OSRW PROTOCOLS – Cumberland blackside dace (Phoxinus cumberlandensis) Revision date 4/12/2003 The following is a list of KPDES requirements that are to be performed before land preparation, mining or any mining related disturbances that takes place within the watershed of any outstanding state resource water (OSRW) that supports a population of the Cumberland blackside dace, Phoxinus cumberlandensis. Applicable general KPDES and DSMRE requirements are listed first with the specific requirements of the KPDES Special Conditions Section for the two year pre-mining biological community assessments being listed second. The requirements are as follows: 1) Pre-mining biological community assessments and physical-chemical sampling shall be required. Methods for this monitoring shall be performed according to Division of Water's standard operating procedures (SOP) manual. 2) No instream disturbances shall occur in the OSRW in any blue-line stream as delineated by the U.S.G.S 7.5 minute Quadrangle Map unless approved by the Division. 3) A 100 foot buffer zone shall be maintained for each stream including tributary streams. Width variances of the buffer zone may be granted on a case by case basis. Streams within the watershed of the OSRW shall not be used for sediment control or permanent material storage without prior approval from the Division of Water, DSMRE and U.S. Department of the Interior, Fish and Wildlife Service. 4) All underground mining within the immediate watershed of an OSRW shall adhere to a minimum of 150 foot buffer under the subsidence dome so that no stream will be impacted by loss of flow and habitat. The following are the specific conditions required for these streams: 1) A comprehensive study plan shall be submitted to the Division of Water before sampling takes place in the stream. The study plan shall be approved before sampling takes place. If a written approval of the study plan is needed, please state this requirement in the cover letter. 2) The initial fish survey (can be the first survey in order to save time) shall be conducted in September, October or November of the first year. These months are selected so as not to cause stress or mortality to the dace during a late breeding season. The enumeration of the population and identification of the segments of the stream that support spawning habitat throughout the mining area and at a control site shall be included. These streams shall be partitioned into segments of between 100 to 300 m in length for dace population sampling. These segments shall be identified by flagging, so that the same sampling effort can be duplicated in the future. A visual survey during April or May could be warranted and will be conducted during breeding season to identify areas of breeding and nest compatibility with the associated species (i.e. Semotilus and\or Campostoma). These areas should be delineated for further benthic substrate particle size analysis if so warranted. 3) Delineation of the dace size classes in total length and general health and condition shall be included in the assessment. Information shall be recorded on a separate data sheet and included in the report. 4) Water quality sampling sites shall be established on the mainstem between each tributary that may be affected by mining. A control site shall be included above all disturbances in the watershed. Each tributary shall also be monitored for water quality parameters. These sampling sites may coincide with the aquatic macroinvertebrate sites which shall be required in the second year. The water quality survey sites shall be sampled biweekly. The parameters sampled instream will include flow (cfs), dissolved oxygen, pH, conductivity, temperature, alkalinity, acidity, total settleable solids, total suspended solids, turbidity (NTU's), total recoverable iron, total recoverable manganese, total dissolved iron and total dissolved manganese. Rainfall events (greater than 1" in a 24 hour period) shall be sampled at all stations to establish instream background conditions at higher flows. Storm events should be sampled at least two months apart during the year. The samples shall be taken at first flush usually within the first 30 minutes from the beginning of the storm event or when overland flow of rainfall has created flow in the stream. If no instream flow has been created by the storm event, a sample should not be taken. Stream substrate at known habitats shall be sampled for stream substrate condition to approximate proportion of substrate particle size and type according to Division's SOP manual once per year. Fine sediment deposition and transport shall be evaluated once per year in July by methods set forth in "Methods to Measure Sedimentation of Spawning Gravels" (Lisle and Eads 1991) published in a U.S. Forest Service Research Note PSW-411 at these same transects. The second year sampling shall include: 1) Aquatic macroinvertebrate community trend assessment sampling shall take place twice (July or August and October) in the second year of initial pre-mining data collection. The collection of macroinvertebrates and the analysis of the data shall follow Division of Water SOP manual. A non-invasive method of macroinvertebrate collection (such as Surber sampler or the meter2 kicknet method) will be used at all times so as not to disturb juveniles or collect young of year in riffle and run areas of the stream. A suitable rapid bioassessment protocol shall be performed on tributary streams to evaluate the biotic health and importance of these streams. If the Division has reason to believe that these tributary streams are ecologically important to the mainstem, then additional biological stations will be required to be added to the study plan to monitor these segments. Stream substrate at known habitats shall be sampled for stream substrate condition to approximate proportion of substrate particle size and type according to Division's SOP manual once per year. Fine sediment deposition and transport shall be evaluated once per year in July by methods set forth in "Methods to Measure Sedimentation of Spawning Gravels" (Lisle and Eads 1991) published in a U.S. Forest Service Research Note PSW-411 at these same transects. 2) The total fish community shall be surveyed in the second year in September, October or November. Surveys for the Cumberland blackside dace shall continue as in the first year of sampling in these months. In addition, Karr's Index of Biotic Integrity (as refined by the Division of Water for Kentucky) shall be used in the assessment of the total fish community. 3) Water quality monitoring shall be collected on a monthly basis and continue at the same sites selected in the first year. Storm events shall be sampled also as in the first year. 4) All of the above information will be assessed by the consultant for Quality Assurance and Quality Control. This information shall be documented in the quarterly reports sent to the Division of Water. A assessments and results discussion page will be required in the report. Data Analyses 1) All biological and physical-chemical assessments will be reported quarterly. 2) All quarterly reports will be compiled into a final calendar year report. 3) All final reports will contain a yearly and seasonal comparison of all past reported data. This comparison will include but not limited to water quality data, macroinvertebrate metric assessments and fish IBI scores and assessments. 4) All stations will be identified by latitude and longitude in decimal degrees. Mile points will also be given for each station. 5) The metrics that will be used to determine stream health will include but will not be limited the following metrics for mcroinvertebrates: Taxa richness; Total Number of Individuals; EPT; %EPT; HBI; %Dominance5; %Ephemeroptera; %Primary Clingers; and %Chironomidae. For Fish: Index of Biotic Integrity(IBI) based on Kentucky Division of Water assessments and reevaluations. 6) A CD or 3.5 Floppy disk in an Excel Spreadsheet format shall be included with the aforementioned yearly report with all past pre- mining sampling data included with the current sampling year’s data. Subsequent data floppy or CD’s will include only the current sampling year. Data that shall be included but not limited to: Date of sampling; Time of sampling; Station ID, Number and all pertinent site information; Sampler ID; Method of sampling; Macroinvertebrates, fish, and water quality data in separate files and associated metrics with each site; and habitat information sheets. All biological assessments and water quality shall continue to be conducted during the life of the permit until Phase 2 bond release.