Effect of neem seed and kernel extracts on individuals
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240- EFFECT OF NEEM SEED AND KERNEL EXTRACTS ON INDIVIDUALS OF Cephalobus persegnis [EFEITO DE SEMENTES DE NEEM E EXTRATOS DE KERNEL EM INDIVÍDUOS DE Cephalobus persegnis] Ardakani, A.S.1; Gaur, H.S.2; Kamara, A.2 1Agricultural and natural resources research center of Kohgyloyeh va Boyreahmad province (Iran) 2Division of Nematology, IARI, New Delhi, 110012, India. Email: firstname.lastname@example.org Active individuals of C. persegnis were treated in vitro with aqueous extracts of neem seed kernel (NSKE) and naem seed (NSE) at 0 (control), 1, 2 and 4% w/v for 24 and 48 h to observe immobility and mortality, if any. At a concentration of 1%, the NSKE caused immobility of 19.9% of the C. persegnis exposed for 24 h which increased to 21.3% by 48 h. Some of immobile nematodes were reactivated when transferred to water, hence the mortality caused due to 24 and 48 h exposure was 16.3 and 19.6%, respectively. At lower dilution of 2%, the NSKE resulted in significantly lesser immobility of 33.1% at 24 h which increased to 36.1% at 48 h causing per cent mortality of 26.3 in 24 h and 24.8% at 48 h, with a revival of 37.7 and 75.2% after 24 h and 48 h exposure, respectively. NSKE at 4% caused an immobility of 52.7 at 24 h which increased to 57.5% at 48 h. The revival in mobility on placing them in water for 24 h was about 56%, in individuals treated for 24 h resulting on mortality of 44.1%. The revival in individuals kept in NSKE for 48 h was 52.3% resulting in a mortality of 47.7%. Thus, the different dosages of NSKE showed significant differences in per cent immobility and mortality caused in C. persegnis. The NSE at 4% caused an insignificant immobility of 3.9% at 24 h and 3.6 per cent at 48 h. All these individuals when placed in distilled water revived within 24 h. No effect on mobility was observed in individuals at lower dilutions of 2% or 1%.