2009 Revised Plant Pathology Core Series Fall Quarter (Year 1) PLP 120- required of all unless the equivalent has been taken elsewhere Winter Quarter: 2 modules, each 5 weeks PLP 202A. Advanced Plant Pathology: Introductory module. 2 units, Three 1 hour lectures, one 1 hour discussion per week Topics: 1. Symbiotic relationships in nature: organelles; legume/Rhizobium nodules, epiphytic and endophytic microbes 2. The evolutionary significance of parasitism and the Red Queen hypothesis 3. The ecological roles of parasitism in native plant communities: the Janzen Connell Hypothesis and frequency dependent selection. 4. Diseases in native plant communities: rubber leaf blight, anther smut and flax rust 5. Exotic diseases in native ecosystems: chestnut blight and others 6. The transition to agriculture: powdery mildew of barley in native and managed plant communities 7. Powdery mildew of barley (continued) 8. Pathways to pathogenesis: how endophytes become latent pathogens 9. Genetics of pathogen populations breeding biology and other factors that influence changes in virulence. 10. Population genetics of host-pathogen interactions: rust diseases of wheat 11. Ecology of soil microbes in agroecosystems 12. The influence of plant roots and agricultural practices on the activity of soilborne pathogens. 13. The phyllosphere as a theatre for microbial activity – the effects of host condition, environment and competition on potential pathogens. 14. Disease cycles – using our understanding of pathogen biology to develop management strategies 15. Future directions PLP 202B. Advanced Plant Pathology: Bacteriology. 2 units, Three 1 hour lectures, one 1 hour discussion per week Topics: 1. Course introduction and requirements. Overview of the major groups of phytopathogenic prokaryotes and the diseases they cause. 2. Taxonomy and systematics of phytopathogenic prokaryotes. 3. Taxonomy and systematics of phytopathogenic prokaryotes (cont.) 4. Leaf spot diseases caused by Pseudomonas and Xanthomonas characteristics of the diseases and the pathogens 5. Leaf spot diseases: the pathovar system, pathogen detection and disease management 6. Wilt and rot diseases caused by Pseudomonas, Xanthomonas and Erwinia: characteristics of the diseases and the pathogens 7. Wilt diseases: mechanisms of pathogenicity. New approaches for disease control 8. Bacterial biofilm and quorum sensing 9. Biology, epidemiology and control of Agrobacterium spp. 10. Molecular basis of crown gall pathogenesis 11. Wall-less, fastidious prokaryotes: Candidatus Phytoplasma and Spiroplasma biological properties, epidemiology and control 12. Walled, fastidious prokaryotes (Xylella, Clavibacter and Candidatus Liberobacter), emerging diseases, biology, epidemiology and control 13. Overview of genomic analyses of plant pathogenic bacteria 14. Prokaryote genes involved in plant pathogenesis; avr, hrp, etc. 15. Prokaryote genes involved in plant pathogenesis Spring Quarter: 2 modules, each 5 weeks PLP 202C. Advanced Plant Pathology: Virology. 2 units, Three 1 hour lectures, one 1 hour discussion per week Topics: 1. Some history, the virus concept, what viruses do. 2. Some virus taxonomy-classification. 3. TMV 4. TMV/BMV 5. BMV/CMV, Sindbis lineage 6. Potyvirus genome organization Picornavirus lineage 7. Potyvirus aphid transmission and biology 8. Luteoviruses levi-like lineage 9. Luetovirus aphid transmission and biology 10. Geminiviruses 11. Caulimoviruses 12. Rhabdovirus plant-animal viruses 13. Tospoviruses, new vir uses 14. Satellite viruses/RNAs and viroids 15. Viruses and biotechnology. PLP 202D. Advanced Plant Pathology: Mycology. 2 units, Three 1 hour lectures, one 1 hour discussion per week Topics: 1. Introduction to fungi – fungi as modular organisms 2. Fungal systematics – key groups of fungal pathogens and how they are grouped in a modern sense 3. Fungal identification - morphology to molecular approaches 4. Species concepts: morphological, biological, phylogenetic 5. Species concepts (continued) 6. Sexual compatibility 7. Somatic compatibility 8. Fungal growth 9. Fungal morphogenesis 10. Fungal biology- spores, dormancy and spore dispersal 11. Fungal nutrition and metabolism 12. Genetic manipulation of fungi 13. Genomic analyses of plant pathogenic fungi 14. Fungal population biology in the field 15. Fungal population biology in the field Fall Quarter (Year 2): 2 modules, each 5 weeks PLP 202E Advanced Plant Pathology: Host-Parasite Interaction:. 2 units, Three 1 hour lectures, one 1 hour discussion per week Topic: 1. History and introduction to types of interactions. 2. Pathogenicity and virulence: Life on the surface 3. Pathogenicity and virulence: Breaching the cell wall 4. Pathogenicity and virulence: Biochemical and physiological weapons: Hormones, enzymes and toxins 5. Pathogenicity and virulence: Pathogen effectors and effector delivery systems 6. Host defenses: Biochemical – preformed and induced cell wall defenses. 7. Host defenses: Biochemical – preformed and induced metabolites and enzymes 8. Host defenses: Biochemical – Local and Systemic responses 9. Host responses: Pathogen recognition – Innate immunity and MAMPs 10. Host responses: pathogen recognition – NBS-LRR genes and effector triggered immunity. 11. Evolution of host-pathogen specificities: Host R genes/Pathogen Effectors 12. Technical approaches used to study the interaction between host and pathogen Genomic, proteomic, microarrays PLP 202F. Advanced Plant Pathology: Disease Control. 2 units, Three 1 hour lectures, one 1 hour discussion per week Topics: 1. Underlying epidemiological principles in disease management 2. Underlying epidemiological principles in disease management (cont.) 3. Six major strategies for disease control: PARTEE 4. Specific methods for reducing initial disease: exclusion, physical and chemical techniques 5. Chemical control: historical perspectives; selective toxicity; experimental design considerations for laboratory, greenhouse, and field tests of fungicidal action 6. Chemical control: epidemiology of fungicidal action and formulation issues 7. Chemical control: selected classes, modes of action and uses, chemistry of systemicity 8. Managing pathogen resistance to fungicides 9. Plant activators: theory and reality 10. Successful disease forecast and risk assessment models 11. Biologicals in disease control and comparative efficacy with conventionals 12. Deploying host resistance genes 13. Examples of GMO-based disease control: societal acceptance and resistance (Papaya RSV; coat protein; etc.) 14. Regulatory biology and disease management: who does it and when do you call them?; quarantine and pest exclusion; detection and diagnostic networks 15. What’s on the horizon – cutting edge technologies and approaches Fall, concurrent with.202E and F. PLP 220, Plant Pathology Laboratory. 2 units, Two 3 hour laboratory sessions per week Topics: 1. Koch’s postulates. Three different pathogens – one virus and one cellular pathogen – to be taken through all appropriate steps. This would include isolation and the use of selective media in the case of bacteria and fungi, and identification of the pathogen by appropriate means. The relative merits of alternative inoculation methods and disease rating scales would also be considered, along with limits on interpretation of the results. 2. PCR and sequence analysis. This would include a consideration of the theoretical basis for PCR, a detailed evaluation of the reaction components and how they influence the amplification process. Commonly used variants such as Q-PCR would also be covered. Sequence analysis would include an introduction to data base searches and the use of software for alignments and phylogenetic analysis. 3. Experimental design: with an emphasis on issues important to plant pathology. 4. Microscopy (potential) Other required Plant Pathology Courses for all students: Field course: PLP 205 or PLP 206 Seminar requirements will remain the same (as per our current grad student guidelines): All MS students must enroll in the Plant Pathology Departmental general seminar (PLP 290) each quarter unless there is a scheduling conflict with another course. In addition, students must enroll in at least one offering of the other special Plant Pathology seminars (e.g. PLP 291, PLP 295, NEM 290 etc.) during each year of their enrollment in graduate school. It may be possible to satisfy this requirement by enrolling in another participatory seminar course offered by another Department; discuss this with your Adviser. These special seminars vary in topic and availability and will be announced in communications to graduate students one or two quarters in advance of their presentation and by publication in the UC Davis Class Schedule and Registration Guide.” M.S. degree The core curriculum fills all course requirements = 23 units However, the existing total unit requirement as stated in our grad student hand book would remain the same: “Plan I. The student must complete at least a total of 30 units of upper division undergraduate and graduate courses (includes the plant pathology required courses; 12 units must be strictly graduate work) and submit and gain approval of a master's thesis.” Ph.D. In addition to the core curriculum, two additional elective courses would be required. The following Nematology courses: 201, 203, 204, 205, and 206 would be among the options, as they are currently.