BIO488L Medical Microbiology Lab Exercise 3

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					BIO488L: Medical Microbiology
       Lab Exercise 3



           Spring 2006
        T.A.: Sami Kemper
                   Today
●   Quiz 1
●   Expectations
●   Enterobacteriaceae Unknown Culture
    –   YOU WILL NEED TO CHECK THESE AT 24
        HOURS!!!!!!!
●   Clinical Unknown Culture
    Enterobacteriaceae Unknown
●   Record the letter of your unknown
●   Innoculate your enterotube
●   Check your results at 24 hours
●   Paper on the unknown is due in one week.
     –   Rubric
     –   Only one submission
                 Enterotube II
●   Combines multiple        –   Hydrogen sulfide
    biochemical tests            production
                             –   Indole production
●   Tests:
                             –   Adonitol, lactose,
    –   Glucose                  sorbitol and dulcitol
        fermentation (acid       fermentation
        or acid and gas)
                             –   Voges-Proskauer
    –   Lysine
        decarboxylase        –   Phenylalanine
                                 deaminase
    –   Ornithine
        decarboxylase        –   Urase
                             –   Citrate utilization
Innoculation of the Enterotube
Reading the Enterotube
             Clinical Unknown
●   2 organisms
●   Both must be accurately identified
●   You are responsible for determining what
    tests must be done and requesting them
●   Paper is due on the last day of wet lab
    (before presentations start)
    –   Rubric
    –   Only one submission
       Clinical Unknown Hints
●   Keep the culture pure!
●   Gram stain- with positivea nd negative
    controls on the other side of the slide
●   Think carefully about what tests you need
    to do and request them early
●   Take good notes