Electrophoresis
Timothy G. Standish, Ph. D.
©2000 Timothy G. Standish
O HO P O CH2 O
H
N O O N O
OH
H
P HO
HO
O
O
O
H
NH2
O
D N A
OH HO P O CH2 O O N N
NH2
N
H
OH
N
O
CH2 O
O HO P O CH2 O O
H
O
P OH
OH
O
N N N NH
H
H2O
NH2
O
CH2
O
P H
OH
H2O
CH2
G T A
A C G C
-
-
-
-
-
G C T A C G A T Major groove A T C G G C T A
-
-
-
-
-
Minor groove
-
-
-
-
-
-
-
The Watson - Crick Model Of DNA
-
T
-
-
-
-
-
©2000 Timothy G. Standish
Submarine Gel Electrophoresis
Sample to be loaded
Negative electrode
Gel Box
Positive electrode
Well
Gel
©2000 Timothy G. Standish
Submarine Gel Electrophoresis
©2000 Timothy G. Standish
Submarine Gel Electrophoresis
100 V
DNA Migration
+
©2000 Timothy G. Standish
Gel Electrophoresis
Wells
©2000 Timothy G. Standish
Gel Electrophoresis
Wells
©2000 Timothy G. Standish
Gel Electrophoresis
Wells
Large
Direction of DNA Travel
Small
+
©2000 Timothy G. Standish
Electrophoresis of Dyes
1 2 3 4 5
©2000 Timothy G. Standish
©2000 Timothy G. Standish