Microsoft PowerPoint - Introduction to Molecular Biology by lindayy

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									Research Technologies
     Introduction
     Dr Philip Brewer
     CILR - UQ node
               Approach
                     Molecular
                      Biology



        Physiology                Cell Biology



                     Integrated
                       Biology



         Applied
                                   Genetics
         Biology



                      Plant
                     Anatomy




• Discovery Research
• Across species and across disciplines
                            Example Project

   Mutant defective             Find the gene
                                                           Apical bud                                   Pea
    in branching               that is defective           IAA
                                                                                         Bud
                                                                                       Outgrowth

                                                                        SMS Pathway            Reception &
                                                                                         RMS4 Local Signaling

                                                                        RMS1
                                                                        RMS5          RMS2   Feedback
Genetic
                                      What protein does                                       signal
pathway
                                       the gene make?
 model                                                    X-CK



                                                                                                     Shoot &
                   How does it                             Root                                     Rootstock
                  fit with other
                    proteins?                                           •Molecular Biology
                                                                        •Plant physiology
                                                                        •Computer modelling
             Tools
•   Lab species (“model plants”)
•   PCR
•   Bacterial vectors
•   Labelling
•   Microscopy
•   Transgenics
•   Large-scale arrays
•   Computer programming
 From DNA to RNA



<transcription movie from DNAinteractive DNA>
From RNA to Protein



<translation movie from DNAinteractive DNA>
          DNA extraction
• Break cells: release DNA

• Detergent: breaks down cell membranes

• Salt: dissociates DNA from associated proteins

• Alcohol: precipitates DNA

• Crystal violet stains DNA
            PCR



<PCR movie from DNAinteractive DNA>

								
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