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					Charity A. Holland, MPH
Last updated January 2005

EDUCATION: University of California at Berkeley Master of Public Health; Forensic Science – 1994 Villanova University Bachelor of Science in Biology – 1992 Minor in Sociology SKILLS: Serology (presumptive and confirmatory testing of blood and semen), DNA Extraction, Polymerase Chain Reaction, Gel Electrophoresis (ABI 377), Capillary Electrophoresis (ABI 3100), DNA Sequencing, Short Tandem Repeat Analysis (Profiler Plus/Cofiler, PowerPlex 16, PowerPlex Y), Mitochondrial DNA Analysis, Sequence Navigator Software, Genescan Software, Genotyper Software, Microsoft Office (Word, Excel, PowerPoint, Outlook) PROFESSIONAL EXPERIENCE: Mitotyping Technologies, LLC State College, PA Forensic Examiner

June 2005-present

The Bode Technology Group, Inc., a ChoicePoint Company Springfield, VA Forensic Technical Leader May 1999 – May 2005 *Supervise forensic DNA casework section (Serology, STR, Y-STR and mtDNA) *Perform forensic casework utilizing PCR, STR Technology, and Automated DNA Sequencing and Analysis of Mitochondrial DNA *Perform technical and administrative review for forensic DNA casework *Advise, consult, and assist investigative personnel and legal counsel regarding all aspects of physical evidence *Prepare and present material related to DNA at regional, national, and international conferences and seminars *Provide court testimony as an expert witness *Notable Projects: Identification of victims in the Alaska Air Flight 261 and American Airlines Flight 587 disasters, the World Trade Center Terrorist Attacks, for the International Commission on Missing Persons (Bosnia and Croatia), for the National Center for Missing and Exploited Children, and for Innocence Projects across the country Senior Forensic Analyst June 1998 – May 1999 Similar duties to the above, but without the formal responsibilities of analytical protocol management, proficiency testing program management, training program management, and quality assurance and quality control oversight for the assigned group.

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Charity A. Holland, MPH
Last updated January 2005

PROFESSIONAL EXPERIENCE (continued): Department of Defense DNA Registry Armed Forces DNA Identification Laboratory Rockville, MD DNA Analyst May 1994 – June 1998 *Performed identification casework utilizing PCR and Automated DNA Sequencing and Analysis of Mitochondrial DNA *Perform quality assurance and quality control procedures in support of casework *Reviewed Family Reference casework *Member of an assigned Mass Disaster Team *Prepared and presented servicemember identifications to family members *Notable projects: Identification of remains from the US Air Flight 427 disaster and the Tomb of the Unknown Soldier (Vietnam) Mercyhurst College Erie, PA Research/Laboratory Assistant June-August 1992 and 1993 *Assisted in human and faunal osteological analyses *Participated in a forensic anthropological investigation (June 1993) *Organized and coordinated Forensic Anthropology/Archeology Seminars Villanova University Villanova, PA Laboratory Assistant January 1990-May 1992 *Worked with two professors in the Biology Department assisting in genetics and molecular biology research CONFERENCES/CONTINUING EDUCATION: *American Academy of Forensic Sciences, 46th Annual Meeting, 14-19 February 1994, San Antonio, TX *International Symposium on Human Identification, Promega Corporation, 8-11 October 1994, Scottsdale, AZ *Accelerating Gene Discovery and Mutation Detection, Perkin Elmer, 17 May 1995 *American Academy of Forensic Sciences, 48th Annual Meeting, 19-24 February 1996 Nashville, TN *American Academy of Forensic Sciences Annual Meeting Workshop, Recovery and Identification of John and Jane Does, 19 February 1996 *American Academy of Forensic Sciences Annual Meeting Workshop, Advanced DNA Technologies, 20 February 1996 *Forensic Anthropology, Armed Forces Institute of Pathology, 13-17 May 1996 *National Expert Witness and Litigation Seminar, S.E.A.K. Publishing, Inc., 20-21 June 1996 *Second National Conference on the Future of DNA: Implications for the Criminal Justice System, 27-28 May 1997, Sacramento, CA *Statistical Genetics for Forensic Scientists, Dr. Bruce Weir, 8-10 September 1997

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Charity A. Holland, MPH
Last updated January 2005

*First International Workshop on Human Mitochondrial DNA, October 1997, Washington, D.C *American Academy of Forensic Sciences, 50th Annual Meeting, 9-14 February 1998, San Francisco, CA *International Symposium on Human Identification, Promega Corporation, October 1998, Orlando, FL *Advanced Fluorescent - STR MegaPlex Technology Workshop, Bode Technology Group, Inc., Promega Corporation, Hitachi Software Engineering America, Ltd., 21 -24 March 1999 *Mid-Atlantic Association of Forensic Scientists, 21-23 April 1999, Ocean City, MD *International Symposium on Human Identification, Promega Corporation, September 1999, Orlando, FL *Mitochondrial DNA Sequence Analysis in Forensic Casework: Methods and Issues Workshop, International Symposium on Human Identification, Promega Corporation, 29 September 1999 *American Academy of Forensic Sciences, 52nd Annual Meeting, 21-26 February 2000, Reno, NV *Discovery Issues for Forensic Laboratories Workshop, American Academy of Forensic Sciences Annual Meeting Workshop, 21 February 2000 *Advanced Fluorescent - STR MegaPlex Technology Workshop, Bode Technology Group, Inc., Promega Corporation, Hitachi Software Engineering America, Ltd., 12-17 March 2000 *Cambridge Healthtech Institute DNA Forensics, June 2000, Springfield, VA *American Academy of Forensic Sciences, 53rd Annual Meeting, 19-24 February 2001 Seattle, WA *MAAFS/SAFS Annual Meeting, 24-27 April 2001, Williamsburg, VA *European-American Intensive Course in Clinical and Forensic Genetics, 3-7 September 2001, Dubrovnik, Croatia *Forensic DNA Statistics Seminar, Dr. Kevin McElfresh, 31 January 2002 *American Academy of Forensic Sciences, 54th Annual Meeting, 11-16 February 2002, Atlanta, GA *Advanced DNA Technology Workshop, 20-23 May 2002, Duck Key, Florida *International Symposium on Human Identification, Promega Corporation, 7-10 October 2002, Phoenix, AZ *Advanced DNA Technology Workshop, 12-13 May 2003, Duck Key, Florida *The Third European-American School in Forensic Genetics, 1-5 September 2003, Zagreb, Croatia *Advanced DNA Technology Workshop, 8-11 March 2004, San Diego, CA *Sperm Search Training Lecture, Todd Bille, 7 April 2004 *Paternity Statistics (2 parts), Dr. Robert Bever, 21 April 2004, 6 May 2004 *Hair Biology, Dr. Charles Linch, 12 May 2004 * International Symposium on Human Identification, Promega Corporation, 4-7 October 2004, Phoenix, AZ *Y-STR Workshop: Practical Considerations and Interpretation Issues, 7 October 2004

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Charity A. Holland, MPH
Last updated January 2005

PROFESSIONAL ORGANIZATIONS: American Academy of Forensic Sciences – Member PRESENTATIONS AND ABSTRACTS: Dirkmaat, D.C., Diefenbach, C.A., Burkett, C., Mertz, C.A., and L. Sienicki. 1994 “Suicide or Homicide: A Multi-disciplinary Approach to the Analysis of a „Mummified‟ Hanging Victim.” Presented at 46 th Annual Meeting of the American Academy of Forensic Sciences, San Antonio, TX. “DNA Collection and Recovery Techniques” Charity A. Diefenbach, Presented at Forensic Anthropology Short Course (Part I), Mercyhurst College, Erie, PA May 1996 “DNA Collection and Recovery Techniques” Charity A. Diefenbach, Presented at Forensic Anthropology Short Course (Part II), Mercyhurst College, Erie, PA Aug. 1996 “New DNA Technologies – Use of mtDNA in Human Remains Identification” Charity A. Diefenbach, MPH Presented at El Instituto Nacional De Ciencias Penales, Mexico City, Mexico. 29 April 1997 “DNA Collection and Analysis” Charity A. Diefenbach, MPH Presented at Field and Laboratory Methods in the Recovery of Human Remains in an Outdoor Setting, Mercyhurst College, Erie, PA. 23 May 1997 “DNA in the Forensic Setting: Collection and Preservation Methodologies” Charity A. Diefenbach, MPH Presented at American Academy of Forensic Sciences Annual Meeting, San Francisco, CA 14 February 1998 “Methods in DNA Recovery and Interpretation” Charity A. Diefenbach, MPH Presented at Field and Laboratory Methods in the Recovery of Human Remains in an Outdoor Setting, Mercyhurst College, Erie, PA. 30 May 1998 Charity A. Diefenbach, MPH, Justin D. Karjala, MFS, Danielle R. Blecha, MFS, Robert A. Bever, PhD, and Kevin C. McElfresh, PhD. “Procedural Improvements in Mitochondrial DNA Analysis: Validation and Implementation of a New Hair Extraction Procedure and a Modified Amplification/Sequencing Primer” Poster Presentation at Ninth International Symposium on Human Identification, Promega Corporation. 8 October 1998 Y. Daoudi, M. Morgan, C. Diefenbach, J. Ryan, T. Johnson, G. Conklin, K. Duncan, K. Smigielski, E. Huffine, D. Rankin, R. Mann, T. Holland, K. McElfresh, J. Canik, V. Armbrustmacher, and M. Holland. “Identification of the Vietnam Tomb of the Unknown Soldier, The Many Roles of Mitochondrial DNA” Poster Presentation at Ninth International Symposium on Human Identification, Promega Corporation. 8 October 1998

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Charity A. Holland, MPH
Last updated January 2005

Kevin C. McElfresh, Debbie DiPierro, Robert Bever, Lisa Barnes, Charity Diefenbach, Liz Curry, Justin Karjala, Danielle Blecha, Dawn Harms, Lauren Brinkac, Christina O‟Kernick, and Laura Swan. “Putting STR Megaplexes to the Test: Casework, Felon Databanking, and Population Genetics” Presented at Ninth International Symposium on Human Identification, Promega Corporation. 8 October 1998 Yasser Daoudi, BS; Molly A. Morgan, MFS; Charity A. Diefenbach, MPH; John H. Ryan, PhD; Tracy M. Johnson; Gail M. Conklin, MFS; Kimberly Duncan; Kimberly B. Smigielski, MFS; Edwin F. Huffine, MS; Denise K. Rankin, BS; Robert W. Mann, MA; Thomas D. Holland, PhD; Kevin C. McElfresh, PhD; James J. Canik; Vernon Armbrustmacher, MD; and Mitchell M. Holland, PhD. “The End of an Era? A Tribute to the Vietnam Tomb of the Unknown” Presented at American Academy of Forensic Sciences Annual Meeting, Orlando, FL 19 February 1999 “Validation and Implementation of Mitochondrial DNA Sequence Analysis for Forensic Casework” Robert Bever, PhD, Justin Karjala, MFS, Danielle Blecha, MFS, Charity Diefenbach, MPH, Kevin McElfresh, PhD. Presented at American Academy of Forensic Sciences Annual Meeting, Orlando, FL 19 February 1999 “Identification and Forensic Casework Applications” Charity A. Diefenbach, MPH, Justin D. Karjala, MFS, Danielle R. Blecha, MFS, Robert A. Bever, PhD, and Kevin C. McElfresh, PhD. Presented at Mid-Atlantic Association of Forensic Scientists, Ocean City, MD 22 April 1999 “Mitochondrial DNA Casework Applications” Charity A. Diefenbach, MPH. Presented at International Symposium on Human Identification, Promega Corporation Mitochondrial DNA Sequence Analysis in Forensic Casework: Methods and Issues Workshop. 29 September 1999 “Extraction of Forensic DNA Samples” Charity A. Diefenbach, MPH. Presented at Third Annual Fluorescent STR Megaplex Technology Workshop, Hilton Head, SC. 16 March 2000 “DNA Profiling with Short Tandem Repeat (STR) Technology: Success with Outdoor Crime Scene Evidence” Charity A. Diefenbach, MPH, Lisa M. Barnes, BS, Suzanna R. Ulery, MFS and Kevin C. McElfresh, Ph.D. Presented at Cambridge Healthtech Institute‟s 4th Annual DNA Forensics Meeting, Springfield, VA. 2 June 2000 “High Throughput STR Analysis of Skeletal Remains in Mass Fatality Incidents” Charity A. Holland, MPH. Presented at European-American Intensive Course in Clinical and Forensic Genetics, Dubrovnik, Croatia. 3 September 2001 “Forensic DNA Casework – Innocence Project” Charity A. Holland, MPH. Presented at the Advanced DNA Technology Workshop, Duck Key, FL. 22 May 2002

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Charity A. Holland, MPH
Last updated January 2005

“A Quality, High-Throughput DNA Extraction Method for the Identification of Human Skeletal Material: Assisting in the Identification of Victims from the WTC and American Airlines Flight 587 Disasters” Mitchell Holland, Ph.D., Todd Bille, MS, Charity Holland, MPH, Chris Cave, MS, Robert Shaler, Ph.D., Howard Baum, Ph.D., Metchild Prinz, Ph.D., and Marie Samples, MS. Presented at the International Symposium on Human Identification, Promega Corporation. 8 October 2002 “Forensic DNA Casework – Innocence Project” Charity A. Holland, MPH. Presented at the International Symposium on Human Identification, Promega Corporation. 9 October 2002 “The World Trade Center DNA Identification Project: Utilization of an Advanced Extraction Procedure and Novel Mini-STR Multiplexes” Charity A. Holland, MPH. Presented at the Third European-American School in Forensic Genetics, Zagreb, Croatia. 3 September 2003 Charity A. Holland, Sylvia Gill, Faith Love Patterson, Mitchell M. Holland, and James W. Schumm. “Uncovering the Hidden Suspect: Practical Casework Experiences using the Promega PowerPlex Y System” Poster Presentation at the International Symposium on Human Identification, Promega Corporation. 6 October 2004

PUBLICATION: Holland MM, Cave CA, Holland CA, Bille TW. Development of a Quality, High Throughput DNA Analysis Procedure for Skeletal Samples to Assist with the Identification of Victims from the World Trade Center Attacks. Croat Med J 2003;44(3):264-272.

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