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Laboratory Waste Management Plan

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					Laboratory Waste Management Plan – June 2006                                                                                                    School of Biomedical Sciences

Waste Stream           Description                                    Collection/Treatment Procedures                                              Disposal Procedure
Non-hazardous          Consumable packaging and other                 Collect in a general waste bin lined with a black plastic bag.               The general waste bins are provided
waste                  materials that have not been                                                                                                and emptied by the University
                       contaminated with chemical, biological                                                                                      Cleaners.
                       or radioactive material.
                                                                                                                                                     Landfill

Biologically           Liquid tissue culture waste                    •    Decontaminate liquid culture media waste with high level                Once decontaminated, dispose of
contaminated                                                               hospital disinfectant eg Cavicide. Mix 1:1 and leave                    liquid waste down the sink with
waste                                                                      overnight.                                                              copious amounts of water.

                                                                                                                                                     Sewer

                       Solid waste contaminated with                  •    Collect in the provided containers (bin or wire basket) lined           Once autoclaved, Gautham to place
                       tissue culture, GMOs or associated                  with an autoclave bag.                                                  in YELLOW Clinical Waste bin for
                       low hazard chemical residues.                  •    Once full, seal the bag and transport to Micro prep room                collection by Stericorp.
                                                                           (L204) for autoclaving.
                       This includes used tissue culture flasks,                                                                                   Offsite Treatment   Landfill
                       plastic pipettes, gloves, and paper            Notes
                       towel etc.                                     •  Only staff/students trained by Gautham are to operate the
                                                                         autoclave.1
                       Note: For tissue culture contaminated          •  Secondary containment (eg. a locked clinical waste bin) is
                       with cytotoxic chemicals refer to                 required for all transport of biologically contaminated waste
                       cytotoxic waste                                   on campus.

                       Sharps including hypodermic needles,           •    Collect in an approved YELLOW Sharps container or                       Gautham to place in YELLOW
                       scalpel blades, pasture pipettes, slides            Qlicksmart scalpel blade removal system.                                Clinical Waste bin (at top of bin)
                       etc.                                           •    Once full, seal the container and leave in the Micro prep               for collection by Stericorp.
                                                                           room (L204) for disposal.
                                                                                                                                                     High Temperature Incineration

                       Pipette tips contaminated with                 •    Collect in sealed rigid container.                                      Gautham to place in YELLOW
                       biological material or chemical residue.       •    Once full, seal the container and leave in the Micro prep               Clinical Waste bin for collection by
                                                                           room (L204) for disposal.                                               Stericorp.

                                                                                                                                                   Offsite Treatment  Landfill
                                                                                                                                                   High Temperature Incineration




1
 Refer to the University’s Decontamination Guidelines for additional information on autoclave sterilisation cycles and monitoring procedures.
www.usyd.edu.au/ohs/biosafety/deconguidelines.shtml#2.1.2


                                                                                                                                                                              Page 1 of 4
Laboratory Waste Management Plan – June 2006                                                                                  School of Biomedical Sciences


Waste Stream          Description                              Collection/Treatment Procedures                                     Disposal Procedure
Chemically            Organic Solvents                         •   Collect in a labelled hazardous container indicating the type   Transfer to the Physiology Prep
Contaminated          eg, alcohol, hexane, xylene                  of solvent/s. 2 3 Ensure that incompatible solvents are         Room (L212), where they are stored
Waste                                                              collected in separate containers.4                              until collection (organised through
                                                               •   Transfer to the Physiology Prep Room (L212) for storage         Matthew Mitchell, OHS & Injury
                                                                   prior to collection.                                            Management Unit).
                                                               •   Lab Manager (Louise Hayes) to submit a Request for the
                                                                   Disposal of Hazardous Waste to the OHS & Injury                   Offsite treatment
                                                                   Management Unit (Fax 15868).                                      Reuse as fuel

                      Acid waste                               •   Collect in a labelled hazardous waste container indicating      Transfer to the Physiology Prep
                                                                   the type of acid and approximate concentration. Ensure          Room (L212), where they are stored
                                                                   that incompatible waste is collected in separate containers.    until collection (organised through
                                                               •   Transfer to the Physiology Prep Room (L212) for storage         Matthew Mitchell, OHS & Injury
                                                                   prior to collection.                                            Management Unit).
                                                               •   Louise Hayes to submit a Request for the Disposal of
                                                                   Hazardous Waste to the OHS & Injury Management Unit               Offsite Neutralisation
                                                                   (Fax 15868).                                                      Sewer

                      Empty Solvent Bottles                    •   Remove lid and dispose of as general waste.                     Place in general waste bins or glass
                                                               •   Evaporate the bottle dry in the fume cupboard.                  disposal bin in L212, K219 or H207
                                                               •   Remove or deface the label (eg. strike through with
                                                                   permanent pen).                                                   Landfill

                      Empty 200L & 20L metal drums             •   Remove bungs, and allow to evaporate dry. All drums must        Transport to SimsMetal
                      eg alcohol & methylated spirits              have two holes to ensure drainage or they will not be             recycled
                                                                   accepted for recycling by SimsMetal.
                                                               •   Leave drums outside, near L006                                  SimsMetal
                                                                                                                                   Scrap Metal Processing Centre
                                                               Notes                                                               125 Canterbury Rd, Bankstown
                                                               Dianne Borg (Anatomy) to book university ute with Andy              Phone 9709 6388
                                                               Galloway (Phone 19235) & organise drum transport.




2
    Ensure that all waste is labelled. Any unknown waste is charged a higher disposal fee.
3
    Waste containers &/or labels provided by technical staff on request.
4
    Refer to the University’s Guidelines for additional information on chemical incompatibility http://www.usyd.edu.au/ohs/ohs_manual/haz-subs/incompat.shtml


                                                                                                                                                               Page 2 of 4
Laboratory Waste Management Plan – June 2006                                                                         School of Biomedical Sciences


Waste Stream     Description                        Collection/Treatment Procedures                                     Disposal Procedure
Chemically       Low hazard liquid chemical waste   •   Collect in a labelled hazardous waste container indicating      Transfer to the Physiology Prep
Contaminated                                            the type of low hazard liquid waste.                            Room (L212), where they are stored
Waste                                               •   Louise Hayes to submit a Request for the Disposal of            until collection (organised through
                                                        Hazardous Waste to the OHS & Injury Management Unit             Matthew Mitchell, OHS & Injury
                                                        (Fax 15868).                                                    Management Unit).

                                                    Notes                                                                 Offsite Treatment
                                                    Ensure that all waste is labelled. Any unknown waste is charged       Sewer
                                                    a higher disposal fee.

                 Empty Containers of Solid          If non-hazardous, remove lid, rinse, deface label and dispose of    The general waste bins are provided
                 Chemicals                          as general waste.                                                   and emptied by the University
                                                                                                                        Cleaners.
                                                    If hazardous (refer to MSDS), seal the container.                      Landfill
                                                    •   Transfer to the Physiology Prep Room (L212) for storage
                                                        prior to collection.                                            If hazardous: Transfer to the
                                                    •   Louise Hayes to submit a Request for the Disposal of            Physiology Prep Room (L212),
                                                        Hazardous Waste to the OHS & Injury Management Unit             where they are stored until
                                                        (Fax 15868).                                                    collection (organised through
                                                            Description: Empty Chemical Packaging                       Matthew Mitchell, OHS & Injury
                                                                                                                        Management Unit).

                                                                                                                          Controlled Landfill

                 Batteries                          •   Transfer to the Physiology Prep Room (L212) for storage         Transfer to the Physiology Prep
                                                        prior to collection.                                            Room (L212), where they are stored
                                                    •   Louise Hayes to submit a Request for the Disposal of            until collection (organised through
                                                        Hazardous Waste to the OHS & Injury Management Unit             Matthew Mitchell, OHS & Injury
                                                        (Fax 15868).                                                    Management Unit).

                                                                                                                          Offsite treatment
                                                                                                                          Landfill

Clinical Waste   Microbiological Plates & Blood     •   Leave in the Micro prep room (L204) for disposal.               Gautham to put in YELLOW
                 Products                                                                                               Clinical Waste bin for collection by
                                                                                                                        Stericorp

                                                                                                                          Offsite treatment
                                                                                                                          Landfill




                                                                                                                                                   Page 3 of 4
Laboratory Waste Management Plan – June 2006                                                                              School of Biomedical Sciences


                  Animal Carcasses                       •   Place in prep room freezer L211 or chest freezer in L209.        Gautham to put in PURPLE
                                                         •   Gautham to organise disposal with cytotoxic waste.               Cytotoxic Waste bin for collection
                                                                                                                              by Stericorp.
                                                         Notes
                                                         DO NOT put in YELLOW Clinical Waste bin                                Incineration

Cytotoxic Waste   Sharps used in association with        •   Collect in an approved PURPLE Cytotoxic Sharps                   Gautham to put in PURPLE waste
                  cytotoxic drugs (eg. cisplatin)            container.                                                       bin for collection by Stericorp.
                                                         •   Once full seal container and transfer to the Micro Lab (L204)
                                                             for storage prior to collection.                                   High Temperature Incineration

                  Solid Waste contaminated with          •   Collect in the PURPLE cytotoxic bucket                           Gautham to put in PURPLE waste
                  cytotoxic drugs or Ethidium Bromide,   •   Once full seal bucket and transfer to the Micro Lab (L204)       bin for collection by Stericorp.
                  including contaminated agarose and         for storage prior to collection.
                  acrylamide gels and consumables                                                                               High Temperature Incineration

                  Cytotoxic Tissue Culture Waste         •   ALL tissue culture waste that is cytotoxic should be collected   Gautham to put in PURPLE waste
                  (solid)                                    in an autoclave bag.                                             bin for collection by Stericorp.
                                                         •   Transfer to the Micro Lab (L204) for autoclaving prior to
                                                             collection. Please ensure that the waste is clearly labelled.      High Temperature Incineration

                  Liquid Ethidium Bromide Waste –        •   Use activated charcoal teabag to deactivate                      Once deactivated, dispose of liquid
                  buffers contaminated with Ethidium     •   Leave overnight.                                                 waste down the sink with copious
                  Bromide.                               •   Dispose of down sink.                                            amounts of water.

                                                         Notes                                                                  Sewer
                                                         See Dr Diana Oakes for more information




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Description: Laboratory Waste Management Plan