HOW TO KEEP A LABORATORY BOOK by rfh21070

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									                     HOW TO KEEP A LABORATORY BOOK




INTRODUCTION

   Lab books are a property of the lab. A lab book is used to record what is done in
    the lab as the experiments are performed. The exact format may vary, but all lab
    notebooks should be clearly written and comprehensive in order to allow any
    other person to repeat the experiment without any other source of information.

   In principle your lab book is a legal document that can be used as evidence of
    your work in a court of law.

   It is a scientific document therefore you should avoid writing in 1 st, 2 nd person.
    Instead of “I loaded 10μl..” write “10 μl were loaded…or…add 10 μl to the mix”

   Always keep ONE lab book and write in ink so that if there are questions about
    the validity of your findings, you can prove that the work is original and was not
    falsified.

   Even if you make mistakes you should not use eraser. If you need to correct a
    mistake, strike a single line through the error example: RNA from human mouse.
    Different colored pens can be used to differentiate data, notes, drawings, graphs,
    and calculations from one another

   You should NEVER tear a page!!!

   You are expected to write your lab book in ENGLISH!
   You should write in your lab book EVERY DAY and not once a week about the
    last week’s experiments. WRITE WHILE YOU ARE DOING THE
    EXPERIMENT.



      20/10/07           21/10/07            17/10/07

                                                                              WRONG




            Page 1           Page 2            Page 3
    .


   START by writing
        a. the date
        b. what you are going to do (brief heading)
        c. why you are doing it (purpose of experiment)
        d. how you are doing it (method)


   METHODS- PROTOCOLS: You should write the method (appropriate
    protocol) at least once in order to repeat it when necessary. Next time you repeat
    the method you can write “repeat RNA extraction according to protocol” you do
    not have to re-write the whole protocol every time BUT you should clearly
    indicate any adjustments you make to it i.e: volume changes, different enzyme
    used etc.
EXAMPLE
Τuesday 30 November 2007

                     Reverse transcription for samples #C1 and #C2



Purpose: make cDNA for controls #1 and #2                            ALWAYS write the
                                                                     concentrations of the
                                                                     reagents you are using
Mix : RNA (used 2γ)                                 2 ul

       Rand. Hexamers (50ng/ul)                     2 ul

       dNTPs mix (10mM)                             4 ul

       DEPC-water                            8 ul

                               Total volume 16 ul



And add ….RTase mix after RNA denaturing

RTase mix: 10x RT buffer (stratagene)                      2 ul

                 RNase inhibitor (40u/ul)           1 uλ

                 RTase                       1 ul

                               TOT VOL.             4ul



Programme

1) 72oC for 4 min: denature of RNA

Stop reaction and put tubes on ice and add RTase mix

2) 42oC for 60 min

3) 90oc for 10 min

4) 4oC forever

                                    PCR reaction



Tube 1: template #C1 +mix

Tube 2: negative control       etc……..
                                                             ALWAYS write the
                                                             order that you have
                                 Run 2% agarose gel          loaded your samples



Lane 1: #C1

Lane 2: negative control
   AT THE END OF EACH EXPERIMENT ALWAYS WRITE RESULTS
    AND OBSERVATIONS AS WELL AS FUTURE EXPERIMENTS

   Attach in your lab book, if possible, gel photos, ELISA reading, otherwise write
    down name of file that you have saved your data. ALWAYS BACKUP YOUR
    FILES. You do not want to lose your work if the computer crashes!!!!!!


example
Result: No detectable band for VEGF for sample #1

Possible cause: amount of cDNA loaded?

Note: Load more cDNA next time




       FAQ: “ How much detail should I put in my lab book?”
       Answer: You should be able to understand WHAT you did 6 months ago,
        HOW and WHY and more importantly another person should be able to
        understand your work just by reading your lab book.


Just think that when writing your PhD thesis you will have to write about
experiments performed 3 years ago!!!!!!!!!!!!!!!!!




                          «Elephant’s memory does not help!»

								
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