GENE TRANSFER AND GENETIC ENGINEERING by fop21123

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									GENE TRANSFER AND
GENETIC ENGINEERING
      CHAPTER 8




                      1
Gene Transfer

       Vertical



       Lateral (horizontal)




                               2
Bacterial Gene Transfer and
Recombinant DNA Technology
      Transformation
       – Griffith’s
         experiment




                              3
Transformation
       Mechanism




       Significance




                       4
Transduction
      Mechanism




      Significance




                      5
Conjugation
      Mechanism




      Significance




                      6
Plasmids
      F plasmid

      Resistance plasmids

      Virulence plasmids




                             7
Plasmids (cont.)
      Tumor-inducing plasmids




      Plasmids conferring novel catabolism




                                              8
Genetic Engineering
      Genetic fusions




                         9
Genetic Engineering
       Protoplast fusion




                            10
Genetic Engineering (cont.)
      Gene
       Amplification




      Recombinant
       DNA cloning



                              11
Applications

       Medical

       Industrial

       Agricultural

       Hybridomas

                       12
Risks and Benefits
      ELSI



      Concerns




                     13
Recombinant DNA technology




                             14

								
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