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Title Page ELSEVIER THE POTENTIAL BIODEGRADING

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Title Page ELSEVIER THE POTENTIAL BIODEGRADING Powered By Docstoc
					   THE POTENTIAL BIODEGRADING ABILITY OF Xylaria species ON
NATURAL RUBBER, CHICKEN FEATHER, AND POLYSTYRENE THROUGH
             SCANNING ELECTRON MICROSCOPY




            Mary Bernadette Vallesfin Eglosoa, Janine Erica Padilla Dayaob,
               Mary Ann T. Tavanlarc and Anna Theresa A. Santiagod

        a
           marybernadetteegloso@gmail.com; (+63.053) 327-3714; +63.915.383.1788
                                    b
                                      jana_erica99@yahoo.com
  a,b,d
        Department of Biology, College of Arts and Sciences, University of the Philippines
                    Manila, Padre Faura St., Ermita, Manila, Philippines 1000
a,b
    Phi Sigma Biological Honor Society, Alpha Chi Chapter, University of the Philippines
                              Diliman, Quezon City, Philippines 1104
         c
           National Institute of Molecular Biology and Biotechnology, University of the
                    Philippines Los Baños, College Laguna, Philippines 4031




                                      SUMMARY

         The potential biodegrading ability of the Xylaria sp. strains on three pollutants,
namely, natural rubber, chicken feather and polystyrene were determined in terms of
colonization. The fungal strains namely the wild type, SDM (sterile dark mycelia) and the
five mutant strains (PNL 114, PNL 116, PNL 118, E26 and E35) were cultured in a pH
5.0 mineral medium with 0.5% glucose as carbon source, while the three pollutant
substrates as co-carbon source, and stored at 250C. Scanning Electron Microscopy (SEM)
revealed colonization of Xylaria sp. strain on the three pollutants. Generally, the black
strains, namely the SDM wild type, E26 and E35, have been observed to possess a higher
potential to biodegrade natural rubber, chicken feather and polystyrene than the albino
mutants PNL 114, 116 and 118. The black variant strain E26 has shown the greatest
biodegrading potential for biodegrading natural rubber. Polystyrene was effectively
colonized by all strains. Chicken feathers were colonized by the SDM and E35 but may
require a longer incubation period for degradation.




    a
        #135 Phase 4 Block 5 Lot 18, Our Lady of Lourdes Avenue, V&G Subdivision,
                          Tacloban City, Leyte, Philippines 06500
              b
                1363 Dahlia St., Nichols, Pasay City, Manila, Philippines 1300