Pharmacology I: CHEMICAL BIOLOGY 2008
Lectures: MWF, Sep 3 to Nov 12, 2008, 1:30 – 3:00 PM, Weill Auditorium
Course Directors Anthony Sauve aas2004@med.cornell.edu Derek Tan tand@mskcc.org Teaching Assistants Eleanor Allen eka2003@med.cornell.edu Elissaveta Petrova elp2003@med.cornell.edu
212-746-6224 646-888-2234
LC 216 ZRC 2165
212-746-6312 212-639-2551
LC216 RRL641
Grading 40% Problem Sets (due before class one week following each lecture) 20% Student Presentation 16% Examination #1 (Lectures 1–10) 24% Examination #2 (Lectures 11–25) Examinations closed book, in class 1) Wednesday, October 8, 1:30 – 3:00 pm 2) Wednesday, November 12, 1:30 – 3:00 pm Goals Students should develop an understanding of the chemical reactions used in biological and laboratory synthesis and manipulation of proteins, nucleic acids, lipids, and carbohydrates. Students should also develop an understanding for the chemical principles that underlie enzyme function. By the end of the course, students should be comfortable reading journal articles on chemical biology and will be expected to give an oral presentation and written report on a topic in the field. Lecture Schedule (** = class moved to A-250) Wed 9/3 Fri 9/5 Mon 9/8** 1. Reaction Kinetics 2. Thermodynamics 3. Receptor Binding Assays and Pharmacology Anthony Sauve Anthony Sauve Gavril Pasternak Jochen Buck Anthony Sauve Anthony Sauve David Gin David Gin Derek Tan
Wed 9/10** 4. Purification of Small Molecule Receptors Fri 9/12 Mon 9/15 Wed 9/17 Fri 9/19 Mon 9/22 5. Receptor–Ligand Interactions 6. Enzyme Kinetics 7. Organic Reaction Mechanisms I 8. Organic Reaction Mechanisms II 9. Primary Metabolite Synthesis I:
Peptides
Wed 9/24** 10. Primary Metabolite Synthesis II:
Oligonucleotides and carbohydrates
Derek Tan Samie Jaffrey Samie Jaffrey Samie Jaffrey Samie Jaffrey
Fri 9/26 Mon 9/29 Wed 10/1 Fri 10/3
11. Peptide Chemistry I:
Amino acid and peptide structure
12. Peptide Chemistry II:
Acidic residues
13. Peptide Chemistry III:
Basic residues
14. Peptide Chemistry IV:
Sulfur-containing residues
Mon 10/6** EXAMINATION #1: Lectures 1–10 Wed 10/8 Fri 10/10 Mon 10/13 Wed 10/15 Fri 10/17 Mon 10/20 Wed 10/22 Fri 10/24 Mon 10/27 Wed 10/29 Fri 10/31 Mon 11/3 Wed 11/5 Fri 11/7 Wed 11/12 15. Chemical Genetics and Drug Discovery I:
Fundamentals and techniques
Gabriela Chiosis Gabriela Chiosis Minkui Luo Derek Tan Derek Tan Derek Tan David Gin Yueming Li Yueming Li Luca Cartegni Scott Blanchard STUDENTS STUDENTS STUDENTS
16. Chemical Genetics and Drug Discovery II:
Case study
17. Chemical Genetics and Drug Discovery III:
Affinity-based chemical probes
18. Secondary Metabolite Synthesis I:
Non-ribosomal peptides
19. Secondary Metabolite Synthesis II:
Fatty acids and polyketides
20. Secondary Metabolite Synthesis III:
Steroids and terpenes
21. Glycopeptides and Glycoproteins
Structure, reactivity, and function
22. Proteases I:
Enzymology
23. Proteases II:
Mechanism-based inhibitors
24. Oligonucletide Chemistry I:
DNA structure and reactivity
25. Oligonucleotide Chemistry II:
RNA reactivity and enzymology
STUDENT PRESENTATIONS STUDENT PRESENTATIONS STUDENT PRESENTATIONS Examination #2: Lectures 11–25