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					                               CURRICULUM VITAE

                             SAURABH KULSHRESTHA




Office:                                    Residence:
Department of Life Science                 BF-21
National Institute of Technology           National Institute of Technology Campus
Rourkela – 769008                          Rourkela – 769008
Orissa                                     Orissa
India                                      India
Phone Numbers:                             Email id:
+91-661-2462683 (Off)                      saurabhk@nitrkl.ac.in
+91-661-2463683 (Res)                      saurabh_kul2000@yahoo.co.in

Date of Birth:        July 19, 1978
Marital Status:       Married
Sex:                  Male
Nationality:          Indian

Education:

    B. Sc. (Biochemistry) Ist division in 1998 from Kurukshetra University
     Kurukshetra
    M. Sc. (Biochemistry) Ist division in 2000 from Kurukshetra University
     Kurukshetra
    Ph.D. * (Bioscience) in 2005 from Jamia Millia Islamia, New Delhi [Worked at
     Institute of Himalayan Bioresource Technology (CSIR) Palampur]

* Title of Ph.D. Thesis: Molecular characterization of the virus causing rose mosaic
                        disease complex

Total years of research experience:   8 year
Total years of teaching experience:   6 months

Other Qualifications/Certifications/Programs attended:
    Postdoctoral Associate (Plant-Microbe interaction) from July 2008 – March 2009
      at Department of Plant-Microbe Interaction, Max-Planck Institute for Plant
      Breeding Research, Köln, Germany
    Postdoctoral Associate (Plant Pathology, host pathogen interaction) from
      December 2005 – October 2007 at Department of Plant Pathology, University of
      Kentucky, USA
    GATE - 2000 qualified with 90.99 percentile
    Awarded CSIR-HRD Senior Research Fellowship in year 2003
    Attended a short-term training program on “Principles and uses of sophisticated
     instruments in biological Sciences” from May 24-27, 2004 at CPRI, Shimla,
     India.
    Annual group member of American Phytopathological Society for the years 2003
     & 2004
    Annual student member of British Society of Plant Pathology for the year 2004
    Annual member of Indian Phytopathological Society
    Annual member of Electron Microscope Society of India

Experience:

March 2009 – Present at Department of Life Science, National Institute of
Technology, Rourkela, Orissa.
Job Profile: Lecturer
Presently engaged in starting up of new department (Department of Life Science), it will
offer M. Sc. in Life Science. The department will be starting its first batch in July 2009.
Also engaged in writing up grant proposal to apply in different funding agencies to
develop my own research lab.

July 2008 – March 2009 at Department of Plant Microbe Interaction, Max-Planck
Institute for Plant Breeding Research, Köln, Germany.
Job Profile: Research (Postdoctoral Associate).
Worked on functional characterization of candidate pathogenicity genes from
Colletotrichum higginsianum through random insertional mutagenesis; generation of
targeted knock-out; over-expression mutants and expression analysis. Generated mutants
strains of C. higginsianum deficient in beta importin and ATP binding endoribonuclease
gene. Both the mutant strains were less pathogenic to Arabidopsis and initial results
showed that beta importin is playing role in fungal switch from biotrophy to necrotrophy.

November 2007-April 2008 at Department of Biotechnology, Faculty of Engineering
and Technology, Raja Balwant Singh College Agra
Job Profile: Lecturer
I worked as lecturer (Biotechnology) at the Faculty of Engineering & Technology, Raja
Balwant Singh College, Agra (Affiliated to UPTU Lucknow). I taught Genetics and
Molecular Biology, Genetic Engineering, Intellectual Property rights and
Immunodiagnostics courses at B. Tech Level.

December 2005-October 2007 at Department of Plant Pathology, University of
Kentucky, USA
Job Profile: Research (Postdoctoral Associate)
I tested the hypothesis that changes in levels of glycerol, or its products, resulting from
pathogen metabolic activity represents a novel signal triggering defense responses in
Arabidopsis. Our approach was to manipulate glycerol metabolic genes in Arabidopsis
and in Colletotrichum higginisianum, cause of crucifer anthracnose.
Achievements: Using Glycerol 3 phosphate dehydrogenase knockout (g3pdh) of C.
higginsianum and different glycerol metabolic mutants of Arabidopsis, we demonstrated
that glycerol 3 phosphate (G3P) is playing key role in conferring resistance in
Arabidopsis to C. higginsianum (One research paper published in Plant Physiology,
one in Fungal genetics and Biology and one paper is under final stages of
preparation for Molecular Plant Microbe Interaction).

June 2003-November 2005 at Institute of Himalayan Bioresource Technology
(CSIR), Palampur, India
Job Profile: Research and completed Ph.D. (Senior Research Fellow)
Worked as Senior Research Fellow (CSIR-HRD SRF) on “Molecular Characterization of
the Virus Causing Rose Mosaic Disease Complex” I have also completed the Ph.D.
degree under the same topic. In this I have developed diagnostic protocols (ELISA & RT-
PCR based) for the detection of virus infecting rose, I have characterized complete Coat
Protein (CP), Movement Protein (MP) and RNA 3 of rose isolate of Prunus necrotic
ringspot virus (PNRSV). The CP gene of this isolate was expressed in E.coli and
recombinant protein was purified and antibody was developed for Indian isolate of
PNRSV. Domains of CP gene of PNRSV were identified for interaction among
themselves using yeast two hybrid system. I also used to take theory and practical classes
(Molecular Virology) of M.Sc. Biotechnology students of CSKHPKV Palampur.
Achievements: Sequenced rose isolate of PNRSV, developed ELISA based diagnostic
kit for the detection of PNRSV, study cp-cp interaction in PNRSV, published several
research papers in impact factor journals and filed patent for the PNRSV diagnostic kit.

October 2000-June 2003 at Institute of Himalayan Bioresource Technology (CSIR),
Palampur, India
Job Profile: Research (Junior Research Fellow)
Worked as Junior Research Fellow (JRF) in a Department of Biotechnology, Govt. of
India, funded Project entitled “National Facility for Virus Diagnosis and Quality Control
of Tissue Culture Raised Plants” on Development of ELISA and PCR based diagnostics
of viruses infecting Ornamental Plants. I also used to take theory and practical classes
(Molecular Virology) of M.Sc. Biotechnology students of CSKHPKV Palampur.
Achievements: Developed ELISA and PCR based diagnostic protocols for the detection
of several ornamental viruses, Sequence several genes and genomes of viruses infecting
ornamental crops, developed ELISA based diagnostic kits for the detection of Carnation
mottle virus (CarMV) and Carnation etched ring virus (CERV), published several
research papers in international and impact factor journals and filed patent for the
detection of CarMV and CERV.

PUBLISHED PAPERS

    1. Kulshrestha, S., Hallan, V., Raikhy, G. and Zaidi, A. A. (2009). Potential uses
       of in vitro expressed and purified recombinant Prunus necrotic ringspot virus
       coat protein gene. Archives of Phytopathology and Plant Protection 42(5): 442-
       452.
2. Chanda, B., Venugopal, S. C., Kulshrestha, S., Navarre, D. A., Downie, B.,
   Vaillancourt, L., Kachroo, A. and Kachroo P. (2008). Glycerol-3-phosphate
   levels are associated with basal resistance to the hemibiotrophic fungus
   Colletotrichum higginsianum in Arabidopsis. Plant Physiology 147: 2017- 2029.
   (Impact Factor: 6.367).

3. Venard, C., Kulshrestha, S., Sweigard,. J., Nuckles, E. and Vaillancourt, L.
   (2008). The role of a fadA orthologue in the growth and development of
   Colletotrichum graminicola in vitro and in planta. Fungal Genetics and Biology
   45: 973-983. (Impact Factor: 3.425).

4. Raikhy, G., Hallan, V., Kulshrestha, S. and Zaidi, A. A. (2007). Polyclonal
   antibodies to the coat protein of Carnation etched ring virus expressed in
   bacterial system: Production & use in immunodiagnosis. Journal of
   Phytopathology 155: 616-622. (Impact Factor: 0.896)

5. Kulshrestha, S., Hallan, V., Raikhy, G., Ram, R. and Zaidi, A. A. (2006).
   Incidence of Bean yellow mosaic virus on Iris. Acta Horticulturae 722: 235-240.

6. Kulshrestha, S., Hallan, V., Raikhy, G., Ram, R., Garg, I. D., Haq, Q. M. R. and
   Zaidi, A. A. (2006). Occurrence of Iris mild mosaic potyvirus in cultivated iris in
   India. Indian Journal of Biotechnology 5:94-98.

7. Verma, N., Kumar, K., Kulshrestha, S., Raikhy, G., Hallan, V., Ram. R. and
   Zaidi, A. A. (2006). Detection and molecular characterization of a Tomato
   aspermy virus isolate infecting Chrysanthemums in India. Acta Horticulturae
   722: 41-53.

8. Raikhy. G., Hallan, V., Kulshrestha, S., Sharma, M. L., Verma, N., Ram, R. and
   Zaidi, A. A. (2006). Detection of Carnation ringspot and Carnation vein mottle
   viruses in carnation cultivars in India. Acta Horticulturae 722: 247-258.

9. Chandel, V., Kulshrestha, S., Hallan, V. and Zaidi, A. A. (2006). Natural
   infection of Ornithogalum mosaic virus on Iris from India. Plant Pathology 55:
   284. (Impact Factor: 2.012).

10.Raikhy, G., Hallan, V., Kulshrestha, S., Ram, R. and Zaidi, A. A. (2006).
   Complete nucleotide sequence of an Indian isolate of Carnation etched ring virus
   and its homology with other caulimoviruses. Current Science 90(2): 176-187.
   (Impact Factor: 0.800).

11.Raikhy, G., Hallan, V., Kulshrestha, S., Ram, R. and Zaidi, A. A. (2006).
   Multiplex PCR and genome analysis of Carnation mottle virus Indian isolate.
   Current Science 90(1): 74-82. (Impact Factor: 0.800).
12.Kulshrestha, S., Verma, N., Hallan, V., Raikhy, G., Singh, M.K., Ram, R. and
   Zaidi, A. A. (2005). Detection and identification of Prunus necrotic ringspot
   virus in Pelargonium. Australasian Plant Pathology 34: 599-601. (Impact
   Factor: 0.472).

13.Kulshrestha, S., Hallan, V., Raikhy, G., Adekunle, O.K., Haq, Q. M. R., and
   Zaidi A. A. (2005). Reverse transcription polymerase chain reaction-based
   detection of Arabis mosaic virus and Strawberry latent ringspot virus in vector
   Nematodes. Current Science 89(10): 1759-1762. (Impact Factor: 0.800).

14.Adekunle, O. K., Kulshrestha, S., Prasad, R., Hallan, V., Raikhy, G., Verma N.,
   Ram, R., Kumar, S. and Zaidi, A. A. (2005). Plant parasitic and vector nematodes
   associated with Asiatic and Oriental hybrid lilies. Bioresource Technology
   97(3): 364-371. (Impact Factor: 3.103).

15.Kulshrestha, S., Mehra, A., Hallan, V., Raikhy, G., Ram, R. and Zaidi, A. A.
   (2005). Molecular evidence for the occurrence of Tuberose mild mottle virus
   infecting tuberose (Polianthes tuberosa) in India. Current Science 89(5): 870-
   872. (Impact Factor: 0.800).

16.Sharma, A., Mahinghara, B. K., Singh, A. K., Kulshrestha, S., Raikhy, G., Singh,
   L., Verma, N., Hallan, V., Ram, R. and Zaidi, A. A. (2005). Identification,
   detection and frequency of lily viruses in northern India. Scientia Horticulturae
   106(2): 213-227. (Impact Factor: 0.694).

17.Singh, H. P., Hallan, V., Raikhy, G., Kulshrestha, S., Sharma, M. L., Ram, R.,
   Garg, I. D. and Zaidi, A. A. (2005). Characterization of an Indian isolate of
   Carnation mottle virus infecting carnations. Current Science 88(4): 594-601.
   (Impact Factor: 0.800).

18.Verma, N., Singh, A. K., Singh, L., Raikhy, G., Kulshrestha, S., Singh, M. K.,
   Hallan, V., Ram, R. and Zaidi, A. A. (2005). Cucumber mosaic virus (CMV)
   infecting Alstroemeria hybridus in India. Australasian Plant Pathology 34: 119-
   120. (Impact Factor: 0.472).

19.Verma, N., Singh, L., Singh, A. K., Kulshrestha, S., Raikhy, G., Hallan, V.,Ram,
   R. and Zaidi, A. A. (2005). Ornithogalum: a new host of Cucumber mosaic virus
   (CMV) from India. Plant Pathology 54: 256. (Impact Factor: 2.012).

20.Kulshrestha, S., Hallan, V., Raikhy, G., Kumar, A., Ram, R., Garg, I. D. and
   Zaidi, A. A. (2004). Molecular Characterization of Iris severe mosaic virus
   (ISMV) Isolate from India. Acta Virologica 48(1): 65-67. (Impact Factor:
   0.560).

21.Verma, N., Singh, A. K., Singh, L., Kulshrestha, S., Raikhy, G., Hallan, V.,
   Ram, R. and Zaidi, A. A. (2004). Occurrence of Cucumber mosaic virus in
      Gerbera jamesonii from India. Plant Disease 88(10): 1161. (Impact Factor:
      1.790).

  22.Kulshrestha, S., Hallan, V., Raikhy, G., Ram, R. and Zaidi, A. A. (2004).
     Strawberry latent ringspot virus (SLRSV) Infecting Roses in India. Plant
     Disease 88(1): 86. (Impact Factor: 1.790).

  23.Raikhy, G., Hallan, V., Kulshrestha, S., Sharma, M. L., Ram, R. and Zaidi, A. A.
     (2003). Molecular Characterization of a Carnation etched ring virus Isolate from
     India. Acta Virologica 47 (2): 105-111. (Impact Factor: 0.560).

  24.Raikhy, G., Hallan, V., Kulshrestha, S., Sharma, M. L., Ram, R. and Zaidi, A. A.
     (2003). First report of Carnation necrotic fleck virus (CNFV) infecting
     Carnations in India. Plant Pathology 52: 801. (Impact Factor: 2.012).

  25.Ram, R., Joshi, A., Verma, N., Kulshrestha, S., Raikhy, G., Hallan, V. and Zaidi,
     A. A. (2003). First report of Dasheen mosaic virus infecting four ornamental
     aroids in India. Plant Pathology 52: 411. (Impact Factor: 2.012).

PAPER UNDER PREPARATION

  1. Kulshrestha, S., Patrick, D. P., Venugopal, S. C., Chanda, B., Kachroo, A.,
     Kachroo, P. and Vaillancourt, L. NAD-dependent glycerol-3-phosphate
     dehydrogenase is required for pathogenicity of the hemibiotrophic pathogen
     Colletotrichum higginsianum to Arabidopsis thaliana. In: Molecular Plant
     Microbe Interaction (Impact Factor: 4.275).

PATENTS FILED

  1. Kulshrestha, S., Hallan, V., Raikhy, G. and Zaidi, A. A. Development of
     diagnostic kit against the recombinant Coat Protein (CP) of Prunus necrotic
     ringspot virus (Number: 0069 NF 2005/ IN / 25.7.05, US 20070026390).

  2. Raikhy, G., Hallan, V., Kulshrestha, S. and Zaidi, A. A. Diagnostic kit for the
     detection of Carnation mottle virus (Number: 0068 NF 2005 / IN / 25.7.05,
     PCT/IB2006/002019).

  3. Raikhy, G., Hallan, V., Kulshrestha, S. and Zaidi, A. A. Development of ELISA
     based diagnostic kit against the recombinant Coat Protein (CP) of Indian isolate of
     Carnation etched ring virus (Number: 00185 NF 2005).

PUBLISHED ABSTRACTS OF PAPERS/POSTERS PRESENTED IN VARIOUS
CONFERENCES:

  1. Kulshrestha, S., Kumar, V., Hallan, V., Ram, R., Garg, I. D. and Zaidi, A. A.
     Detection and characterization of Indian strain of Bean yellow mosaic virus
     (BYMV) infecting Iris. In xxvi Annual Conference on Electron Microscopy and
     Allied Fields, from 16-18 April 2003 at CPRI, Shimla, India.

  2. Kulshrestha, S., Kumar, A., Hallan, V., Ram, R., Garg, I. D. and Zaidi, A. A.
     Characterization of Indian isolate of Iris severe mosaic virus causing mosaic
     disease on Iris. In xxvi Annual Conference on Electron Microscopy and Allied
     Fields, from 16-18 April 2003 at CPRI, Shimla, India.

  3. Kulshrestha, S., Hallan, V., Raikhy, G., Ram, R., Garg, I. D. and Zaidi, A. A.
     Incidence of Bean yellow mosaic virus on Iris. In the 11th International
     Symposium on Virus Diseases of Ornamental Plants, from 09-14 March, 2004 at
     National Museum of Natural Science, Taichung, Taiwan, R. O. C.

  4. Hallan, V., Singh, H.P., Sharma, M. L. Kulshrestha, S., Raikhy, G., Ram, R.,
     Garg, I.D. and Zaidi, A. A. Detection and molecular characterization of Indian
     strain of Carnation mottle virus (CarMV) infecting carnation. In xxvi Annual
     Conference on Electron Microscopy and Allied Fields, from 16-18 April 2003 at
     CPRI, Shimla, India.

  5. Raikhy, G., Hallan, V., Kulshrestha, S., Ram, R. and Zaidi, A. A. Detection of
     Carnation ringspot and Carnation vein mottle virus(es) in Carnation cultivars in
     India. In the 11th International Symposium on Virus Diseases of Ornamental
     Plants, from 09-14 March, 2004 at National Museum of Natural Science,
     Taichung, Taiwan, R. O. C.


SEQUENCES OF DIFFERENT VIRUS GENES SUBMITTED TO EMBL
DATABASE:  30


SEQUENCES OF DIFFERENT PRIMERS DESIGNED AND SUBMITTED TO
EMBL DATABASE:      22

				
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