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Osmosis and Tonicity


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									Osmosis and Tonicity

Key terms/concepts: Osmosis, turgidity, water potential, solute concentration, hypotonic,
hypertonic, isotonic, plasmolysis.

Objective: To determine the solute concentration of potato tuber tissue by interpolation of data.

      Potatoes (tubers from Solanum tuberosum)
      Sucrose solutions: 0.2, 0.4, 0.6, 0.8, and 1.0 M (molar) solutions
              * A 1.0 molar solution is one gram molecular weight per liter. With
                sucrose, the molecular weight is 342 (C12H22O11), so a 1.0 molar
                solution of sucrose is 342 grams in a liter (total volume w/ solute) of water. The
                other concentrations are adjusted proportionally.
      Distilled water
      250 ml Beakers

Procedure: Day 1
     Cut six „French fry‟ sections of potato to 5 cm length.
     Rinse the cut potatoes and allow them to drain on a paper towel for 60 seconds.
     Mass the potatoes on a small piece of aluminum foil to the nearest 0.1 gram.
     Put the pieces (all 6) in a beaker in 150 ml of one of the six concentrations of
            sucrose as follows: Station 1 - DW; Station 2 - 0.2 M; Station 3 - 0.4 M;
            Station 4 - 0.6 M; Station 5 - 0.8 M; Station 6 - 1.0 M.
     Leave the potato pieces in the respective solutions overnight...covered with
            plastic wrap (label masking tape with group information and put it on the wrap).

              Day 2
       Remove the potato pieces and drain them on a paper towel for 60 seconds, then mass
              them again to the nearest 0.1 gram.
       Calculate % change in mass (M) = MF - MI divided by MI) x 100.
       Record your group‟s % M to one decimal on the table on the board. We will use data
       from 3 classes to replicate the experiment 3 times.

Graphical Analysis
       Plot the six points on a graph. “Percent change in mass as a function of solute
       concentration” Draw a regression line to represent the data.
       Determine whether each of the six liquids are hypo-, iso-, or hypertonic to the cells in the
       Determine the solute concentration of the potato from your graph by interpolating from
the point where the regression line intersects Y = 0 (the concentration where the change in
mass was zero).
       The point on your graph where the regression line intersects Y = 0 corresponds to the
concentration (X) that best represents the solute concentration of the tuber tissue. Determine
this concentration in moles.

Note that there are both positive and negative values for Y (% M).

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