Nutrient Sampling Procedure Nutrient sampling is to be done weekly in conjunction with Hydrolab physical water quality data collection. Equipment needed: Peristaltic field pump Deep cycle marine battery Tygon (or Masterflex) line Plastic tubing for deep well Nalgene collection bottles Hydrolab Beaker DI wash bottle Field bag with tools for removing well covers datasheet Purge each monitoring well into beaker until temperature measurement on the Hydrolab equilibrates. Once temperature has stabilized record the Hydrolab readings on the data sheet. Remove the Hydrolab from the beaker and begin pumping sample into Nalgene bottle. Fill to overflowing to displace any oxygen. Label sample bottle. Rinse Hydrolab probes When you return to the lab if you are not able to process your samples immediately be sure to put them in the refrigerator in SL118.