Curriculum Vitae Kerry Edythe Preston Georgia Institute of Technology KerryHolland@gatech.edu 311 Ferst Dr, ES&T Room 3368 Work (404) 385-4573 Atlanta, GA 30332 Home (678) 984-8131 Education PhD Environmental Engineering (in progress), Georgia Institute of Technology, Atlanta, Ga, 8/2005-present. MS Environmental Engineering, Georgia Institute of Technology, Atlanta, Ga, 5/2005. BS Chemistry, Emory University, Atlanta, Ga, 5/1998. Professional Affililations; Awards American Society for Microbiology; 2005-present American Society of Mass Spectrometry; 2005-present Association of Professional Women; 2005-present Outstanding Poster Presentation Award; Association of Biomolecular Resources Facilities; 2/2005 Member, Phi Beta Kappa, inducted 1997. Oxford College of Emory University’s Excellence in Organic Chemistry Award 1996 Oxford College of Emory University’s Excellence in General Chemistry Award 1995 Work Experience 7/07-present Graduate Research Assistant Georgia Institute of Technology Department of Civil and Environmental Engineering, Advisor: Dr. Frank Löffler Collaborate with researchers at the CDC and GIT to develop mass-spectrometry based techniques for the detection and characterization of environmentally significant microorganisms which (a) detoxify or immobilize common brownfield contaminants such as radionuclides, metals, and chlorinated phenols, and (b) mediate the flux of the greenhouse gas nitrous oxide. Develop and apply proteomic technologies toward the identification and quantification of protein biomarkers specific to strains of the metabolically versatile bacterium Anaeromyxobacter dehalogenans in pure cultures, consortia, and environmental samples. Perform laboratory experiments to elucidate patterns of differential gene expression by A.dehalogenans under various environmentally significant growth conditions. Utilize nucleic-acid-based techniques such as real-time quantitative polymerase chain reaction (qPCR), using primers designed in the Löffler lab, to detect and quantify microbial targets in environmental samples. Integrate transcriptomic data from interrogation of whole-genome microarrays with proteomics data from liquid chromatography/mass-spectrometry and 2-dimensional gel electrophoresis experiments as complementary and cross-validation techniques to study contaminant removal by A.dehalogenans strains. Maintain anaerobic lineages of A.dehalogenans strains in either pure culture or consortia using sterile technique. 6/02 – 7/07 Research Chemist Centers for Disease Control and Prevention (CDC) National Center for Environmental Health (NCEH) Emergency Response and Air Toxicant Branch (ERAT) Supervisor: Dr. John Barr Developed and validated mass-spectrometry based analytical methods for identification and quantification of metabolites and adducts of chemical and biological warfare agents, including nerve agents, phosgene, sulfur mustards, and ricin in clinical samples Maintain validated methods for readiness in the event of a chemical or biological terrorism attack or other human exposure to warfare agents Operated and maintained tandem GC- or LC-mass spectrometers including the Finnigan TSQ 7000 triple-quad GC/MS/MS and MAT 900 XL Trap high resolution magnetic sector GC/MS, and Applied Biosystems/MDS SCIEX API 4000 LC/MS/MS triple quadrupole and QTrap systems with accompanying Excalibur, Analyst, or Unix-based software Operated and maintained Hewlett-Packard 6890 gas chromatographs and Agilent 1100 and 1200 series liquid chromatographs with various autosamplers Managed data using MS Access-based database created for tracking of ERAT validation and proficiency data, MS Excel, Excalibur quantification software, and laboratory response network Wrote Standard Operating Procedures and/or CLIA manuals for nerve agent adducts and metabolites Performed quarterly proficiency testing challenges to maintain readiness for analysis of nerve agent metabolites Served as primary emergency responder for nerve agent adduct analyses and secondary responder for nerve agent metabolite analyses 10/01- 7/02 Oak Ridge Institute for Science and Education (ORISE) Research Fellow Centers for Disease Control and Prevention, Organic Analytical Toxicology Branch [CDC/NCEH/DLS/OAT] Supervisors: Dr. Andreas Sjodin and Dr. Courtney Sandau Collaborated in development of semi-automated rapid methods for biomonitoring of persistent organic pollutants in human serum samples Operated, maintained, and calibrated Agilent HPLC with accompanying ChemStation software Operated Agilent GC/MSD in both electron impact and negative chemical ionization modes with Enhanced ChemStation software Operated and maintained Zymark Rapidtrace automated solid phase extraction systems and operating software; includes extensive troubleshooting Wrote Standard Operating Procedure (SOP) and appendices for a newly validated method for analyzing polychlorinated biphenyls and pesticides Acquired extensive working knowledge of chemistry of persistent organic pollutants and broad experience with various separation technologies such as solid phase extraction and gas, liquid, and gel permeation chromatography Prepared and processed samples which included spiking of standards and handling human serum Managed and tracked laboratory samples by organizing sample sets, documenting sample processing, and keeping official notebooks for experiments and studies 8/00 – 9/01 High School Chemistry and Physics Teacher South Gwinnett High School Science Department Principal: Bobby Crowson Taught four sections of College Prep (CP) Physics and one of CP Chemistry to sophomore, junior and senior high school students Developed laboratory experiments and demonstrations Served as coach and faculty sponsor for the Dance Team: coordinated regular practice sessions, arranged registration and travel for competitions, and managed finances and choreographer contracting 5/98 – 10/99 Production Supervisor Cleveland Technical Centers (CTC) Analytical Services Supervisor: Michael Costello Supervised approximately twenty technicians for the analytical chemistry laboratory of an ISO 9002-certified oil analysis provider Ensured that sample processing deadlines were met for an average of 1500 samples per day and that efficiency parameters were continually improved Maintained OASIS database to manage multi-instrument data Responsible for upkeep of scientific equipment such as ICP, FT-IR, Rotary Disc Electrode, Viscometers, and TAN/TBN counters, and for implementation of new instruments Wrote production reports, tracked inventory and supplies, and handled personnel problems Wrote Standard Operating Procedures (SOPs) for ICP spectrometers, FT-IRs and viscometers Orchestrated all technician training Upgraded training program as a special project, including rewriting training manuals and examinations 5/97 – 5/98 General Electric Research Fellow Emory University Organic Chemistry Department Faculty Research Advisor: Dr. William Crowe Received GE grant to pursue the optimization of the synthesis of a human hormone called Corey Lactone Devised and tested synthetic approaches and presented data and results to colleagues Operated Nuclear Magnetic Resonance (NMR) spectrometer to characterize compounds Routinely utilized air-sensitive technique 5/96-8/96 National Science Foundation/Research Experience for Undergraduates (NSF/REU) Research Intern Columbia University Organic Chemistry Department Faculty Research Advisor: Dr. Thomas Katz Researched the novel, asymmetric synthesis of a biologically active amine Attempted the synthesis of helicenes, or spiral-shaped molecules with important electromagnetic properties Operated analytical equipment including HPLC and NMR Publications K.E. Holland, H. Moura, A. Woolfitt, Q. Wu, S. Thomas, Y. Sung, J. R. Barr, F.E. Loeffler. Specificity and Variation of Intact-Cell MALDI-TOF MS Profiles of Anaeromyxobacter dehalogenans. In process. K.E. Holland, M.I. Solano, R. Johnson, V. Maggio, J. R. Barr. Modifications to the Organophosphorus Nerve Agent-Butyrylcholinesterase Adduct Refluoridation Method for Retrospective Analysis of Nerve Agent Exposures. J. Anal. Toxicol., 2007, in press. K.E. Preston, M.I. Solano, V.L. Maggio, J.R. Barr, C.E.A.M. Degenhardt, K. Pleijsier, M.J. van der Schans, J.P. Langenberg, Improvements of the Fluoride Reactivation Method for the Verification of Nerve Agent Exposure. J. Anal. Toxicol., 2004, 28(5), 364-371. R.C. Johnson, S.W. Lemire, A.R. Woolfitt, M. Ospina, K.E. Preston, C.T. Olson, J.R. Barr. Quantification of ricinine in rat and human urine: a biomarker for ricin exposure. J. Anal. Toxicol., 2005, 29(3), 149-55. A.E. Boyer, D. Ash, D.B. Barr, C.L. Young, W.J. Driskell, R.D. Whitehead, Jr., M. Ospina, K.E. Preston, A.R.Woolfitt, R.A. Martinez, L.A. Silks, J. R. Barr. Quantitation of the sulfur mustard metabolites 1,1-Sulfonylbis[2-(methylthio)ethane] and thiodiglycol in urine using isotope dilution gas chromatography-tandem mass spectrometry. J. Anal. Toxicol., 2004, 28(5), 327-332. C.D. Sandau, A. Sjodin, M.D. Davis, J.R. Barr, V.L. Maggio, A.L. Waterman, K.E. Preston, J.L. Preau, Jr., D.B. Barr, L.L. Needham, D.G. Patterson, Jr. Comprehensive solid-phase extraction method for persistent organic pollutants. Validation and application to the analysis of persistent chlorinated pesticides. Anal Chem. 2003, 75(1),71-7. Presentations K.E. Preston, F.E. Loeffler, A.Woolfitt, H. Moura, S.H. Thomas, Q.Wu, Y. Sung, J. R. Barr, Detection of substrate-specific markers for metabolically versatile bacteria by whole-cell MALDI-TOF MS presented to the American Chemical Society National Meeting, San Francisco CA, September 10-14, 2006. K. E. Preston, M. I. Solano, A. Pavlopoulos, D.Mawhinney, J. R. Barr, D. Ashley, VX Exposure Analysis presented to the Army, Aberdeen Proving Grounds, Edgewood MD, June 20, 2006. K. E. Preston, J. R. Barr, A. Woolfitt, H. Moura, S.H. Thomas, Q. Wu, Y. Sung, F. E. Loeffler, Characterization of Anaeromyxobacter dehalogenans and Related Metal-Reducing Bacteria by MALDI-TOF MS presented to the DOE Environmental Remediation Sciences Division Meeting, Warrenton VA, April 3-5, 2006. K.E. Preston, M.I. Solano, J. Wooten, R. Johnson, J.R. Barr, Analysis of Nerve Agent Adducts for Retrospective Exposure Assessment presented to the CDC Laboratory Response Network, Atlanta, GA, November 15, 2005. K.E. Preston, H. Moura, A. Woolfitt, B. Amos, Y. Sung, S. Henry, J. R. Barr, F. E. Loeffler, MALDI-TOF MS Profiling of Metal-Reducing Bacteria Including Anaeromyxobacter, Geobacter, and Desulfuromonas Species presented to the International Society for Subsurface Microbiology (ISSM), Jackson Hole, WY, August 17, 2005. K.E. Preston, H. Moura, A. Woolfitt, B. Amos, Y. Sung, S. Henry, J. R. Barr, F. E. Loeffler, MALDI-TOF MS Profiling of Environmentally Relevant Bacteria Including Anaeromyxobacter, Geobacter, and Desulfuromonas Species presented to the American Society for Microbiology (ASM), Atlanta, Ga, June 8, 2005. K.E. Preston, M.I. Solano, A.J. Pavlopoulos, J.R. Barr, Improved Methods for Retrospective Analysis of Nerve Agent Exposure presented to the Pittsburgh Conference, Orlando, FL, March 2, 2005. K.E. Preston, F.E. Loeffler, H. Moura, A. Woolfitt, B. Amos, Y. Sung, S. Henry, J. R. Barr, Detection of Anaeromyxobacter dehalogenans Biomarkers by MALDI-TOF-MS Analysis of Whole Cells presented to the Association of Biomolecular Resourse Facilities (ABRF) Savannah, GA, February 5, 2005.
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