Produced and edited by

							                                                                                                             2007



  Along with the mass production of genetically engineered mice in late years, reproductive
engineering techniques have become inevitable as one of the related technologies for producing
the mice. However, it was almost impossible to learn these techniques perfectly from the existing
manuals because the reproductive engineering consists of manipulation under stereoscopic
microscopes. This time, we have created the “Manual for Reproductive Engineering Techniques”
on CD-ROM which data is HTML format, with a lot of moving images, illustrations and photographs
(both in Japanese and in English available). This CD-ROM shows the preparation of media and
stock solution, caesarean section and fostering, as well as IVF, cryopreservation of embryos and
sperms and the techniques of an embryo transfer. You can easily learn the techniques with this
CD-ROM, for each point of each manipulation is given an explanation with moving images. This
CD-ROM will contribute to improve your reproductive engineering techniques for genetically
engineered mice.

                 Contents
                 ・Preparing and Assembling Pipettes for Embryo Handling
                 ・In Vitro Fertilization
                 ・Simple Vitrification of Mouse Embryos
                 ・Vitrification and Transplantation of Mouse Ovary
                 ・Cryopreservation of Mouse Spermatozoa
                 ・Partial Zona Dissection (PZD)
                 ・Collecting Two-Cell-Stage Embryos
                 ・Transplantation of Mouse Oviducts Containing 2-Cell Embryos at Low Temperature
                 ・Vasectomy for the Creation of Sterile Males
                 ・Embryo Transfer into the Oviduct
                 ・Production of Chimeric Mice by 8-Cell Aggregation
                 ・Embryo Transfer into the Uterus
                 ・Caesarean Section and Fostering
                 ・References

Price (including tax) : ¥5,250, Product code : RETM-J (Japanese Ver.) / RETM-E (English Ver.)


 Produced and edited by ：
      Naomi Nakagata
       Division of Reproductive Engineering,
       Center for Animal Resources and Development (CARD),
       Kumamoto University

                                                            Please contact our Sales Agency below when you order.
Released by ：                                                 Sales Agency ：



    Transgenic Inc.                                               Funakoshi Co., Ltd.
                                                                   E-mail： reagent@funakoshi.co.jp
                                                                   TEL： +81-3-5684-1620 FAX： +81-3-5684-1775
     URL： http://www.transgenic.co.jp                              URL： http://www.funakoshi.co.jp