation please refer to the “Plant
Tissue Culture Workshop” to obtain the room rate.


CONTACT

Dr. Michael Kane, Professor
Environmental Horticulture Department
P.O. Box 110675
University of Florida
Gainesville, FL 32611-0675

Tel: 352-392-1831 ext. 205
Cell: 352-359-3565
Fax: 352-392-1413
E-mail: micropro@ufl.edu
http://hort.ifas.ufl.edu/
                                         REGISTRATION FORM




                         INTRODUCTION TO PLANT TISSUE CULTURE

                                          A Four-Day Workshop

                                             July 12 – 15, 2010

                                           University of Florida



Name: ______________________________________________________

Address: ____________________________________________________

          ____________________________________________________

Phone: _____________________________________________________

E-Mail: _____________________________________________________

Please include a check (credit cards not accepted) in the amount of $650 payable to:

University of Florida


Send registration form and payment to:

                             Dr. Michael Kane
                             Environmental Horticulture Department
                             P.O. Box 110675
                             University of Florida
                             Gainesville, FL 32611-0675


IMPORTANT NOTE:

Enrollment in this workshop will be limited to the first 16 paid registrants. Prior to submitting the registration
payment, please contact Dr. Kane either by email (micropro@ufl.edu) or telephone (office: 352-392-1831
ext. 205; cell: 352-359-3565) to verify that the workshop is not full. Registration fees are used to purchase
workshop materials. Please note that registration fees are non-refundable after Friday, June 9, 2010.
             UNIVERSITY OF FLORIDA AREA MAP
              Lectures will be held in 2316 Fifield Hall
           Laboratories will be held in Rm 111, Building 68




                                         Lecture
                                        2316 Fifield




                             IFAS Research
                                 D rive


                                                       Teaching Lab
                                                         Bldg. 68


Homewood Suites