Biology 5610 Spring 2009 Mouse Dissection This laboratory exercise provides you with an opportunity to dissect a recently eutha- nized and unpreserved mouse (representative mammal). The chemicals used for pre- serving animal tissues (e.g., formaldehyde) are odiferous cross-linking agents that chemically alter the physical properties of the animal tissues. Thus, the advantage of using a fresh-- as opposed to preserved--mouse is that you will be able to sense the normal colors, odors and textures of the tissues and organs. However, you should bear in mind that the tissues will be oxygen-deprived and hence may have a darker color than in the living mouse. Nonetheless, this dissection exercise should give you a better idea about the real properties of living animal tissues. Precautions: You should make every attempt to avoid contact with mouse body ﬂuids. Wear gloves, and eye protection for this dissection. No eating or drinking in lab. DO NOT touch your face or anything that will come into contact with your nose or mouth while you are wearing the gloves that you used to handle the mouse. Wash your hands thoroughly with anti-bacterial soap before exiting the lab. 1) You will use an online guide to mouse dissection, provided for you. 2) Your lab report will NOT use the standard format. Instead, you will make a detailed description of the appearance of each tissue and organ as follows: A) Identity of organ B) Location C) Shape D) Size (relative to other organs) E) Color F) Texture G) Consistency (i.e. ﬁrm or soft) H) Major Function(s) (CITE this information) Biology 5610 Spring 2009 Organs to be identiﬁed: 1) Axillary lymph nodes 2) Inguinal lymph nodes 3) Salivary gland 4) Rib Cage 5) Diaphragm 6) Intestines a) small intestine b) large intestine 7) Stomach 8) Liver 9) Urinary Bladder 10) Pancreas 11) Spleen 12) Kidneys 13) Adrenals 14) Heart 15) Thymus 16) Lungs 3) State the phylogenetic classiﬁcation of the mouse, including Kingdom, Phylum, Class, Order, Family, Genus and Species. Include literature cited where appropriate (which means that every question, including the tissue/organ spreadsheet, needs to be cited).
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