Faecal Calprotectin home test in Ulcerative Colitis by pcu59739

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									Faecal Calprotectin home test in Ulcerative Colitis
                 - is it possible?
                      Margarita Elkjaer1, Johan Burisch1, Arne Røseth2, Vibeke Voxen Hansen1, Birgit Deibjerg Kristensen1,
                                                          3                         4               1
                                            Dag Bremnes , Jens-Kristian Slott Jensen , Pia Munkholm
                                                1
                                                Herlev University Hospital, Copenhagen, Denmark
                                                     2
                                                       Aker University hospital, Oslo Norway
                                                           3
                                                             Skannex AS, Oslo, Norway
                                                              4
                                                                Slott Stat, Bagsværd




1                                                   2                                                                     3




Background                                              Results
• Enzyme-linked immunosorbent assay                 Figure 1. Comparison of RTSCAN vs. ELISA                              • 175 faecal samples have been collected
  (ELISA) is a quantitative method & the            of measuring faecal Calprotein (mg/kg)                                  from ulcerative colitis (UC) patients in-
  “Gold standard” for faecal Calprotectin                                                                                   volved in a randomised control trial “Con-
  (FC) analysis                                                                                                             stant-care”
                                                              8000
                                                              7000
• A new quantitative method, the ”Rapid                       6000
                                                                                                                          • ELISA vs. RTSCAN mean difference of
  Test” (RT), has been developed to be                        5000                                                          5.1 mg/kg (95% CI; ÷16.5-26.6) fig 1
  carried out by the lab technician, in order
                                                     ELISA




                                                              4000
  to have the results the same day as the                     3000                                                        • ELISA vs. HTPHOTO mean difference of
  faeces sample is received.                                  2000                                                          26.7 mg/kg (95% CI; 1.3-52.2) fig 2
                                                              1000
                                                                 0
• RT has further been developed into a                               0   1000   2000   3000   4000   5000   6000   7000
                                                                                                                          • RTSCAN vs. HTPHOTO mean difference
  “Home Test” (HT) to be carried out by the                                              RTSCAN                             of 21.7 mg/kg (95% CI; 11.5-32.0) fig 3
  patients themselves
                                                                                                                          • The coefficient of variation (CV %) of ELI-
                                                                                                                            SA was in median 2.8%
                                                    Figure 2. Comparison of HTPHOTO vs. ELISA                               (range, 0-21.6%) vs. HTPHOTO 6.2%
                                                    of measuring faecal Calprotein (mg/kg)                                  (range, 0-41.6%), p-value <0.01
Aim
• To compare the ELISA with the RT´s for                      8000
  FC measurement                                              7000

  - read by a PC-scanner system                               6000
                                                              5000
                                                                                                                          Conclusion
  - using an ordinary cell phone system
                                                     ELISA




                                                              4000
    with special software built in (DB)                       3000
                                                                                                                          A positive correlation between ELISA vs.
                                                              2000
                                                                                                                          Home Test was revealed with the CV is
                                                              1000                                                        being less than 10%.
                                                                0
                                                                     0   1000   2000   3000   4000   5000   6000   7000   In future aspects the Home Test can be im-
                                                                                        HTPHOTO
                                                                                                                          plemented in UC patients as part of disease
Methods                                                                                                                   self-management.

• After dilution one drop of faecal extract         Figure 3. Comparison of HTPHOTO vs. RTSCAN
  was applied onto the RT- device                   of measuring faecal Calprotein (mg/kg)
• The colour intensity of the test line was
  read after 10 min incubation time                           7000
  - using a lap-top linked with an ordinary                   6000
    table top scanner (photo 1 and 2)                         5000
  - using a cell phone by taking & sending                    4000
                                                     RTSCAN




    a picture to a server in Oslo by using                    3000
    Mobile Internet (photo 3)                                 2000

                                                              1000
In this study the RT including HT was carried
                                                                 0
out by our lab technicians.                                          0   1000   2000   3000   4000   5000   6000   7000
                                                                                         HTPHOTO
                                                                                                                                                                          Grafik & TV • grafik@heh.regionh.dk • 14021

								
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