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Microsoft PowerPoint - Introduction to Molecular Biology ...
Research Technologies Introduction Dr Philip Brewer CILR - UQ node Approach Molecular Biology Physiology Cell Biology Integrated Biology Applied Genetics Biology Plant Anatomy • Discovery Research • Across species and across disciplines Example Project Mutant defective Find the gene Apical bud Pea in branching that is defective IAA Bud Outgrowth SMS Pathway Reception & RMS4 Local Signaling RMS1 RMS5 RMS2 Feedback Genetic What protein does signal pathway the gene make? model X-CK Shoot & How does it Root Rootstock fit with other proteins? •Molecular Biology •Plant physiology •Computer modelling Tools • Lab species (“model plants”) • PCR • Bacterial vectors • Labelling • Microscopy • Transgenics • Large-scale arrays • Computer programming From DNA to RNA <transcription movie from DNAinteractive DNA> From RNA to Protein <translation movie from DNAinteractive DNA> DNA extraction • Break cells: release DNA • Detergent: breaks down cell membranes • Salt: dissociates DNA from associated proteins • Alcohol: precipitates DNA • Crystal violet stains DNA PCR <PCR movie from DNAinteractive DNA>
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