"Annual reports of OIE Reference Laboratories"
Bluetongue Dr Eileen N. Ostlund National Veterinary Services Laboratories, VS, APHIS, USDA P.O. Box 844, 1800 Dayton Avenue, Ames, IA 50010, USA Tel.: (1-515) 663-7551, Fax: (1-515) 663-7348 email@example.com, http://www.aphis.usda.gov/vs/nvsl Summary of general activities related to the disease 1 Test(s) in use/or available for the specified disease at your laboratory. Test For Specificity Total AGID Antibody Group 38 CELISA Antibody Group 927 CF Antibody Group 18 VN1 Antibody Type 25 Sheep Inoculation Antibody Group 298 Embryonated hen’s egg and BHK- Virus isolation Group 230 21 cell culture inoculation PCR Viral RNA detection Group 257 Typing PCR Differentiate BTV types 2,10, Type 7 11, 13 and 17 1 Each VN test represents neutralization assays against multiple bluetongue serotypes. 2. Production and distribution of diagnostic reagents AGID, CF, and CELISA diagnostic kits, licensed by the United States Department of Agriculture, are commercially available in the United States. Pre-license testing is conducted by the Center for Veterinary Biologics. Supplied nationally Supplied to OIE Reagent + Laboratory use Member Countries (units) (units) Strong positive ELISA/AGID BT antiserum (OIE) 1 3 Weak positive ELISA/AGID BT antiserum (OIE) 1 3 Bluetongue antibody proficiency panels 121 0 (20 samples/panel) Bluetongue virus antiserum for VN (various types) 37 4 Bluetongue virus (various types) 116 15 Bluetongue virus fluorescent antibody conjugate 32 0 Weak positive ELISA/AGID BT antiserum 63 2 Strong positive ELISA/AGID BT antiserum 8 2 Bluetongue virus RNA 34 0 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2007 1 Bluetongue Activities specifically related to the mandate of OIE Reference Laboratories 3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines Bluetongue AGID/CELISA proficiency panels are prepared and used in annual certification of approximately 60 laboratories in the United States. The panels are available to OIE Member Countries upon request. The laboratory participated in one international quality assurance BT antibody panel tests supplied by OIE countries 4. Preparation and supply of international reference standards for diagnostic tests or vaccines Strong and weak positive AGID/ELISA OIE reference standard serum is available. 5. Research and development of new procedures for diagnosis and control Reverse-transcriptase PCR methods were developed to identify isolates of bluetongue virus by serotype. The laboratory participated in validation studies of a real time reverse-transcriptase PCR diagnostic test for bluetongue 6. Collection, analysis and dissemination of epizootiological data relevant to international disease control Data on number, location (state), and serotype of U.S. bluetongue isolates are compiled and available upon request 7. Provision of consultant expertise to OIE or to OIE Member Countries Designated expert provided lectures on bluetongue for the Canadian Food Inspection Agency course on Foreign Animal Diseases. 8. Provision of scientific and technical training to personnel from other OIE Member Countries Laboratory assistance and training are available at the National Veterinary Services Laboratories. 9. Provision of diagnostic testing facilities to other OIE member countries None 10. Organisation of international scientific meetings on behalf of OIE or other international bodies None 11. Participation in international scientific collaborative studies The NVSL collaborated with the Institute for Animal Health, Pirbright, UK, in the molecular serotype identification of recent North American bluetongue isolates. Reference North American isolates were also submitted to the IAH for their international repository. 12. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences) ■ Presentations at international conferences and meetings Bluetongue activities at the NVSL. OIE Ad Hoc Group on Bluetongue. 12 – 14 March 2007. Teramo, Italy. This meeting concerned the establishment of a network of bluetongue reference laboratories. 2 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2007 Bluetongue Johnson DJ, Mertens PPC, Maan S, Ostlund EN. Exotic bluetongue viruses identified from ruminants in the southeastern USA from 1999-2006. Proceedings of the American Association of Veterinary Laboratory Diagnosticians, 50th Annual Conference Reno, NV, October 2007 Mertens PPC, Maan NS, Johnson DJ, Ostlund EN, Maan S: Sequencing and RT-PCR assays for genome segment 2 of the 24 bluetongue virus serotypes: Identification of exotic serotypes in the Southeastern USA (1999-2006). Proceedings of the 13th International World Association of Veterinary Laboratory Diagnosticians Melbourne, Australia, November 2007. Exotic bluetongue viruses identified from ruminants in the southeastern U.S. from 1999-2006. United States-Japan Cooperative Program in Natural Resources, Panel of Animal and Avian Health. 5 December 2007. Tsukuba, Ibaraki, Japan.. ■ Other communications CFIA (Canadian Food Inspection Agency)-USDA (United States Department of Agriculture) Meeting on Bluetongue. 12 June 2007. Ottawa, Canada. This meeting was held to discuss the identification of new U.S. serotypes of bluetongue virus and the possible impact to Canada, in particular, risk to livestock, trade issues, and bluetongue-free status. _______________ Annual reports of OIE Reference Laboratories and Collaborating Centres, 2007 3