Publications by tyndale

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									                                    Curriculum Vitae
                                   Kerry Edythe Preston

 Georgia Institute of Technology                                      KerryHolland@gatech.edu
 311 Ferst Dr, ES&T Room 3368                                             Work (404) 385-4573
 Atlanta, GA 30332                                                        Home (678) 984-8131


Education
      PhD Environmental Engineering (in progress), Georgia Institute of Technology, Atlanta,
       Ga, 8/2005-present.
      MS Environmental Engineering, Georgia Institute of Technology, Atlanta, Ga, 5/2005.
      BS Chemistry, Emory University, Atlanta, Ga, 5/1998.

Professional Affililations; Awards
      American Society for Microbiology; 2005-present
      American Society of Mass Spectrometry; 2005-present
      Association of Professional Women; 2005-present
      Outstanding Poster Presentation Award; Association of Biomolecular Resources
       Facilities; 2/2005
      Member, Phi Beta Kappa, inducted 1997.
      Oxford College of Emory University’s Excellence in Organic Chemistry Award 1996
      Oxford College of Emory University’s Excellence in General Chemistry Award 1995

Work Experience
7/07-present             Graduate Research Assistant
                         Georgia Institute of Technology
                         Department of Civil and Environmental Engineering,
                         Advisor: Dr. Frank Löffler
      Collaborate with researchers at the CDC and GIT to develop mass-spectrometry based
       techniques for the detection and characterization of environmentally significant
       microorganisms which (a) detoxify or immobilize common brownfield contaminants
       such as radionuclides, metals, and chlorinated phenols, and (b) mediate the flux of the
       greenhouse gas nitrous oxide.
      Develop and apply proteomic technologies toward the identification and quantification of
       protein biomarkers specific to strains of the metabolically versatile bacterium
       Anaeromyxobacter dehalogenans in pure cultures, consortia, and environmental samples.
      Perform laboratory experiments to elucidate patterns of differential gene expression by
       A.dehalogenans under various environmentally significant growth conditions.
      Utilize nucleic-acid-based techniques such as real-time quantitative polymerase chain
       reaction (qPCR), using primers designed in the Löffler lab, to detect and quantify
       microbial targets in environmental samples.
      Integrate transcriptomic data from interrogation of whole-genome microarrays with
       proteomics data from liquid chromatography/mass-spectrometry and 2-dimensional gel
       electrophoresis experiments as complementary and cross-validation techniques to study
       contaminant removal by A.dehalogenans strains.
      Maintain anaerobic lineages of A.dehalogenans strains in either pure culture or consortia
       using sterile technique.
6/02 – 7/07             Research Chemist
                        Centers for Disease Control and Prevention (CDC)
                        National Center for Environmental Health (NCEH)
                        Emergency Response and Air Toxicant Branch (ERAT)
                        Supervisor: Dr. John Barr
      Developed and validated mass-spectrometry based analytical methods for identification
       and quantification of metabolites and adducts of chemical and biological warfare agents,
       including nerve agents, phosgene, sulfur mustards, and ricin in clinical samples
      Maintain validated methods for readiness in the event of a chemical or biological
       terrorism attack or other human exposure to warfare agents
      Operated and maintained tandem GC- or LC-mass spectrometers including the Finnigan
       TSQ 7000 triple-quad GC/MS/MS and MAT 900 XL Trap high resolution magnetic
       sector GC/MS, and Applied Biosystems/MDS SCIEX API 4000 LC/MS/MS triple
       quadrupole and QTrap systems with accompanying Excalibur, Analyst, or Unix-based
       software
      Operated and maintained Hewlett-Packard 6890 gas chromatographs and Agilent 1100
       and 1200 series liquid chromatographs with various autosamplers
      Managed data using MS Access-based database created for tracking of ERAT validation
       and proficiency data, MS Excel, Excalibur quantification software, and laboratory
       response network
      Wrote Standard Operating Procedures and/or CLIA manuals for nerve agent adducts and
       metabolites
      Performed quarterly proficiency testing challenges to maintain readiness for analysis of
       nerve agent metabolites
      Served as primary emergency responder for nerve agent adduct analyses and secondary
       responder for nerve agent metabolite analyses

10/01- 7/02     Oak Ridge Institute for Science and Education (ORISE) Research Fellow
                Centers for Disease Control and Prevention,
                Organic Analytical Toxicology Branch [CDC/NCEH/DLS/OAT]
                Supervisors: Dr. Andreas Sjodin and Dr. Courtney Sandau
      Collaborated in development of semi-automated rapid methods for biomonitoring of
       persistent organic pollutants in human serum samples
      Operated, maintained, and calibrated Agilent HPLC with accompanying ChemStation
       software
      Operated Agilent GC/MSD in both electron impact and negative chemical ionization
       modes with Enhanced ChemStation software
      Operated and maintained Zymark Rapidtrace automated solid phase extraction systems
       and operating software; includes extensive troubleshooting
      Wrote Standard Operating Procedure (SOP) and appendices for a newly validated method
       for analyzing polychlorinated biphenyls and pesticides
      Acquired extensive working knowledge of chemistry of persistent organic pollutants and
       broad experience with various separation technologies such as solid phase extraction and
       gas, liquid, and gel permeation chromatography
      Prepared and processed samples which included spiking of standards and handling
       human serum
      Managed and tracked laboratory samples by organizing sample sets, documenting sample
       processing, and keeping official notebooks for experiments and studies
8/00 – 9/01    High School Chemistry and Physics Teacher
               South Gwinnett High School
               Science Department
               Principal: Bobby Crowson
      Taught four sections of College Prep (CP) Physics and one of CP Chemistry to
       sophomore, junior and senior high school students
      Developed laboratory experiments and demonstrations
      Served as coach and faculty sponsor for the Dance Team: coordinated regular practice
       sessions, arranged registration and travel for competitions, and managed finances and
       choreographer contracting

5/98 – 10/99   Production Supervisor
               Cleveland Technical Centers (CTC) Analytical Services
               Supervisor: Michael Costello
      Supervised approximately twenty technicians for the analytical chemistry laboratory of
       an ISO 9002-certified oil analysis provider
      Ensured that sample processing deadlines were met for an average of 1500 samples per
       day and that efficiency parameters were continually improved
      Maintained OASIS database to manage multi-instrument data
      Responsible for upkeep of scientific equipment such as ICP, FT-IR, Rotary Disc
       Electrode, Viscometers, and TAN/TBN counters, and for implementation of new
       instruments
      Wrote production reports, tracked inventory and supplies, and handled personnel
       problems
      Wrote Standard Operating Procedures (SOPs) for ICP spectrometers, FT-IRs and
       viscometers
      Orchestrated all technician training
      Upgraded training program as a special project, including rewriting training manuals and
       examinations

5/97 – 5/98   General Electric Research Fellow
              Emory University Organic Chemistry Department
              Faculty Research Advisor: Dr. William Crowe
      Received GE grant to pursue the optimization of the synthesis of a human hormone called
       Corey Lactone
      Devised and tested synthetic approaches and presented data and results to colleagues
      Operated Nuclear Magnetic Resonance (NMR) spectrometer to characterize compounds
      Routinely utilized air-sensitive technique

5/96-8/96       National Science Foundation/Research Experience for Undergraduates
               (NSF/REU) Research Intern
               Columbia University Organic Chemistry Department
               Faculty Research Advisor: Dr. Thomas Katz
      Researched the novel, asymmetric synthesis of a biologically active amine
      Attempted the synthesis of helicenes, or spiral-shaped molecules with important
       electromagnetic properties
      Operated analytical equipment including HPLC and NMR
Publications

K.E. Holland, H. Moura, A. Woolfitt, Q. Wu, S. Thomas, Y. Sung, J. R. Barr, F.E. Loeffler.
Specificity and Variation of Intact-Cell MALDI-TOF MS Profiles of Anaeromyxobacter
dehalogenans. In process.

K.E. Holland, M.I. Solano, R. Johnson, V. Maggio, J. R. Barr. Modifications to the
Organophosphorus Nerve Agent-Butyrylcholinesterase Adduct Refluoridation Method for
Retrospective Analysis of Nerve Agent Exposures. J. Anal. Toxicol., 2007, in press.

K.E. Preston, M.I. Solano, V.L. Maggio, J.R. Barr, C.E.A.M. Degenhardt, K. Pleijsier, M.J. van
der Schans, J.P. Langenberg, Improvements of the Fluoride Reactivation Method for the
Verification of Nerve Agent Exposure. J. Anal. Toxicol., 2004, 28(5), 364-371.

R.C. Johnson, S.W. Lemire, A.R. Woolfitt, M. Ospina, K.E. Preston, C.T. Olson, J.R. Barr.
Quantification of ricinine in rat and human urine: a biomarker for ricin exposure. J. Anal.
Toxicol., 2005, 29(3), 149-55.

A.E. Boyer, D. Ash, D.B. Barr, C.L. Young, W.J. Driskell, R.D. Whitehead, Jr., M. Ospina, K.E.
Preston, A.R.Woolfitt, R.A. Martinez, L.A. Silks, J. R. Barr. Quantitation of the sulfur mustard
metabolites 1,1-Sulfonylbis[2-(methylthio)ethane] and thiodiglycol in urine using isotope dilution
gas chromatography-tandem mass spectrometry. J. Anal. Toxicol., 2004, 28(5), 327-332.

C.D. Sandau, A. Sjodin, M.D. Davis, J.R. Barr, V.L. Maggio, A.L. Waterman, K.E. Preston, J.L.
Preau, Jr., D.B. Barr, L.L. Needham, D.G. Patterson, Jr. Comprehensive solid-phase extraction
method for persistent organic pollutants. Validation and application to the analysis of persistent
chlorinated pesticides. Anal Chem. 2003, 75(1),71-7.


Presentations

K.E. Preston, F.E. Loeffler, A.Woolfitt, H. Moura, S.H. Thomas, Q.Wu, Y. Sung, J. R. Barr,
Detection of substrate-specific markers for metabolically versatile bacteria by whole-cell
MALDI-TOF MS presented to the American Chemical Society National Meeting, San Francisco
CA, September 10-14, 2006.

K. E. Preston, M. I. Solano, A. Pavlopoulos, D.Mawhinney, J. R. Barr, D. Ashley, VX Exposure
Analysis presented to the Army, Aberdeen Proving Grounds, Edgewood MD, June 20, 2006.

K. E. Preston, J. R. Barr, A. Woolfitt, H. Moura, S.H. Thomas, Q. Wu, Y. Sung, F. E. Loeffler,
Characterization of Anaeromyxobacter dehalogenans and Related Metal-Reducing Bacteria by
MALDI-TOF MS presented to the DOE Environmental Remediation Sciences Division Meeting,
Warrenton VA, April 3-5, 2006.

K.E. Preston, M.I. Solano, J. Wooten, R. Johnson, J.R. Barr, Analysis of Nerve Agent Adducts
for Retrospective Exposure Assessment presented to the CDC Laboratory Response Network,
Atlanta, GA, November 15, 2005.

K.E. Preston, H. Moura, A. Woolfitt, B. Amos, Y. Sung, S. Henry, J. R. Barr, F. E. Loeffler,
MALDI-TOF MS Profiling of Metal-Reducing Bacteria Including Anaeromyxobacter,
Geobacter, and Desulfuromonas Species presented to the International Society for Subsurface
Microbiology (ISSM), Jackson Hole, WY, August 17, 2005.

K.E. Preston, H. Moura, A. Woolfitt, B. Amos, Y. Sung, S. Henry, J. R. Barr, F. E. Loeffler,
MALDI-TOF MS Profiling of Environmentally Relevant Bacteria Including Anaeromyxobacter,
Geobacter, and Desulfuromonas Species presented to the American Society for Microbiology
(ASM), Atlanta, Ga, June 8, 2005.

K.E. Preston, M.I. Solano, A.J. Pavlopoulos, J.R. Barr, Improved Methods for Retrospective
Analysis of Nerve Agent Exposure presented to the Pittsburgh Conference, Orlando, FL, March
2, 2005.

K.E. Preston, F.E. Loeffler, H. Moura, A. Woolfitt, B. Amos, Y. Sung, S. Henry, J. R. Barr,
Detection of Anaeromyxobacter dehalogenans Biomarkers by MALDI-TOF-MS Analysis of
Whole Cells presented to the Association of Biomolecular Resourse Facilities (ABRF) Savannah,
GA, February 5, 2005.

								
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