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					Legends for supplemental figures

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Supplemental Figure 1.          Fe translocation from the roots to the shoots in Fe-deficient
                                                                           52
and Fe-sufficient barley (Experiment 2). A, B, Images of                        Fe translocation in
Fe-deficient (A) and Fe-sufficient (B) barley using BAS-1500. Rectangles show the area
measured by PETIS. C, D, PETIS images of 52Fe accumulation in Fe-deficient (C) and
Fe-sufficient (D) barley after 6 h. E, Time course of radioactivity accumulation in
Fe-deficient barley analyzed using PETIS. The images are shown at 15- and 30-min
intervals (0–60 and 60–360 min, respectively). Data were scored every 3 min. F, Time
course of radioactivity accumulation in the DC of Fe-deficient and Fe-sufficient barley
measured by PMPS. The maximum measured radioactivity was defined as 100.
Activities are shown as percentages of the maximum value. Scale bar = 4 cm.

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Supplemental Figure 2.          Fe translocation from the roots to the shoots in Fe-deficient
                                                                           52
and Fe-sufficient barley (Experiment 3). A, B, Images of                        Fe translocation in
Fe-deficient (A) and Fe-sufficient (B) barley using BAS-1500. Rectangles show the area
measured by PETIS. C, D, PETIS images of 52Fe accumulation in Fe-deficient (C) and
Fe-sufficient (D) barley after 4.5 h. E, Time course of radioactivity accumulation in
Fe-deficient barley analyzed using PETIS. The images are shown at 15- and 30-min
intervals (0–60 and 60–270 min, respectively). Data were scored every 3 min. F, Time
course of radioactivity accumulation in the DC of Fe-deficient and Fe-sufficient barley
measured by PMPS. The maximum measured radioactivity was defined as 100.
Activities are shown as percentages of the maximum value. Scale bar = 4 cm.


Supplemental Figure 3. 52Fe translocation in Fe-deficient barley in the dark and in the
                                            52
light (Experiment 2). A, B, Images of            Fe translocation in Fe-deficient barley in the
dark (A) and in the light (B) using BAS-1500. Leaf numbering: 4, youngest leaf (YL);
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3, third leaf; 2, second leaf; 1, first leaf. C, D, Time course of         Fe translocation in the
DC (C) and at the points indicated in A and B (D) of Fe-deficient barley measured by
PMPS and PETIS, respectively. The maximum measured radioactivity in PMPS and
PETIS was defined as 100. Activities are shown as percentages of the maximum value.
E, 52Fe content in the leaves of Fe-deficient barley after 6 h.



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Supplemental Figure 4. 52Fe translocation in Fe-deficient barley in the dark and in the
                                                  52
light (Experiment 3). A, B, Images of                  Fe translocation in Fe-deficient barley in the
dark (A) and in the light (B) using BAS-1500. Leaf numbering: 4, youngest leaf; 3,
third leaf; 2, second leaf; 1, first leaf. The youngest leaves are included in the third leaf
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in A and B. C, D, Time course of                Fe translocation in the DC (C) and at the points
indicated in A and B (D) of Fe-deficient barley measured by PMPS and PETIS,
respectively. The maximum measured radioactivity in PMPS and PETIS was defined as
100. Activities are shown as percentages of the maximum value. The points II and IV
indicate the measured positions of the youngest leaves. The third leaf and the youngest
                             52
leaf were separated in the        Fe measurement, although the youngest leaves are seen to
be included in A and B. E, 52Fe content in the leaves of Fe-deficient barley after 6 h.

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Supplemental Figure 5.       Effect of heat-girdling on              C-photoassimilate translocation
from the leaf in barley (Experiment 2, 3). A, C, Gross images of steam-treated barley. B,
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D, Images of the distribution of           C in steam-treated barley detected by a BAS-1500.
Arrowheads indicate the position of steam treatment. Rectangles indicate the 11CO2-fed
region. Scale bar = 4 cm.


Supplemental Figure 6. Effect of heat-girdling on 52Fe translocation from the roots to
the shoots in Fe-deficient barley (Experiment 2). A, C, Gross images of steam-treated
                                                                               52
(A) and untreated (C) barley. B, D, Images of the distribution of                   Fe in steam-treated
(B) and untreated (D) barley detected by a BAS-1500. Arrowheads indicate the position
of steam treatment. Leaf numbering: 3, youngest leaf (YL); 2, second leaf; 1, first leaf.
Scale bar = 4 cm. E, The ratio of the 52Fe radioactivity of the upper region to that of the
lower region after heat-girdling the second and third youngest leaves.


Supplemental Figure 7. Effect of heat-girdling on 52Fe translocation from the roots to
the shoots in Fe-deficient barley (Experiment 3). A, C, Gross images of steam-treated
                                                                               52
(A) and untreated (C) barley. B, D, Images of the distribution of                   Fe in steam-treated
(B) and untreated (D) barley detected by a BAS-1500. Arrowheads indicate the
steam-treated region. Leaf numbering: 3, youngest leaf (YL); 2, second leaf; 1, first



                                                                                                     2
leaf. Scale bar = 4 cm. E, The ratio of the 52Fe radioactivity of the upper region to that
of the lower region after heat-girdling the second and third youngest leaves.




Legends for supplemental movies


Supplemental movie 1. 52Fe translocation from the roots to the shoots in Fe-deficient
(left) and Fe-sufficient (right) barley. (Left) Time course of radioactivity accumulation
analyzed using PETIS. The images were obtained at 3-min intervals. (Right) Gross
image of Fe-deficient and Fe-sufficient barley analyzed using PETIS.


Supplemental movie 2. 52Fe translocation in Fe-deficient barley in the dark (left) and
in the light (right). (Left) Time course of radioactivity accumulation analyzed using
PETIS. The images were obtained at 3-min intervals. (Right) Gross image of
Fe-deficient barley analyzed using PETIS.




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