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INSTRUCT Powered By Docstoc
Integrated Structural
Biology Infrastructure
for Europe
INSTRUCT Integrated Structural Biology Infrastructure for Europe

The mission
INSTRUCT aims to provide open access to world-class
integrated structural biology infrastructure to support
European biomedical research

Advance and disseminate technologies and
methodologies, driven by strong in-house biomedical
research programmes

Train researchers throughout Europe so as to optimize
exploitation of the INSTRUCT infrastructure at their
INSTRUCT – the vision
The intention is to establish, over the next decade, an Integrated Structural Biology
Infrastructure to support development of Cellular Structural Biology as a cutting edge
tool for integrating individual macromolecules and complexes into higher-order
structures. This will open new scienti c horizons and underpin a transformation in
biomedical research. To meet this challenge will require seamless synergy of techniques
providing information over a wide range of resolutions and time-scales.
INSTRUCT is one of the biomedical projects in the European Strategy Forum on Research
Infrastructures (ESFRI). It aims to establish a number of centres across Europe, which
will not only provide infrastructure but also engage in development of instrumentation,
technologies and methodologies, as well as in coupled basic research. These core centres
will provide pan-European user access to state-of-the-art equipment covering the major
techniques of structural biology. By talking to European industry with a coherent voice,
ties will be forged which will promote the commercialization of innovative technologies.
Associate centres will provide infrastructure and
                                                       From Atom to Cell
stimulate technology development in emerging           Spheres demonstrate loss of resolution
The crucial need for such infrastructures arises                           Single crystal X-ray
                                                                           di raction (&NMR)
out of major technological developments which
                                                                            Single particle EM
have increased the complexity, and hence the                                reconstruction
cost, of cutting edge equipment in structural                                 Isolated particle
biology, which is now beyond the nancial means                                EM tomography
of most individual laboratories. This applies
across the board, from protein production,                                        Cellular EM
through NMR and X-ray crystallography, to
                                                                                 Cellular X-ray
electron microscopy. This will require a major and                               tomography
focused investment in order for European science                   And then…
                                                              modeling to put pieces
to maintain its competitive edge and to play a                       together
leading role in a vital research area.
INSTRUCT Integrated Structural Biology Infrastructure for Europe

INSTRUCT – the preparatory phase
The preparatory 2-year phase of INSTRUCT, starting in April 2008, is developing mechanisms for establishing
and maintaining a pan-European structural biology infrastructure composed of a number of Core Centres,
reinforced by a number of Associate Centres with complementary expertise. In parallel, National User Groups
are being established, which will help to determine the strategic priorities of INSTRUCT, establish guidelines
for access and consider establishing National Centres. The development of cutting-edge infrastructures
will be energized by establishing ‘in-house’ focus on a few key biomedical and environmental challenges.
Strong involvement of European industry and SMEs will occur through consultation and collaboration in
feasibility studies. Such ties will arise directly out of the requirement of INSTRUCT for high-end innovative
instrumentation which will challenge European industry to improve its capabilities. Furthermore, the use
of INSTRUCT facilities will strengthen European industrial competitiveness, in particular in Biopharma and
Agrochem companies. INSTRUCT will establish a training program aimed at enabling scientists throughout
Europe to take full advantage of the infrastructures provided. To permit a smooth transition to the construction
and operational phases, the preparatory phase will establish a framework for governance as well as a
mechanism for de ning participants. Furthermore, a nancial and legal framework will be set up which will
enable major funding bodies to work together to provide a coherent infrastructure with wide European access.

The six biomedical infrastructures that are part of ESFRI:

   Biobanks                INSTRUCT
 Biomolecular              Structural           INFRAFRONTIER            Translational              Clinical
  Resources                 Biology              Animal models                                     Research

Novelty & Impact
INSTRUCT will establish a Pan-European infrastructure for Integrated Structural
Biology based on new centres and upgrades of existing ones. These centres will
develop complementary expertise and instrumentation, depending on their areas
of focus, while maintaining and making available a set of core technologies. In the
wet lab these may include protein/macromolecular complex production using
parallel technologies, as well as the generation of organelles, cellular samples and
functional characterization. Each core centre will provide access to cutting-edge
infrastructure for methods including protein production, crystallization, mass
spectrometry, biophysical methods, NMR, X-ray crystallography, electron microscopy
and tomography, X-ray imaging and relevant aspects of light microscopy as well as
computational methods. Centres will have interlocking biomedical research themes.
They will thus develop complementarities, taking into account originality, the
importance of the biological questions addressed, and their relevance to European
priorities, such as human health and quality of life, the environment, therapeutic
innovation and biotechnology. All centres will be fully open to the academic and
industrial communities, and will provide, on a project basis, access to production and
experimental facilities. INSTRUCT will thus provide a structural biology platform for all
aspects of biological research in the 21st century.
INSTRUCT Integrated Structural Biology Infrastructure for Europe

O   University of Oxford                                 O   Centre National de la Recherche Scienti que
O   CERBM Strasbourg                                     O   Israel Ministry of Science Education and Sport
O   European Molecular Biology Laboratory                O   Max-Planck-Society
O   Consorzio Interuniversitario Risonanze               O   Bundesministerium fuer Bildung und Forschung
    Magnetiche di Metalloproteine Paramagnetiche
                                                         O   Bruker BioSpin GmbH
O   Weizmann Institute of Science
                                                         O   Consiglio Nazionale delle Richerche
O   Medical Reseach Council

Affiliated Countries
A liation is the mechanism by which a country or
region became part of INSTRUCT. A liated countries
have expressed their support for the project and
have named a point of contact for INSTRUCT within
their scienti c institutions and funding bodies. The
scienti c institution named as contact point will be
the base of the country national users group.
The following countries are a liated to INSTRUCT
(January 2009)
Czech Republic, Denmark, Finland, France, Germany,
Greece, Hungary, Israel, Italy, Netherlands, Portugal,
Slovakia, Slovenia, Spain, Sweden, Switzerland,
United Kingdom

           A liated countries
           Countries in the process of a liation
           Countries that just started involvement
INSTRUCT Working groups
All working groups have started to function and rst reports are available at
O   Legal & governance and nancial framework | Dino Moras (CERBM, France),
    Frances Rawle (MRC, UK), David Stuart (University of Oxford, UK).
O   Building & Access framework and Resource Identi cation | Ivano Bertini
    (CIRMMP, Italy), Stephen Cusack (EMBL Grenoble, France), David Stuart (University
    of Oxford, UK), Joel L. Sussman (Weizmann Institute, Israel).
O   Outreach Framework | Lucia Banci (CIRMMP, Italy)
O   Production of biomolecules and their complexes | Dino Moras (CERBM, France).
O   Large scale virus production | Dennis Bamford (Helsinki University, Finland).
O   Biophysical characterization and crystallization of macromolecules for
    Structural Biology experiments | Anastassis Perrakis (NKI, Netherlands).
O   Mass spectrometry | Albert Heck (Utrecht University, Netherlands), Carol
    Robinson (University of Cambridge, UK).
O   Systems biology | Luis Serrano (Centre de Regulacio Genomica, Spain).
O   Computational structural biology | Keith Wilson (University of York, UK).
O   Solid State NMR | Hartmut Oshkinat (Leibnitz Institute, Germany).
O   NMR | Vladimír Sklenárˇ (Masaryk University, Czech Republic).
O   Characterisation of unstructured proteins | Peter Tompa (Hungarian Academy
    of Sciences, Hungary).
O   Electron microscopy | Wolfgang Baumeister (Max-Plank Institute, Germany).
O   Developments in image processing | Jose Maria Carazo (CNB-CSIC, Spain).
O   X-ray imaging | Colin Nave (Diamond, UK).
O   Synchrotron based macromolecular crystallography | Gunter Schneider
    (Karolinska Institutet, Sweden).
O   Light microscopy | Christoph Mueller (EMBL Heidelberg, Germany).
O   Complementary techniques | Matthias Wilmanns (EMBL Hamburg, Germany).
INSTRUCT Integrated Structural Biology Infrastructure for Europe

Project Coordinator:
Prof. David Stuart

                                                                   Desing, Photography & Printing dept., Weizmann Institute of Science
Project Manager: Dr. Claudia Alen Amaro
Wellcome Trust Centre For Human Genetics
University of Oxford
Roosevelt Drive, Oxford OX3 7BN
Tel: +44 (0) 1865 287808