Biology 312 – General Microbiology
Lab Report for Winogradsky Column
Your lab report should be 2-3 pages arranged into the following sections:
1. Title Page
3. Observations and Discussion
Section 1: Title Page
The Course name and number
Title of Project
Names of the members in your group, and the bench number of your Group
Starting and ending dates for the whole project Starting and ending dates for the whole project
Sources of mud and water used in the column.
Section 2: Introduction
Describe a Winogradsky column in general terms. Explain how you can use the Winogradsky column to
demonstrate dynamic interactions of microorganisms all growing in the same, limited space with limited nutrient
sources, and alternative environmental conditions, etc. Explain what it is, and why is it useful to study the diversity
of microorganisms in nature? How does it work in terms of growth dynamics? Why do you think different species
might rise and fall in the column? How might populations of species, all struggling to survive and reproduce,
interact in the column, and how might these interactions change the properties of the column? Indicate what
organisms (genus and species if applicable) are commonly found in columns; explain the depth and atmospheric
conditions that determine where the organisms grow. Describe what observations might be made (gas, color, etc)
and what biological process might be at work to produce these changes. Indicate what organisms might be found in
the various zones and colors.
Section 3: Observations and Discussion
1. Keep dated notes of your exercise as the quarter progresses. Record everything done to your column. Date
set-up, date foil removed, date light put on it, etc. Indicate the date and a description of your column
observations over time. You should include both microscopic and macroscopic observations. A drawing of
the column and microscopic observations should be included. See rubric.
2. After the presentation of your observations, you should provide a commentary of interpreting and
discussing the meaning and implications of the observations. This commentary constitutes the discussion,
and is an important part of the report. Based on the physical changes and microscopic observations, give
your best “guess” as to the particular microorganism you might suspect and/or observed in the various
zones and sampling areas.
Format: Most of the report should be typed, double spaced. Your observations taken during the quarter must be
handwritten and attached to the other sections. References should be listed at the end, but need not be cited in the
Grading: Grading will be based on originality, thoroughness of observations and presentation, and accuracy. The
report is worth 20 points.