Docstoc

IACUC ANIMAL PROTOCOL REVIEW LIS

Document Sample
IACUC ANIMAL PROTOCOL REVIEW LIS Powered By Docstoc
					                             IACUC ANIMAL PROTOCOL REVIEW LIST
                                       December 2, 2005

Protocol #
05141          Glycemic Response to Test Fibers in Healthy Dogs
               5 Dog
                Class B
                amendment - The amendment asks for an additional dog to be added to the protocol.
                   One of the dogs currently on the protocol has become ill, is under veterinary care,
                   and is not cleared for use in research currently. By adding an additional animal, the
                   PI will be able to have adequate statistical power for his experiment. Once the ill dog
                   has recovered, it will be used as a potential replacement in case of another illness or
                   need to remove a dog from the study.
                Comments: none
                Stipulations: none
                Designated Reviewer Signature: _________________________________________
                Approval Date: _______________________________________________________
                Comments: __________________________________________________________
                   ___________________________________________________________________
                   ___________________________________________________________________

Protocol #
05186          Microinjection Into Zebra Fish Embryo
               10 Fish
                Class A
                Zebrafish (danio) eggs will be harvested and injected with silicon chips and silicon
                   microdevices. Viability and normal development will be monitored. Ten adult fish are
                   maintained in a 10-gallon aquarium. For breeding, the pair is placed in an 8x4x6-
                   inch plastic cage within the aquarium, allowing the eggs to drop through to a catch in
                   the bottom of the cage. No discomfort or illness is anticipated. Fish eggs are
                   undeveloped and have no pain receptors at the stage of implantation.
                Comments: The IACUC must inspect the housing area where the fish will be kept
                   before this project can proceed. COMPLETED 12/1/2005.
                Stipulations: The PI must forward copies of invoices whenever purchases of fish are
                   made so that DAR can record animal use.
                Designated Reviewer Signature: _________________________________________
                Approval Date: _______________________________________________________
                Comments: __________________________________________________________
                   ___________________________________________________________________
                   ___________________________________________________________________

Protocol #
05193          The Effect of Cotreatment with Hyaluronan and Corticosteroid on Equine Chondrocytes
               8 Horse
                Class A
                This is a renewal of a research protocol requesting the use of eight (8) horses.
                  Arthritis is a major cause of pain, dysfunction, and economic loss in horses. This
                  project seeks to use a cell culture system to establish a more comprehensive
                  analysis of hyaluronic acid (HA) and corticosteroid (CS) combinations in the
                  treatment of equine arthritis. Tissues will be sterilely collected from the joint surfaces
                  of horses that have been scheduled for euthanasia for reasons unrelated to this
                  project and unrelated to musculoskeletal disease. Chondrocytes will be isolated from
                  the joint surface and allowed to grow for one week in cell culture to form a cartilage
                  matrix. The chondrocytes will be supplemented with media containing no additives
                  (negative control), medium containing interluekin 1 (IL-1) (positive control), or
                  medium containing IL-1 combined with HA, CS, or HA and CS combined. A range of
                  HA and CS will also be utilized to mimic clinical therapy. The pellet cultures will be
                  evaluated for loss of proteoglycan, which is associated with early degenerative



IACUC Weekly Review List                   Page 1 of 3                                            12/2/2005
                   changes of the joint surface. This cell culture can also be standardized for a more
                   direct comparison between various drug combinations and concentrations without
                   sacrificing additional live animals. To date, there have been no sensitive, objective
                   evaluations of HA and CS used in combination for this disease process. The number
                   of horses needed is well justified and all horses would have been euthanized for
                   other reasons. In order to coordinate harvest of chondrocytes, horses may be held in
                   the Large Animal Clinic for up to 2-3 days.
                  Comments: The protocol utilizes tissues harvested from animals euthanized for non-
                   research purposes. The proposal is a sound study design that should be approved
                   without modification.
                  Stipulations: none
                  Designated Reviewer Signature: _________________________________________
                  Approval Date: _______________________________________________________
                  Comments: __________________________________________________________
                   ___________________________________________________________________
                   ___________________________________________________________________

Protocol #
05195          Animal Sciences 211
               90 Cattle 60 Pig 90 Sheep
                Class A
                Beef, swine, and sheep will be transported from the farms to the Stock Pavilion for
                   skills classes in animal evaluation for soundness, composition, and other phenotypic
                   characteristics used in market animal selection. Animals are transported to the
                   Pavilion by AnSci farm personnel following Ag Guide protocols. Two to four animals
                   will be used in each class session. Animals are kept in large viewing pens for
                   approximately two hours and are moved around by the instructor, then returned to
                   production facilities. Students do not handle the animals. No procedures are
                   performed. The approximate number of animals used (90 each beef and sheep, 60
                   swine) is the total for the three years the protocol is active.
                Comments: none
                Stipulations: none
                Designated Reviewer Signature: _________________________________________
                Approval Date: _______________________________________________________
                Comments: __________________________________________________________
                   ___________________________________________________________________
                   ___________________________________________________________________

Protocol #
05196          A Zebrafish Model of Lancefield Group C Streptococcal Pathogenesis
               400 Fish
                Class B
                This new research proposal will study Streptococcus pathogenesis in the zebrafish
                   model. The fish will be obtained from Dr. Kollmar's facility (UIUC) and taken in
                   plastic bags to 2614 VMBSB where they would be housed in one liter beakers of
                   aquarium salt solution for a 24-48 hour challenge period. The fish will be
                   anesthesized with MS-222 and the challenge will be delivered by IP and IM
                   injections. For IP injection anesthetized fish will be placed supine and supported by
                   the moistened gauze-covered open jaws of a hemostat.                   For IM injection
                   anesthetized fish will be positioned prostrate and supported with hemostats.
                   Following infection zebrafish will be housed in sterilized one liter beakers (six fish per
                   beaker). Beakers will be fitted with a perforated cover and housed in a 29°C
                   incubator with a transparent door and ambient light. IM-injected zebrafish will be
                   monitored three times daily until euthanasia (up to two days following injection) for
                   signs of abscessation, swelling, pallor, and any decrease in activity or range of
                   motion in the dorsal musculature. Zebrafish inoculated IP will be monitored every
                   two hours to detect early onset of systemic signs like lethargy, circling, or an inability
                   to maintain normal upright orientation. Euthanasia will be performed immediately



IACUC Weekly Review List                   Page 2 of 3                                             12/2/2005
                   upon the observation of circling or loss of righting ability. The fish will be euthanized
                   with an overdose of MS-222 after the post-challenge observation.
                  Comments: I'm not sure if this needs to go to full committee--it does involve pain
                   without drugs. Maybe the PI should provide a justification? Questions have been
                   answered to reviewers’ satisfaction.
                  Stipulations: none
                  Designated Reviewer Signature: _________________________________________
                  Approval Date: _______________________________________________________
                  Comments: __________________________________________________________
                   ___________________________________________________________________
                   ___________________________________________________________________

Protocol #
05197          The Influence of Habitat Fragmentation on Local Population Dynamics: An Experimental
               Approach
                Vole
                Class A
                The protocol is a renewal to complete a five-year study of the effect of habitat
                   fragmentation on populations of prairie voles. The effects of habitat fragmentation on
                   populations of wild animals are of great concern to conservation biologists because
                   ecological theory predicts that chances of extinction increase as habitat fragments
                   become smaller and more isolated. Prairie voles are trapped in six different
                   landscapes in March (lowest densities) and in November (highest densities) to
                   provide data on population fluctuations. Longworth live traps provided with food
                   (cracked corn) and bedding (cotton) are set on a Monday evening and serviced just
                   after sunrise and just before sunset each day through Saturday morning to reduce
                   the time remaining in the trap. Five days trapping allows the enumeration of nearly
                   every animal in a landscape, a necessary procedure to get an accurate estimation of
                   density. The metal traps have a board placed over them to reduce exposure to rain
                   and to radiative cooling and heating and are placed in cages to prevent disturbance
                   by raccoons. These procedures have allowed repeated capture of the same animals
                   without trap deaths. When first captured each animal is held by the nap of the neck
                   and marked with an ear tag that bears a unique number (animals neither flinch nor
                   vocalize during application of the tag). Each vole is then examined for reproduction
                   condition, molting, and presence of wounds, scars, or ectoparasites. After weighing
                   with a spring balance, they are released at the point of capture.
                Comments: none
                Stipulations: none
                Designated Reviewer Signature: _________________________________________
                Approval Date: _______________________________________________________
                Comments: __________________________________________________________
                   ___________________________________________________________________
                   ___________________________________________________________________




IACUC Weekly Review List                   Page 3 of 3                                            12/2/2005

				
DOCUMENT INFO