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					            NSF-NIEHS
             Centers for
      Oceans and Human Health
- Novel integration of NIH and NSF.
- Stimulated by scientific community,
  and by CORE, an NRC report, and
  the Directors of NSF and NIEHS.
- Focus on HAB, pathogens, pharmaceuticals,
- Integrate biological / oceanographic approaches.
            NSF-NIEHS
             Centers for
      Oceans and Human Health
- Joint Request for Applications (RFA)
- Competitive review of Center grant
applications.
- 20 plus applications
- Four Centers funded:
     -Washington
     -Hawaii
     -Woods Hole
   Woods Hole Center
           for
Oceans and Human Health

Integrated effort by three Institutions:

 Woods Hole Oceanographic Inst.
  Marine Biological Laboratory
 Massachusetts Inst. of Technology
Woods Hole Center for Oceans and Human Health

                                  COHH Director
                                   J. Stegeman
                                  Deputy Director
                                  D. Mcgillicuddy

                     Internal Advisory       External Advisory
                        Committee               Committee

                      Administrator         WHOI Administration



             RESEARCH                      GENOMICS                 PILOT
             PROJECTS                    FACILITY CORE            PROJECTS

  D. Anderson         D. Mcgillicuddy
   D. Erdner                               M. Sogin               S. Hajduk
                                          H. Morrison             M. Hahn
     R. Gast               M.Polz
 L. Amaral-Zettler       J. Lerczak
       Research Focus
  Harmful algal blooms (2 projects)
    Infectious agents (2 projects)

      Temperate Coastal Waters

New England waters as a model system.
              Woods Hole
  Center for Oceans and Human Health
                       Administrative Core
       Stegeman                              McGillicuddy



 Advanced                 Population               Coastal
 Genomics                  Biology              Hydrodynamics
 Core Facility         1. Anderson: HABs 2. McGillicuddy: HABs
Sogin Morrison         3. Gast and Amaral-Zettler: Infectious Diseases
                          4. Polz and Lerczak: Infectious Diseases



                        Pilot Projects
    Director: Hajduk                     co-Director: Hahn
             WHOI

    MBL                  MIT

          Woods Hole
           Center for
          Oceans and
          Human Health

                          Other
 EHS
                         Institutes
Centers
           Other COHH    & Centers
    Human Pathogens in the Environment



      -reservoirs
?     -favorable growth
      -Gene transfer




environmental                              Shellfish poisoning,
 amplification            transport           toxicosis….


        understanding ecology = understanding risk
                --> prediction and prevention
        Mt. Hope Bay

 Heavily impacted by humans
   – Sewage outfalls, industrial
   waste, thermal discharge

Mt. Hope Bay Natural Laboratory
  – Centralized data bases
  – Predictive current models under
  development
  – No microbial or pathogen
  sampling program
 Proxy for other New England bays
                  Vibrio infections - a worldwide problem


           Environmental reservoirs,                            WH-COHH Vibrio project
           routes of transmission and infection
                                                                    • Understand
                                                                    • Predict
                      Wound, eye and ear infection:                 • Protect
                      - rising worldwide
                      - numbers not well known
                                                                - Environmental prevalence
                                                                  and growth factors
                                                                - Gene transfer and
                                                                  evolution
                                                                - Risk assessment
    V. vulnificus
V. parahaemolyticus
                       Seafood consumption:
                       - up to 10,000 infections/yr in US and
                         reports of infection rising (CDC);
                       - 95% of all seafood related deaths
                      Human Pathogens:
                  Presence & Persistence
            in the Coastal Marine Environment

 Studies of traditionally non-marine human
  pathogens in coastal marine waters:
       Essential to determine their potential
  to impact human health

 Evidence and vectors
    Sea otters, whales, and shellfish can            (Giardia)
      harbor protistan pathogens such as
      Giardia and Cryptosporidium
   – Shellfish and protists (such as
      Acanthamoeba) can harbor bacterial
      pathogens such as Legionella and
      Vibrio, and enhance pathogenicity.

                                                 (Legionella in amoeba)
HAB threats to human health in temperate waters of the
                  northeastern U.S.
      Woods Hole COHH HAB projects
• Overall objective: to link dinoflagellate population structure,
  physiology, and toxicity in order to improve prediction and
  prevention of HABs.

• Rationale: cellular toxicity varies with environmental and
  genetic factors; cell number is not always a good proxy for
  toxicity


    Genotype
                     Physical-biological
   Physiology        coupled model



                                                    Toxicity
              Paralytic shellfish poisoning (PSP)

•   causes paralysis and death;
•   causative organism Alexandrium fundyense (dinoflagellate);
•   widespread and recurrent annual problem;
•   ongoing shellfish monitoring programs in ME, NH, MA, CT
Germination   Germination, Growth   Germination, Growth,
   Only            &Mortality       Mortality & Nutrient lim.




May


Jun



Jul



Aug
Woods Hole Center for Oceans and Human Health




         Pilot Project Program


         Steve Hajduk (MBL), Director
       Mark Hahn (WHOI), Deputy Director




      2004 Call for Proposals
                Pilot Project Program


                Objectives

• Support short-term projects to assess feasibility of
  new research topics
• Generate preliminary data
• Expand scope of Center research
• Expand participation in Center
                       Pilot Project Program



               Areas of emphasis

       • Harmful algal blooms (HABs)
       • Water- and vector-borne diseases
       • Marine-derived pharmaceuticals and probes


RFP: http://grants1.nih.gov/grants/guide/rfa-files/RFA-ES-03-003.html
NRC/NAS (1999) From Monsoons to Microbes: Understanding the Ocean’s
 role in Human Health
NSF/NIEHS Oceans and Human Health roundtable report, December 2001
                Pilot Project Program


                  Eligibility

• MBL, MIT, WHOI Scientific and Sr. Technical Staff
             Pilot Project Program

              Budgets
• up to $50K direct costs
• 1 year + no-cost extension if needed
• Earliest start date: October 1, 2004

• Salaries
• Supplies
• Student stipend and tuition

• Equipment - not usually
• Travel - meetings: No
• Travel - sample collection: If strongly justified
• Ship time - if strongly justified
              Pilot Project Program


            Pre-proposal
                 (required)

• Due July 15, 2004
• 1 page summary of project
• Direct and indirect costs
• 2p CV/Biosketch for PI and other key personnel
   (NSF or NIH format)
• Submit electronically:


Steve Hajduk, MBL (shajduk@mbl.edu) or
Mark Hahn, WHOI (mhahn@whoi.edu)
                          Pilot Project Program

                     Full proposals
• Due August 15, 2004 at 5 PM EDT.
• Title page
• Research Plan (5 pp.)
        a) Objectives
        b) Background & Rationale
        c) Experimental design and methods
        d) Facilities available and/or use of genomics core
        e) Relevance to COHH program
        f) Broader impacts (see NSF guidelines)
        g) References (not included in 5p limit)
• Budget (Direct and indirect costs)
• Budget justification (1 p)
• 2p CV/Biosketch for PI and other key personnel(NSF/NIH format)
• Other support (Key personnel; NIH or NSF format)
• Human subject / Vertebrate animal approvals
• Submit hard copy (1) and electronically
                     Pilot Project Program


                        Review

•   Phase I
    - Woods Hole COHH Internal Advisory Committee (IAC)
    - outside reviewers (EAC) as needed
    - Written critiques

•   Phase II
    - presentations (10 min) to IAC
               Pilot Project Program


        Review criteria


• Scientific merit
• Relevance and potential impact
• Innovation
• Investigators
• Facilities
• Broader impacts
 Pilot Project Program




Questions?
Model run: cyst
germination plus
growth and mortality


Present model assumes
that all Alexandrium
fundyense cells are the          QuickTime™ and a
                                BMP decomp resso r
                          are neede d to see this picture.


the same (i.e., same
growth rate, nutrient
responses, toxicity,
motility, etc.)


Will address different
strains known to exist.
        Woods Hole COHH
• Addressing public health issues.
• Incorporating advanced genomic studies
  and oceanography.

				
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