Poster Presentation 6-20 Inulin Containing Biomass for Bioethanol Production: Fructose Extraction Methods and Fermentation Assays M.José Negro, Ignacio Ballesteros, Paloma Manzanares, J.Miguel Oliva, Felicia Sáez, Mercedes Ballesteros* Renewable Energies Department-CIEMAT Avda. Complutense, 22 28040-MADRID SPAIN Phone: +34 91 346 6262 email: firstname.lastname@example.org Jerusalem artichocke (Helianthus tuberosus) is a perennial herbaceous plant that develops underground stolons forming shaped tubers which are similar to potatoes. The tubers consist of 75-79% water, 2-3% proteins and 15-16% carbohydrates, of which the D-fructose polymer inulin can constitute 8% or more. This D-fructose polymer can be used to produce ethanol or to obtain chemicals for pharmacy, fine chemistry or materials industry. The carbohydrates, which are initially in high levels in the stems, have been transferred to tubers by the end of the growing cycle. The utilization of tubers to obtain inulin presents some drawacks as it is the high percentage of water which may promote the risk of contamination under storage conditions and the need for deep tillage to obtain the tubers. On the other hand, the possibility to harvest the above-ground biomass before tubers development and use the inulin containing stalks as feedstock for ethanol production is now envisaged as an interesting option to this species mostly used for animal feeding. In this work the extraction conditions of inulin from Jerusalem artichocke stalks have been studied and optimized in order to attain a high fructose content fermentable extract. Furthermore, fermentation of those extracts using Kluyveromyces marxianus CECT 10875 and baker´s yeast has been performed and the potential to use this feedstock for bioethanol production assessed. Reults from these experiments will be presented.
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