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Curriculum Vitae - College of Veterinary Medicine Cornell University

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Curriculum Vitae - College of Veterinary Medicine Cornell University Powered By Docstoc
					                                     Curriculum vitae
                                     November 2004

                                Sylvia J. Bedford-Guaus
                     Department of Veterinary and Animal Sciences
                    University of Massachusetts, 315 Paige Laboratory
                                  Amherst, MA 01003
                                 Phone#: (413) 545 5558
                           e-mail: sbedford@vasci.umass.edu

EDUCATION / CERTIFICATES

1999-2002   PhD, Department of Veterinary and Animal Sciences, University of
            Massachusetts. Thesis: Characterization of the Calcium Releasing Activity of
            Equine Sperm Extracts or Equine Sperm in Murine and Equine Oocytes:
            Implications in the Success of Equine Assisted Reproductive Techniques.

1997        Diplomate, American College of Theriogenologists.

1996        ECFVG (Educational Committee for Foreign Veterinary Graduates) Certification.

1991-1993   Master of Science Degree, College of Veterinary Medicine and Biomedical
            Sciences, Colorado State University. Thesis: Effect of seminal extenders
            containing egg yolk and glycerol on motion characteristics and fertility of stallion
            spermatozoa.

1986-1991   Licenciado en Veterinaria (Veterinary Medicine Degree; LV); Universitat
            Autònoma de Barcelona, Facultat de Veterinària, Barcelona, Spain.

PROFESSIONAL EXPERIENCE

2004        Assistant Professor, Department of Clinical Sciences, College of Veterinary
            Medicine, Cornell University, Ithaca, NY.

1998-2004   Lecturer, Department of Veterinary and Animal Sciences, University of
            Massachusetts, Amherst, MA.

1996-1998   Resident in Large Animal Reproduction, University of Pennsylvania, School of
            Veterinary Medicine, New Bolton Center, 382 West Street Road, Kennett Square,
            PA.

1994-1996   Resident in Large Animal Medicine, Tufts University, School of Veterinary
            Medicine, 200 Westboro Road, North Grafton, MA.

1993-1994   Intern in Theriogenology, Tufts University, School of Veterinary Medicine, 200
            Westboro Road, North Grafton, MA.
                                Curriculum vitae; Bedford; Page 2

OTHER APPOINTMENTS

2003-2004    Adjunct Assistant Professor, Department of Environmental and Population Health,
             Tufts University School of Veterinary Medicine, North Grafton, MA.

PROFESSIONAL ORGANIZATIONS

American College of Theriogenologists
American Association of Small Ruminant Practitioners
American Veterinary Medical Association
Society for Theriogenology

HONORS

2003          Snoeyenbose Award for Outstanding Graduate Student. Department of Veterinary
              and Animal Sciences, University of Massachusetts, Amherst.

1991          Scholarship given by a Spanish institution, "La Caixa"; this scholarship is
              awarded yearly to 60 students in all areas of study throughout Spain, to obtain
              postgraduate training in the United States.

RESEARCH GRANTS

2003          Healey Endowment Grant, University of Massachusetts. Title: In vitro conditions
              and molecular pathways conducive to equine sperm capacitation. PI: Sylvia J.
              Bedford. CoPI: Dr. Pablo Visconti. Amount: $14,400.

2000          Healey Endowment Grant, University of Massachusetts. Title: Characterization of
              the [Ca2+]i-releasing pattern of equine oocytes injected with stallion sperm or
              stallion cytosolic sperm extracts. PI: Sylvia J. Bedford. CoPI: Dr. Rafael A.
              Fissore. Amount: $9,450.

1997          Raymond Firestone Trust Research Grant, University of Pennsylvania. Title: The
              effects of egg yolk and cryopreservation on capacitation and acrosomal status of
              equine spermatozoa. PI: Sylvia J. Bedford. Faculty advisor: Dr. Stuart Meyers.
              Amount: $2,000.

1995          Massachusetts Horse Racing Research Fund Grant. Title: The effects of different
              capacitating media in the longevity of motility of stallion spermatozoa when
              incubated in capacitating conditions. PI: Sylvia J. Bedford. Faculty advisor: Dr.
              Katrin Hinrichs. Amount: $3,000.

VETERINARY LICENSES: Massachusetts, Pennsylvania (inactive).
                                Curriculum vitae; Bedford; Page 3

AD-HOC REVIEWER

American Journal of Veterinary Research
Biology of Reproduction
Journal of the American Veterinary Medical Association
Reproduction, Fertility and Development
Theriogenology

COMMITTEES

Professional College: Exam Committee for the American College of Theriogenologists

TEACHING EXPERIENCE

1998 - 2003

University of Massachusetts

Course organizer – Advanced Horse Management (AnSci 459 – 3-hr of lecture and 2-hr of
laboratories weekly). Course covers basic aspects of horse management including business,
facilities, nutrition, health management, reproduction, horse transportation and drugs that
enhance performance.

Course organizer – Horse Breeding Laboratory (AnSci 397A – 2-hr laboratories, three times
weekly). Advanced course in practical horse reproduction: Heat detection, mare breeding
management, foal watching, stallion handling, semen collection, and semen evaluation and
cooled/frozen storage.

Stud Farm Management (AnSci 445 – 9.5 hr of lecture). Lectures in breeding management of
the stallion, stallion subfertility, and assisted reproductive technology.

Animal Diseases (AnSci 372 – 13.5 hours of lecture; since Spring 2001). Lectures in swine,
small ruminant, South American camelids and equine diseases, emphasizing prevention of
disease from the management point of view.

Biotechnology (AnSci 200 – 1-hr lecture; Since Spring 2002). Lecture in advances of in vitro
assisted reproductive technology.

Animal Management II – (AnSci 252 – Horse Management Section – 3 hr of lecture and 10 hr of
practical laboratory per week; Spring 1999-2000). Five-week section in horse management,
including housing, nutrition, health management and common health problems, and
reproduction. .
                                  Curriculum vitae; Bedford; Page 4

Independent studies/Honor’s students:

       Spring 2003 – Six independent study students assisting with clinical reproductive cases at
our farm. Honor’s Thesis student starting research on incubation conditions and signaling
pathways conducive to stallion sperm capacitation.

        Spring 2002 – Honor’s Thesis student: Study on the effect of artificial vagina lubricants
on stallion sperm motion measures and pH.

        Spring 2001 - Four students assisted with horse breeding procedures at our equine
breeding facility (Hadley Farm), and designed and completed a study comparing the use of
different extenders to freeze stallion semen.

       Spring 2000 - Two students assisted with horse breeding procedures at the Hadley Farm.

       Spring 1999 – One student participated in horse management and assisted with treatments
and routine procedures on horses at the Hadley Farm. This student aided in setting up semen
freezing equipment at our breeding laboratory and running a stallion semen freezing trial.

Invited Lectures: Tufts University, School of Veterinary Medicine (1999-2003)

Reproductive Pathophysiology: Reproductive physiology of cats (1-hr), dogs (3-hr; since 2002)
and horses (3-hr).

Theriogenology - Breeding management and reproductive problems in cats (1-hr), dogs (6-hr)
and horses (6-hr; since 2002).

Invited Lectures: University of Pennsylvania, School of Veterinary Medicine (1998-2001)

Core Reproduction (VMED610 – formerly #7041; 2-hr lecture): Reproductive physiology and
pathology of South American camelids.

1996-1998

University of Pennsylvania, School of Veterinary Medicine

Core Reproduction (#7041; 3-hr lecture): Reproductive physiology of sheep, goats and llamas.

Large Animal Reproduction (#7542/7543; 3-hr lecture): Reproductive pathology and breeding
management of sheep, goats and llamas.

Senior Large Animal Reproduction Rotations (Spring and Summer): General clinical case
teaching and laboratories.
                                  Curriculum vitae; Bedford; Page 5

Large Animal Reproduction (#7542/7543) laboratories for third year veterinary students:
Reproductive ultrasonography, equine per rectum palpation and breeding soundness
examination, general reproductive pathology, cow palpation per rectum and speculum-vaginal
examination, bull, ram, stallion and boar breeding soundness examination.

Neonatology and Intensive Care Medicine (#7540; 1 hr): Problem solving session.

Principles of Medicine Laboratory (1 hr): Physical examination of llamas.

1993-1996

Tufts University, School of Veterinary Medicine

Theriogenology (8 hr): Breeding management of cats and South American camelids, and case-
based learning in stallions and female llama.

Reproductive Pathophysiology (3 hr): Reproductive physiology of cats, llamas and swine.

Large Animal Medicine (2 hr): Gastrointestinal pharmacology and examination of the patient for
gastrointestinal disease.

Clinical Pharmacology (1 hr): Antiparasitic drugs.

Theriogenology (16 hr; laboratories). Part of third-year Large Animal Medicine and Surgery
laboratories. Assist in teaching bovine obstetrics laboratory and equine breeding soundness
evaluation and palpation per rectum laboratory.

Clinical skills (5-15 hr; laboratories). Basic handling of horses and sheep, and general
management practices in sheep for first and second year veterinary students.

Senior Students Large Animal Medicine rotations (year-round). General clinical teaching.

1991-1993

Colorado State University

Equine Breeding Management (4-hr). Teaching assistantship in basic equine breeding
management for Equine Sciences students.

Short courses. Stallion semen collection and handling techniques (evaluation, cooling, freezing)
for horse breeders and practicing veterinarians.
                                 Curriculum vitae; Bedford; Page 6

TEACHING AT CONTINUING EDUCATION COURSES

2003 – Short Course for Breeders and Veterinarians in Stallion Management and Semen
Handling for Cooled/Transported Semen Programs; Equine Studies Program, University
of Massachusetts, Amherst, MA. January 25 and 26, 2003. Short course organizer. Lectures in:
Reproductive behavior problems in stallions; preparing stallion semen for cooled shipment; and
breeding the mare with cooled semen. One full day of laboratories teaching semen collection and
evaluation, and packaging for cooled transport.

2000 – I Congreso Internacional de Medicina y Cirugía Equina (I International Congress
in Equine Medicine and Surgery), Sevilla, Spain. November 22, 24 and 26, 2000. Laboratories
in: Breeding soundness examination in the mare; endometrial cytology, culture and biopsy;
presentation of clinical cases in mare reproductive pathology; and, discussion of hormonal
management of the estrus cycle in the mare. Lectures in: Hormonal control of the mare; clinical
monitoring of the estrus cycle in the mare; endometrial cytology, microbiology and biopsy; early
embryonic death and twin management; and, placental monitoring and pathology during
gestation.

1998 - Mid-Western Equine Breeding Management Workshop for Breeders and
Veterinarians, Gumz Farm, North Judson, IN. January 17 and 18, 1998. Lectures and
laboratories in: Basic reproductive anatomy and physiology of the mare; basic reproductive
anatomy and physiology of the stallion; semen collection methods; semen handling, semen
extenders and on-farm semen evaluation; the pre-season stallion breeding soundness
examination; transported (cooled) semen breeding programs; preparing semen for immediate
insemination and for cooled shipment; receiving cooled and frozen semen.

PRESENTATIONS

Research Presentations

11/17/04      Characterization of the [Ca2+] i releasing activity of equine sperm extracts and
              equine sperm in murine and equine oocytes: Implications in the success of equine
              assisted reproduction technologies. Reproductive Physiology/Endocrinology
              Seminar Series, Department of Biomedical Sciences, College of Veterinary
              Medicine, Cornell University, Ithaca, NY.

5/7/04        Evidence for [Ca2+]i Driven Apoptosis in In Vitro Aged Horse Oocytes. First
              Northeast Reproduction Meeting, Baker Institute, Cornell University, Ithaca, NY.

10/3/03       Oocyte maturation and effect on sperm or sperm extracts induced intracellular
              calcium oscillations. Havemeyer Foundation Workshop, Brewster, MA.

10/4/03       Media requirements for stallion sperm capacitation. Havemeyer Foundation
              Workshop, Brewster, MA.
                               Curriculum vitae; Bedford; Page 7

2/16/03     Characterization of the Calcium Releasing Activity of Equine Sperm Extracts or
            Equine Sperm in Murine and Equine Oocytes: Implications in the Success of
            Equine Assisted Reproductive Techniques. Invited seminar; Center for Germ Cell
            and Transgenesis, New Bolton Center, University of Pennsylvania, Kennett
            Square, PA.


7/25/02     Patterns of [Ca2+] i oscillations after intracytoplasmic sperm injection of in vitro
            and in vivo matured oocytes. Eighth International Symposium on Equine
            Reproduction, Colorado State University, Fort Collins, CO.

6/14/01     Activation and [Ca2+] i transients induced by microinjection of equine sperm
            cytosolic extract (eSF) on murine and equine oocytes. Research Seminar, Section
            of Reproduction, College of Veterinary Medicine, Texas A&M University,
            College Station, TX.

12/6/98     Semen, testicular volume, sperm production efficiency, and sexual behavior of
            stallions treated with trimethoprim-sulfametxazole and pyrimethamine. Annual
            Meeting, American Association of Equine Practitioners, Baltimore, MD.

7/13/98     Cryopreservation induces ‘capacitation-like’ changes in acrosomal status of
            stallion spermatozoa. Seventh International Symposium on Equine Reproduction,
            University of Pretoria, South Africa.

8/25/94     The effect of insemination volume on pregnancy rates of pony mares. Society
            for Theriogenology Annual Conference, Research Abstract Program, Kansas City,
            MO.

1/22/93     Effect of seminal extenders containing egg yolk and glycerol on stallion sperm
            motion characteristics and fertility. Thirteenth Equine Nutrition and Physiology
            Symposium, Gainesville, FL.

Posters

8/8-12/04   Localization of GM1 in non capacitated and capacitated equine sperm. Fifteenth
            International Congress in Animal Reproduction, Porto Seguro, Bahia, Brazil.

7/21/02     The effect of artificial vagina lubricants on stallion sperm motion measures and
            semen pH during cooled storage. Eighth International Symposium on Equine
            Reproduction, Colorado State University, Fort Collins, CO.

1/15/02     Activation and intracellular calcium ([Ca2+] i) transients induced by
            microinjection of equine sperm factor into murine and equine oocytes. Meeting
            for the International Embryo Transfer Society, Foz do Iguaçu, Parana, Brazil.
                              Curriculum vitae; Bedford; Page 8

Continuing Education Presentations

10/2/04     Other assisted reproduction techniques in the mare: Oocyte transfer and beyond.
            Equine Practitioners Conference, NY2K Veterinary Conference. Gideon Putnam
            Resort and Spa, Saratoga Springs, NY.

12/23/02    Progrés en reproducció assistida equina: Quan clonarem el primer cavall?
            (Progress in equine assisted reproduction: When will the first horse be cloned?
            Universitat Autònoma de Barcelona, Facultat de Veterinària, Bellaterra,
            Barcelona, Spain.

3/27/02     Advances in equine assisted reproductive technologies: Is the horse catching up?
            Meeting for Western Mass Veterinary Association, Easthampton, MA.

3/20/02     Research in reproduction and cloning in the horse. Dare-to-Dream Educational
            Farm Program for Youth. Orange, MA.

11/10/01    Embryo transfer and current research in assisted reproductive technology in
            mares. Equine Affair, Eastern States Exposition, West Springfield, MA.

10/10/01    Embryo transfer in mares. Second Annual Tufts Animal Expo, Hynes Convention
            Center, Boston, MA.

10/10/01    Breeding soundness examination of the mare. Second Annual Tufts Animal Expo,
            Hynes Convention Center, Boston, MA.

10/10/01    Breeding soundness examination of the stallion. Second Annual Tufts Animal
            Expo, Hynes Convention Center, Boston, MA.

11/10/00    Improving conception rates at breeding time. Equine Affair, Eastern States
            Exposition, West Springfield, MA.

11/9/00     Care of the late gestation mare and newborn foal. Equine Affair, Eastern States
            Exposition, West Springfield, MA.

9/30/00     Stallion behavior and management for breeding. University of New Hampshire,
            Equine Lecture series 2000, New London, NH.

4/5/00      Pregnancy and foaling. Seminar series for horse owners, Spring 2000. Equine
            Studies Program, Department of Veterinary and Animal Sciences, University of
            Massachusetts, Amherst, MA.
                                 Curriculum vitae; Bedford; Page 9
4/19/00       Colic and its prevention. Seminar series for horse owners, Spring 2000. Equine
              Studies Program, Department of Veterinary and Animal Sciences, University of
              Massachusetts, Amherst, MA.

11/11/99      Management of the pregnant and foaling mare. Equine Affair, Eastern States
              Exposition, West Springfield, MA.

3/13/96       Colic and its prevention. 2nd Annual Horse Symposium, West Lebanon, NH.

2/17/96       Breeding and foaling issues. 4th Annual Northeast Horsemen's Conference and
              Tradeshow, Boxborough, MA.

2/18/95       Transported semen in the horse: Basic techniques and mare management. 3rd
              Annual Northeast Horsemen's Conference and Tradeshow, Boxborough, MA.

1/18/95       Colic and its prevention. Equine health seminars; A Continuing Education
              Program for the Horse Owner. Tufts University School of Veterinary Medicine,
              North Grafton, MA.

7/16/94       Understanding your cat's estrus cycle. 11th Annual Feline Symposium for
              owners and breeders. Tufts University School of Veterinary Medicine, North
              Grafton, MA.

1/27/94       Pregnancy and foaling. 2nd Annual Northeast Horsemen's Conference and
              Tradeshow, Boxborough, MA.

1/26/94       Breeding management of the mare. 2nd Annual Northeast Horsemen's
              Conference and Tradeshow, Boxborough, MA.


PUBLICATIONS

Refereed journals

Bedford, S.J., Kurokawa, M., Hinrichs K., Fissore, R.A. Patterns of Intracellular Calcium
Oscillations in Horse Oocytes Fertilized by Intracytoplasmic Sperm Injection: Possible
Explanations for the Low Success of This Assisted Reproduction Technique in the Horse. Biol.
Reprod. 70:936-944 (2004).

Bedford, S.J., Kurokawa, M., Hinrichs, K., Fissore, R.A. Intracellular calcium oscillations and
activation in horse oocytes injected with equine sperm extracts or sperm. Reproduction 126:489-
499 (2003).

Bedford, S.J., Varner, D.D., Meyers, S.A. Effects of cryopreservation on the acrosomal status of
stallion spermatozoa. J. Reprod. Fert. Suppl. 56:133-140 (2000).
                                 Curriculum vitae; Bedford; Page 10

Bedford, S.J., McDonnell, S.M., Tulleners, E., King, D., and Habecker, P. Squamous cell
carcinoma of the urethral process as a cause of hemospermia in a stallion with self-mutilation
behavior. J. Am. Vet. Med. Assoc. 216(4):551-553 (2000).

Bedford, S.J., and McDonnell, S.M. Apparent experience-related aversion to handling for semen
collection in a stallion. Theriogenology Case of the Month. J. Am. Vet. Med. Assoc.
216(4):491493 (2000).

Bedford, S.J. and McDonnell, S.M. Measurements of reproductive function in stallions treated
with trimethoprim-sulfamethoxazol and pyrimethamine. J. Am. Vet. Med. Assoc. 215(9):1317-
1319 (1999).

Bedford, S.J., Gowdy, H.L. and Hinrichs, K. Comparison of longevity of motility of stallion
spermatozoa incubated at 38oC in different media and containers. Theriogenology 51(3):637-646
(1999).

Bedford, S.J., Hawes, M., Paradis, M.R., Mort, J.D. and Hinrichs, K. Peritonitis associated with
abdominal retention of the placenta in a llama. J. Am. Vet. Med. Assoc., 209(11):1914-1916
(1996).

Bedford, S.J., Jasko, D.J., Graham, J.K., Amann, R.P. and Squires, E.L. Effect of seminal
extenders containing egg yolk and glycerol on motion characteristics and fertility of stallion
spermatozoa. Theriogenology 43:955-967 (1995).

Bedford, S.J., Graham, J.K., Amann, R.P. and Squires, E.L. Use of two freezing extenders to
cool stallion spermatozoa to 5oC with and without seminal plasma. Theriogenology 42:571-578
(1994).

Bedford, S.J. and Hinrichs, K. The effect of insemination volume on pregnancy rates of pony
mares. Theriogenology 42:571-78 (1994).

Jasko, D.J., Bedford, S.J., Cook, N.L., Mumford, E.L., Squires, E.L. and Pickett, B.W. The
effect of antibiotics on motion characteristics of cooled stallion spermatozoa. Theriogenology
40:885-893 (1993).

Abstracts

Qin X, Dobarro M, Bedford-Guaus SJ, Ferris SP, Halperin JA. A role of complement in male
fertility: Targeted deletion of the complement regulatory protein CD59b results in progressive
loss of male fertility. Am J Reprod Immunol 49(6):348-349(2003).
                                 Curriculum vitae; Bedford; Page 11

Bedford SJ, Kurokawa M, Hinrichs K, Fissore RA. Patterns of [Ca2+] oscillations after
intracytoplasmic sperm injection of in vitro and in vivo matured horse oocytes. Abstract.
Proceedings 8th International Symposium on Equine Reproduction. Theriogenology 58:701-704
(2002).

Limone L, Shaughnessy D, Gómez-Ibáñez S, McDonnell SM, Bedford SJ. The effect of
artificial vagina lubricants on stallion sperm motion measures and semen pH during cooled
storage. Abstract. Proceedings 8th International Symposium on Equine Reproduction.
Theriogenology 58:333-336 (2002).

Bedford SJ, Kurokawa M, Hinrichs K, Fissore RA. Activation and intracellular calcium
([Ca2+]i) transients induced by microinjection of equine sperm factor into murine and equine
oocytes. Proceedings International Embryo Transfer Society, Theriogenology 57(1):699 (2002).

Bedford, S.J., and McDonnell, S.M. Semen, testicular volume, sperm production efficiency, and
sexual behavior of stallions treated with trimethoprim-sulfamethoxazole and pyrimethamine.
Abstract. Proceedings 44th Annual Convention Am. Assoc. Eq. Pract. (1998).

Bedford, S.J., Varner D.D., and Meyers S.A. Cryopreservation induces ‘capacitation-like’
changes in acrosomal status of stallion spermatozoa. Abstract. Proceedings 7th International
Symposium on Equine Reproduction (1998).

Bedford Guaus, S.J., Jasko, D.J., Graham, J.K., Amann, R.P. and Squires, E.L. Effect of
seminal extenders containing egg yolk and glycerol on stallion sperm motion characteristics and
fertility. Proceedings 13th Eq. Nutr. Physiol. Symp (1993).

Non refereed / Continuing education

Bedford, S.J. Breeding programs with cooled and frozen-thawed stallion semen. At:
http://PetPlace.com (2001).

Bedford, S.J. Reproductive physiology and breeding management of the mare. At:
http://PetPlace.com (2001).

Bedford, S.J. Care of the normal pregnant mare. At: http://PetPlace.com (2001).

Bedford, S.J. Breeding soundness examination of the mare. At: http://PetPlace.com (2001).

Bedford, S.J. Breeding soundness examination of the stallion. At: http://PetPlace.com (2001).

Bedford, S.J. Ultrasound for pregnancy diagnosis in the mare. At: http://PetPlace.com (2000).

Bedford, S.J. Semen collection from the stallion and handling for artificial insemination. At:
http://PetPlace.com (2000).
                          Curriculum vitae; Bedford; Page 12

Bedford, S.J. Review: Llama reproduction and breeding management. Society for
Theriogenology Newsletter, 18(1); (1995).

				
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